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Cell collection shaking table and cell collection method

A cell and collection area technology, applied in the field of biological products, can solve the problems that the intracellular virus collection device cannot meet the collection requirements, low production efficiency, and long time consumption, and achieve the effect of solving the problem of individual differences and uniform and stable harvest

Inactive Publication Date: 2019-04-16
SINOVAC DALIAN VACCINE TECH
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Problems solved by technology

[0006] The present invention aims to solve at least one of the technical problems existing in the prior art or related technologies: the existing intracellular virus collection device and collection method cannot meet the collection requirements during large-scale cultivation of cell factories, and there are low artificial collection rates, Problems of long time consumption and low production efficiency

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  • Cell collection shaking table and cell collection method
  • Cell collection shaking table and cell collection method
  • Cell collection shaking table and cell collection method

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Embodiment Construction

[0029] The specific embodiments of the present invention will be described in further detail below with reference to the accompanying drawings and embodiments. The following examples are intended to illustrate the present invention, but not to limit the scope of the present invention.

[0030] combine Figure 1-Figure 5 As shown, the present invention provides a preferred embodiment of a cell collection shaker, including a box body 1, a drive device 4, and a tray assembly 3. The box body 1 is provided with a closed collection area 12, and the tray assembly 3 is arranged in the collection area 12. Inside, the cell factory 2 is set on the tray assembly 3, and the drive device 4 drives the tray assembly 3 to rotate circumferentially with a set radius.

[0031] The collection area 12 in the box 1 provides a sealed space for the cell factory 2 , reduces the interference of the cell factory 2 by the environment, and ensures the stability of the cells in the cell factory 2 . The tr...

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Abstract

The invention relates to the field of biological products, in particular to a cell collection shaking table and a cell collection method. The invention provides the cell collection shaking table, which comprises a box body, a driving device and a tray assembly, wherein a closed collection area is arranged in the box body, the tray assembly is arranged in the collection area, a cell factory is arranged on the tray assembly, the driving device drives the tray assembly to rotate circumferentially with a set radius; the invention also provides a cell collection method adopting the cell collectionshaking table, wherein after the cells are cultured in the cell factory, the cell factory is fixed on the tray assembly, the tray assembly rotates for a set time with a set radius and a set rotating speed, and the set rotating speed is 100-300r / min. The cell collection shaking table and the cell collection method are suitable for cell collection in a cell factory and have simple structure, simpleand convenient operation, high collection efficiency and good collection effect.

Description

technical field [0001] The invention relates to the field of biological products, in particular to a cell collection shaker and a cell collection method. Background technique [0002] In the process of vaccine preparation, it is necessary to collect various virus particles, especially for the collection of intracellular viruses. The collection process is complicated and the collection effect is different. Among them, varicella virus, rotavirus, EV71 virus, CA16 virus, hepatitis A virus, etc. belong to Intracellular viruses have strong cell-binding properties, and cells must be collected and disrupted to obtain free virus particles when the virus is harvested. Among the current marketed products for the above viruses, most manufacturers use the freeze-thaw method to collect and disrupt cells. This method is suitable for culture methods such as spinner flasks and Klinefelter flasks, but is not suitable for cell factory culture systems; some manufacturers use EDTA or The tryps...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/26
CPCC12M33/08
Inventor 于巍秦旌程沙雪艳王贵强丁冬干玲玲宋俐霏孟凡红黄金凤
Owner SINOVAC DALIAN VACCINE TECH
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