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A dual-PCR detection method for sugarcane white leaf disease phytoplasma and Xanthomonas albus and its primer set

A technology of Xanthomonas and phytoplasma, applied in the field of plant protection, can solve the problems of labor-intensive and time-consuming detection process, low specificity, etc., and achieve the effect of improving accuracy, strong specificity, and improving detection efficiency

Active Publication Date: 2019-10-18
SUGARCANE RES INST OF YUNNAN ACADEMY OF AGRI SCI
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Problems solved by technology

[0005] In order to solve the sugarcane white leaf disease caused by phytoplasma and the sugarcane white streak disease caused by Xanthomonas albicans are very similar in symptoms and manifestations, two PCR reactions must be carried out to detect the two diseases separately when confirming the two diseases. pathogen, specificity is not high, and the technical problem of time-consuming and labor-intensive detection process, the present invention provides a fast, accurate and specific double PCR detection method of sugarcane white leaf disease phytoplasma and Xanthomonas albostratum and its specificity Primer sets, kits

Method used

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  • A dual-PCR detection method for sugarcane white leaf disease phytoplasma and Xanthomonas albus and its primer set

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Embodiment

[0022] 1. Primer Design

[0023] According to the sugarcane white leaf disease phytoplasma tuf gene sequence design is used to detect the tuf gene specific primer of sugarcane white leaf disease phytoplasma, the expected amplification fragment size is 290bp, described tuf gene specific primer is made of tuf-SF primer and tuf- SR primer composition, the target fragment length is 290bp, the nucleotide sequence of the tuf-SF primer is shown in SEQ ID NO: 1, and the nucleotide sequence of the tuf-SR primer is shown in SEQ ID NO: 2.

[0024] According to the rpoD gene sequence of Xanthomonas albostripes, the rpoD gene-specific primers for detecting Xanthomonas albostripes are designed, and the expected amplified fragment size is 498bp, and the rpoD gene-specific primers consist of rpoD-SF primers and rpoD-SR The primer composition, the target fragment length is 498bp, the nucleotide sequence of the rpoD-SF primer is shown in SEQ ID NO: 3, and the nucleotide sequence of the rpoD-SR ...

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Abstract

Disclosed are a double PCR detection method for sugarcane white leaf disease phytoplasma and Xanthomonas albilineans and a primer set thereof. The present invention designs a pair of specific primers for the tuf gene of sugarcane white leaf disease phytoplasma and the rpoD gene of Xanthomonas albilineans, respectively, and by means of optimization, establishes a double PCR reaction system that is capable of simultaneously detecting the sugarcane white leaf disease phytoplasma and the Xanthomonas albilineans.

Description

technical field [0001] The invention belongs to the technical field of plant protection, and in particular relates to the detection of sugarcane white leaf disease phytoplasma and xanthomonas albostratum by double PCR technology and the specific primer group used. Background technique [0002] Sugarcane white leaf disease caused by Sugarcane white leaf phytoplasma and sugarcane white streak disease caused by Xanthomonas albilineans (Ashby) Dowson are both devastating diseases of sugarcane, causing and huge economic losses to the sugar industry. The main symptoms of sugarcane white leaf disease are leaf albinism, increased tillers and dwarfing. The main symptoms of sugarcane white streak disease are white stripes on the leaves, some of the tip leaves are albino, and the lateral buds sprout, and the germinated lateral bud leaves are also white. The symptoms of these two diseases are very similar, that is, leaf albinism. Therefore, it is difficult to accurately distinguish th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/689C12Q1/686C12N15/11
CPCC12Q1/686C12Q1/689C12Q2537/143
Inventor 张荣跃黄应昆李文凤王晓燕李婕单红丽仓晓燕尹炯罗志明
Owner SUGARCANE RES INST OF YUNNAN ACADEMY OF AGRI SCI
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