Low-temperature quick micro-freezing preservation technology for human sperms and eggs

Abstract

The invention provides a low-temperature quick micro-freezing preservation technology for human sperms and eggs. The low-temperature quick micro-freezing preservation technology for the human sperms and eggs adopts a high-tech biological technology, the ice crystal condition of organism cells under a low-temperature condition is studied, a low temperature and ultra-low temperature technology and anewly-invented micro-freezing fluid from natural components are applied to the human sperms and eggs frozen for preservation, an innovative freezing curve is applied, and the human sperms and eggs are directly frozen in the micro-freezing fluid for preservation. Freezing preservation of the human sperms and eggs is completed in 15-30 minutes, which is presented in the respects that cell membranesof the human sperms and eggs after the freezing preservation are not frosted to be cracked and are under an organism micro-freezing condition; and then refrigerating is performed so as to maintain the human sperms and eggs after the freezing preservation unchanged in quality. The low-temperature quick micro-freezing technology is an innovative micro-freezing preservation technology in the world,the cells of the human sperms and eggs are kept not cracked, and activity and antibodies of the human sperms and eggs are improved; and when freezing preservation is performed on the human sperms andeggs in the shortest time according to the freezing curve, after the human sperms and eggs are stored under the refrigerating condition for within 10 years, the activity of the human sperms and eggs can be kept unchanged.

Description

1. Technical field

[0001] The invention relates to the technical field of cryopreservation of human sperm and eggs, and proposes a low-temperature rapid micro-freezing preservation technology of human sperm and eggs. 2. Background technology

[0002] We have found that in nature, the somatic cells of hibernating animals have a natural antifreeze mechanism, such as frogs, loach, crucian carp, and other animals and aquatic animals that live in subtropical and frigid regions. The polysaccharide antifreeze substances of somatic cells have a sharp increase in the number of double bonds of fatty acid molecules in their subcellular membranes. The antifreeze is studied from the perspective of cell dynamics, so that the somatic cells are not frozen due to the drop in external temperature. Crystal frost heaves and ruptures; organisms preserve their living bodies by keeping their body cells in a slightly frozen state, and recover and revive when the temperature rises. The evolution of...

Examples

Embodiment Construction

[0009] Hereinafter, the invention will now be described more fully with reference to the accompanying drawings, in which various embodiments are shown. However, this invention may be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art.

[0010] Hereinafter, exemplary embodiments of the present invention will be described in more detail with reference to the accompanying drawings.

[0011] combined with figure 1 As shown, the human sperm and ovum low-temperature rapid micro-freezing preservation technology of the present invention is composed of human sperm and ovum screening 1, sterilization 2, vacuum packaging 3, continuous micro-freezing machine (-27 ℃--30℃) 500-1000g / bag micro-frozen for 20-30 minutes 4. Check the temperature of human sperm and egg...

1. Technical field

[0001] The invention relates to the technical field of cryopreservation of human sperm and eggs, and proposes a low-temperature rapid micro-freezing preservation technology of human sperm and eggs. 2. Background technology

[0002] We have found that in nature, the somatic cells of hibernating animals have a natural antifreeze mechanism, such as frogs, loach, crucian carp, and other animals and aquatic animals that live in subtropical and frigid regions. The polysaccharide antifreeze substances of somatic cells have a sharp increase in the number of double bonds of fatty acid molecules in their subcellular membranes. The antifreeze is studied from the perspective of cell dynamics, so that the somatic cells are not frozen due to the drop in external temperature. Crystal frost heaves and ruptures; organisms preserve their living bodies by keeping their body cells in a slightly frozen state, and recover and revive when the temperature rises. The evolution of...

Examples

Embodiment Construction

[0009] Hereinafter, the invention will now be described more fully with reference to the accompanying drawings, in which various embodiments are shown. However, this invention may be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art.

[0010] Hereinafter, exemplary embodiments of the present invention will be described in more detail with reference to the accompanying drawings.

[0011] combined with figure 1 As shown, the human sperm and ovum low-temperature rapid micro-freezing preservation technology of the present invention is composed of human sperm and ovum screening 1, sterilization 2, vacuum packaging 3, continuous micro-freezing machine (-27 ℃--30℃) 500-1000g / bag micro-frozen for 20-30 minutes 4. Check the temperature of human sperm and egg...

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