Method for the microbial production of specific natural capsaicinoids

一种辣椒素、辣椒的技术，应用在生物化学设备和方法、产生脂肪、使用载体引入外来遗传物质等方向，能够解决有毒废物处理等问题

Active Publication Date: 2019-05-21
CONAGEN INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, solvent extraction itself is energy intensive, causes problems with toxic waste disposal, requires large areas for the plants themselves to grow and produces products that require further purification to recover small amounts of components

Method used

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  • Method for the microbial production of specific natural capsaicinoids
  • Method for the microbial production of specific natural capsaicinoids
  • Method for the microbial production of specific natural capsaicinoids

Examples

Experimental program
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Effect test

example 1

[0110] 500 mg / L vanillin amine and 500 mg / L individual fatty acids were fed to E. coli cultures overexpressing the ACS1 and CS genes of ghost pepper. Produced samples were taken one (1) and two (2) days after substrate feed and analyzed by high performance liquid chromatography (HPLC) (see figure 2 ).

[0111] Such as figure 1 and figure 2 As shown, the result of this experiment was the production of nonanoylamide and other capsaicinoids. NV is the amide of geranic acid (n-nonanoic acid) and vanillyl nonamide. Vanillyl nonamide is widely used as a spicy food additive and as a medicine for arthritis and muscle pain relief.

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Abstract

The present invention relates to the production of capsaicinoid compounds including Capsaicin and Nonivamide via microbial fermentation.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to US Provisional Application No. 62 / 363,951, filed July 19, 2016, the contents of which are incorporated herein by reference in their entirety. [0003] Incorporation of Sequence Listings [0004] The Sequence Listing contained in the file entitled "C1497.70006WO00.txt" created on June 27, 2016 was simultaneously filed electronically (using the U.S. Patent Office's EFS-Web file submission system) and is incorporated by reference in its entirety In this article. technical field [0005] The field of the invention relates to methods and processes useful for the microbial production of capsaicin. More specifically, in some embodiments, the present invention provides a method for producing vanillyl nonamide and other specific capsaicinoids at yields not otherwise possible in plant-based systems. The present invention provides, inter alia, a genetically modified E. coli strain modified ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/21C12N15/70C12N9/00
CPCC12N9/1051C12N9/93C12P13/02A61P25/02A61P29/00A61P29/02A23L27/70A61K31/165A23L27/10A23V2002/00A61K9/0014C07C233/18C07C233/20C11B9/0061C12Y203/01086
Inventor H.陈X.于L.周H.王M.王
Owner CONAGEN INC
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