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Application of favipiravir in the preparation of drugs for inhibiting the proliferation of canine distemper virus

A canine distemper virus and favipiravir technology, applied in the field of medicine, can solve the problem that no favipiravir has inhibited the measles virus, and achieve the effects of good antiviral efficacy, low toxicity and significant antiviral efficacy

Active Publication Date: 2020-03-13
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far there has been no report on the inhibition of measles virus by favipiravir

Method used

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  • Application of favipiravir in the preparation of drugs for inhibiting the proliferation of canine distemper virus
  • Application of favipiravir in the preparation of drugs for inhibiting the proliferation of canine distemper virus
  • Application of favipiravir in the preparation of drugs for inhibiting the proliferation of canine distemper virus

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: Favipiravir (T-705) canine distemper virus inhibition experiment

[0028] The experimental method is as follows:

[0029] 1. Drug configuration and dilution

[0030] T-705 was dissolved in DMEM medium at a final concentration of 5 mg / ml, and serially diluted to 1250, 625, 312.5, 156.25, 78.125 μg / ml, stored at 4 degrees, and used up within a week.

[0031] 2. Virus Titer Determination

[0032] Vero cells are introduced into the 96-well plate and grown to 80%-90%, and the virus sample is diluted into different dilutions by 10 times with cell maintenance solution (2% FBS in DMEM), starting from 10 -1 to 10 -8 , after aspirating the cell growth solution in the 96-well cell culture plate, inoculate Vero cells with different dilutions of virus, inoculate 100 μl per well, and place at 37°C, 5% CO 2 Cultured in the incubator for 3 days. The cell supernatant was discarded, and 100 μl of cold acetone was added to each well to fix at minus 20 overnight. Discar...

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Abstract

The invention relates to an application of favipiravir in preparation of a drug for inhibiting canine distemper virus proliferation. The in-vitro results of the present invention show that the addition of favipiravir (T-705) to Vero and DH82 cells infected with CDV-3 and CDV-11 can produce significant viral inhibition effect and demonstrate good antiviral efficacy, and as the concentration of thedrug increases, the antiviral effect gradually increases. After the cells are infected with the virus for 12 to 48 hours, the drug is added to exert significant antiviral effect, and the application of favipiravir (T-705) after exposure is very effective in inhibiting CDV from different animal sources.

Description

technical field [0001] The invention relates to the application of a virus inhibitor favipiravir, in particular to the application of favipiravir in the preparation of drugs for inhibiting the proliferation of canine distemper virus, and belongs to the technical field of medicine. Background technique [0002] Canine distemper (CD) is an acute, highly contagious disease caused by canine distemper virus (CDV) infecting carnivorous animals. The disease is highly contagious and has high morbidity and mortality. 1. Natural infection has a wide range of hosts, and its fatality rate has a great relationship with the host species. It often causes a large number of animals such as dogs, minks, and foxes to become ill. The fatality rate reaches 30-80%, and the mortality rate of ferrets is as high as 100%. The clinical symptoms caused by the disease are complex and diverse, which can be manifested as respiratory tract inflammation, digestive tract inflammation or nervous system sympto...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/4965A61P31/14
Inventor 郑学星朱业蕾薛付忠韩琳
Owner SHANDONG UNIV