T cell receptor targeting MAGE-A3, T cell receptor gene-modified T cell, preparation method and application thereof

A MAGE-A3, cell receptor technology, applied in the field of medical biology, can solve the problems of preparation and research of T cell receptors that do not target MAGE-A3, and achieve high affinity, high transcription efficiency, high infection The effect of efficiency

Inactive Publication Date: 2019-06-04
SHENZHEN BINDEBIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is still a challenge for TCR-T technology to obtain TCR genes with high specificity and high affinity for tumor antigens and the correct pairing of TCR subunits (such as α chain and β chain)
At present, there is no preparation and research related to the T cell receptor targeting MAGE-A3

Method used

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  • T cell receptor targeting MAGE-A3, T cell receptor gene-modified T cell, preparation method and application thereof
  • T cell receptor targeting MAGE-A3, T cell receptor gene-modified T cell, preparation method and application thereof
  • T cell receptor targeting MAGE-A3, T cell receptor gene-modified T cell, preparation method and application thereof

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Experimental program
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Embodiment 1

[0057] A method for preparing T cell receptor gene-modified T cells targeting MAGE-A3, comprising the following steps:

[0058] (1) Prepare the gene sequence of the T cell receptor TCR-MAGE-A3 targeting MAGE-A3

[0059] Prepare the coding gene sequences of α chain, 2A peptide and β chain respectively, the coding gene sequence of the α chain includes the nucleotide sequence shown in SEQ ID NO: 3, and the coding gene sequence of the β chain includes SEQ ID NO : the nucleotide sequence shown in 4, the coding gene sequence of the 2A peptide includes the nucleotide sequence shown in SEQ ID NO: 5.

[0060] The above-mentioned α-chain, 2A peptide and β-chain coding gene sequences were linked together sequentially from the 5' end to the 3' end by PCR method to obtain the coding gene of the T cell receptor TCR-MAGE-A3 targeting MAGE-A3 sequence.

[0061] (2) Construction of pLenti-TCR-MAGE-A3 recombinant plasmid

[0062] The coding gene sequence of TCR-MAGE-A3 was inserted between t...

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Abstract

The present invention provides a T cell receptor targeting MAGE-A3, the T cell receptor targeting MAGE-A3 comprises an alpha chain and a beta chain, the alpha chain comprises an amino acid sequence shown in SEQ ID NO: 1, and the beta chain comprises the amino acid sequence shown in SEQ ID NO: 2. The present invention also provides a T cell receptor gene-modified T cell targeting MAGE-A3. The T cell receptor gene-modified T cell targeting MAGE-A3 can efficiently and specifically kill MAGE-A3 positive cancer cells, has long-lasting vitality and lethality, and do not cause damage to normal cells.The invention also provides a preparation method of the T cell receptor gene-modified T cell targeting MAGE-A3 and an application thereof.

Description

technical field [0001] The invention relates to the field of medical biology, in particular to a T cell receptor targeting MAGE-A3, a T cell receptor cell, and a preparation method and application thereof. Background technique [0002] Malignant tumors are the most serious diseases that endanger human health. Every year, about 3 million people in China are diagnosed with new tumors, and 2 million people die of cancer. Human melanoma antigen-A3 (melanoma antigen-A3, MAGE-A3) is a member of the melanoma-associated antigen family. MAGE-A3 is always associated with tumors, and is mostly expressed in malignant tumor tissues mainly melanoma, and can be highly expressed in tumors of various tissue types, such as lung cancer, breast cancer, cervical cancer, gastric cancer, node- Rectal cancer, liver cancer, etc., but not in normal cells. [0003] In recent years, immune cell therapy has become a treatment method with great prospects in the treatment of malignant tumors after surge...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/725C12N15/12C12N5/10C12N15/867A61P35/00
Inventor 张长风吴咏东
Owner SHENZHEN BINDEBIOTECH CO LTD
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