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T cell receptor targeting TIPE3, T cell receptor gene-modified T cell, preparation method and application thereof

A cell receptor and gene modification technology, applied in the field of medical biology, to achieve high infection efficiency, transcription efficiency, and high affinity

Inactive Publication Date: 2019-06-04
SHENZHEN BINDEBIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is still a challenge for TCR-T technology to obtain specific and high-affinity TCR genes for tumor antigens and the correct pairing between TCR double chains
In addition, there is no preparation and research related to the T cell receptor targeting TIPE3

Method used

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  • T cell receptor targeting TIPE3, T cell receptor gene-modified T cell, preparation method and application thereof
  • T cell receptor targeting TIPE3, T cell receptor gene-modified T cell, preparation method and application thereof
  • T cell receptor targeting TIPE3, T cell receptor gene-modified T cell, preparation method and application thereof

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Experimental program
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Embodiment 1

[0058] A method for preparing T cell receptor gene-modified T cells targeting TIPE3, comprising the following steps:

[0059] (1) Prepare the gene sequence of the T cell receptor TCR-TIPE3 targeting TIPE3

[0060] Prepare the coding gene sequences of α chain, 2A peptide and β chain respectively, the coding gene sequence of the α chain includes the nucleotide sequence shown in SEQ ID NO: 3, and the coding gene sequence of the β chain includes SEQ ID NO : the nucleotide sequence shown in 4, the coding gene sequence of the 2A peptide includes the nucleotide sequence shown in SEQ ID NO: 5.

[0061] The coding gene sequences of the above-mentioned α chain, 2A peptide and β chain were connected sequentially from the 5' end to the 3' end by PCR method to obtain the coding gene sequence of the T cell receptor TCR-TIPE3 targeting TIPE3.

[0062] (2) Construction of pLenti-TCR-TIPE3 recombinant plasmid

[0063] The coding gene sequence of TCR-TIPE3 was inserted between the MluI and Ec...

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Abstract

The present invention provides a T cell receptor targeting TIPE3, the T cell receptor targeting TIPE3 comprises an alpha chain and a beta chain, the alpha chain comprises an amino acid sequence shownin SEQ ID NO: 1, and the beta chain comprises the amino acid sequence shown in SEQ ID NO: 2. The present invention also provides a T cell receptor gene-modified T cell targeting TIPE3. The T cell receptor gene-modified T cell targeting TIPE3 can efficiently and specifically kill TIPE3 positive cancer cells, has long-lasting vitality and lethality, and do not cause damage to normal cells. The invention also provides a preparation method of the T cell receptor gene-modified T cell targeting TIPE3 and an application thereof.

Description

technical field [0001] The invention relates to the field of medical biology, in particular to a T cell receptor targeting TIPE3, a T cell receptor cell and a preparation method and application thereof. Background technique [0002] Tumor necrosis factor α-inducible protein 8-like 3 (TNFAIP8L3, TIPE3) is a member of the TNFAIP3 family, which acts as a lipid second messenger transport protein to promote carcinogenesis, and acts as a feedback enhancer to regulate carcinogenesis and phospholipid pathways. TIPE3mRNA can be detected in many tissues, but TIPE3 protein is rarely expressed in normal tissues, but its expression is significantly increased in cancerous tissues. Therefore, TIPE3 is a very suitable tumor-specific target for cellular immunotherapy. [0003] In recent years, immune cell therapy has become a treatment method with great prospects in the treatment of malignant tumors after surgery, radiotherapy, and chemotherapy. Immune cell therapy has the great advantages...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/725C12N15/12C12N5/10C12N15/867A61P35/00
Inventor 张宏玲曾滢
Owner SHENZHEN BINDEBIOTECH CO LTD
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