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Functional fibrinogen activator detection, and application thereof

A technology of functional fiber and protein, applied in the direction of biological testing, material inspection products, etc., can solve the problems affecting the accuracy of results, increasing the influence of human factors, fibrinogen to be developed, etc.

Pending Publication Date: 2019-06-14
深圳优迪生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the existing methods for detecting fibrinogen are mostly based on optical methods. The detection principle is to measure the content of fibrinogen according to the change of turbidity in the coagulation process, which is easily affected by the turbidity in the blood sample itself. Material influence, thus affecting the accuracy of the results, at the same time, the samples need to be pretreated in advance, increasing the possibility of being affected by human factors during the detection process
[0008] Therefore, current assays for fibrinogen remain to be developed

Method used

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  • Functional fibrinogen activator detection, and application thereof
  • Functional fibrinogen activator detection, and application thereof
  • Functional fibrinogen activator detection, and application thereof

Examples

Experimental program
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Effect test

specific Embodiment

[0061] According to a specific embodiment of the present invention, the method includes the following steps:

[0062] S1, prepare a buffer solution of 10-100mmol / L, and adjust the pH value to 6-8;

[0063] S2, add 0.1-1 mass % salt and 1-20 mg / ml protective agent to the above S1 in sequence, and stir evenly;

[0064] S3, adding 1 to 10 mg / ml of platelet inhibitors efibate and tirofiban to the above S2;

[0065] S4, adding 0.1-1 mg / ml exogenous blood coagulation activator tissue factor to the above S3, stirring evenly;

[0066] S5, adding 0.01-0.1 mass % preservative to the above S4, stirring evenly, subpackaging and freeze-drying, and storing in an environment of 2-8°C.

[0067] Those skilled in the art can understand that the features and advantages described above for the functional fibrinogen activator are also applicable to the method for preparing the functional fibrinogen activator, and will not be repeated here.

[0068] Reagent test kit

[0069] In yet another aspe...

Embodiment 1

[0082] The present embodiment provides preparation of coagulation activators according to the following formula, including the following groups:

[0083] Experimental group 1 was prepared according to the following steps to obtain fibrinogen activator:

[0084] Step (1): Preparation of PBS buffer solution: Weigh 5 g of disodium hydrogen phosphate, 0.6 g of sodium dihydrogen phosphate, 8 g of sodium chloride, and 0.2 g of potassium chloride, add 1000 mL of purified water, stir to fully dissolve, and adjust the pH value to 7.0;

[0085] Step (2): add 20g bovine serum albumin in the buffer solution of step (1);

[0086] Step (3): Add 10 g of efibrate and 10 g of tirofiban to the solution in step (2), stir to make it fully mixed, and adjust the pH to 7.0-7.4;

[0087]Step (4): Add 1.0 g of tissue factor to the solution in step (3), stir to make it fully dissolve, and adjust the pH to 7.0-7.4;

[0088] Step (5): Add 1.0 g of Proclin300 and 0.5 g of gentamycin to the solution in ...

Embodiment 2

[0106] A kit is obtained in the following manner, which includes a fibrinogen activator, a calcium chloride solution, and a sample cup.

[0107] Wherein, the fibrinogen activators are the fibrinogen activators prepared by the experimental groups 1-3 respectively.

[0108] The concentration of the calcium chloride solution is 0.2M: Weigh 22.2g of calcium chloride and add it to 1000mL of normal saline, stir to dissolve it fully, and make a calcium chloride solution with a final concentration of 0.2M, and then divide it into 1mL / tube .

[0109] The sample cup is an empty container used for loading test samples and test reagents, including blood samples, fibrinogen activator and calcium chloride solution.

[0110] According to the above respectively, the first kit, the second kit and the third kit are obtained.

[0111] The kit was then refrigerated at 4°C.

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Abstract

The invention puts forward a functional fibrinogen activator, which comprises extrinsic coagulation activator, platelet inhibitor, buffer salt and protective agent. The functional fibrinogen activatordisclosed by the invention simultaneously contains the extrinsic coagulation activator and the platelet inhibitor, the aggregation of platelets can be inhibited while an extrinsic coagulation mechanism is activated, and in addition, salts and protective agent are simultaneously added into the activator to perform a function of guaranteeing coagulation. When the activator is adopted to carry out thromboelastography activated coagulation detection, the contents of fibrinogen in a blood sample can be accurately measured, and therefore, the functional fibrinogen activator has a wide application prospect.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnostic reagents, in particular to the detection and application of functional fibrinogen, in particular to a functional fibrinogen activator, a preparation method and a functional fibrinogen detection kit. Background technique [0002] Fibrinogen, coagulation factor I, is a dimer composed of αA, βB and v chains, with a molecular weight of 3,400,000 and 2,964 amino acids. The plasma content is 2.0-4.0g / L, and the biological half-life is 96-144h. It exists in the adsorbed plasma and can be converted into fibrin after being acted on by thrombin and factor ⅩⅡa. [0003] Fibrinogen is a glycoprotein synthesized by the liver. It is a dimer composed of αα, ββ, and γγ peptide chains composed of 610, 461, and 411 amino acids, respectively, with a molecular weight of 340,000. [0004] Fibrinogen can promote the aggregation of platelets, promote the growth, proliferation and contraction of smooth muscl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/86G01N33/68G01N33/53
Inventor 艾峰冯琼
Owner 深圳优迪生物技术有限公司
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