Application of novel gastric cancer marker circ-EIF4G3

A marker and molecular marker technology, applied in the field of tumor molecular biology, can solve problems such as poor screening compliance

Active Publication Date: 2019-07-09
江苏万成生物医学研究院有限公司
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  • Abstract
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Problems solved by technology

[0005] In order to solve the above problems, the present invention discloses the application of a new gastric cancer marker circ-EIF4G3, aiming at the current situation that the target population has poor compliance with endoscopic screening for gastric cancer, and most of the clinically diagnosed gastric cancer patients are in the middle and late stage It is proposed to find the ideal molecular early warning signal in the process of gastric cancer carcinogenesis, carry out targeted endoscopic examination, and make a pathological diagnosis, so as to alleviate the suffering of patients, avoid excessive medical treatment, and save medical resources

Method used

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  • Application of novel gastric cancer marker circ-EIF4G3

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Embodiment 1

[0031] 1. RNase R Digestion and Actinomycin-D Digestion

[0032] 2mg of total RNA was incubated at 37°C for 20 minutes with or without the addition of 3U / mg RNase R, followed by the use of the RNeasyMinElute kit. For actinomycin D treatment, 2 mg of total RNA was incubated at 37°C with or without 1 mg of actinomycin (actinomycin D, Sigma, Chengdu Best Reagent Company), and incubated at 0,6 , 12, and 18 hours to detect the resulting RNA.

[0033] 2. Patient and case screening

[0034]A total of 64 GC tissues and corresponding adjacent non-tumor tissue samples were collected from GC patients, and all tissue samples were obtained from the Department of General Surgery, Nanjing Hospital Affiliated to Nanjing Medical University (between January 2011 and December 2017). All enrolled patients received radiotherapy and chemotherapy before surgery, and their tissue samples were immediately stored in RNA fixation reagent and immediately placed in a -80°C freezer for preservation. Pai...

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Abstract

The invention discloses application of a novel gastric cancer marker circ-EIF4G3 and relates to detection and application of molecular markers circ-EIF4G3. By means of the gastric cancer marker circ-EIF4G3, a detection primer with specificity for real-time quantitative PCR is designed and synthesized. In order to change the current situation that the compliance of endoscope screening of target groups with gastric cancer is poor and clinically defined patients with gastric cancer almost stay in the middle and late period of gastric cancer, ideal molecular prewarning signals are found in a cancerization process of gastric cancer, and endoscopic examination and pathological diagnosis are carried out in a targeted mode so as to relieve the pain of the patients, avoid excessive medical treatment and save medical resources. According to the application of the gastric cancer marker, the molecular marker circ-EIF4G3 can be used for detecting and monitoring prognosis of the patients with gastric cancer.

Description

technical field [0001] The invention relates to the field of tumor molecular biology, in particular to the application of a new gastric cancer marker circ-EIF4G3. Background technique [0002] At present, gastric cancer still ranks third in cancer-related deaths worldwide and is one of the major health burdens of society. Gastric cancer is the second most prevalent cancer and the second leading cause of cancer death in China, with an estimated 679,100 new cases and 498,000 deaths in 2015. Despite advances in surgical techniques and combined chemotherapy regimens, the 5-year overall survival of gastric cancer is still below 30% in most countries because of the lack of early diagnostic markers for gastric cancer. Therefore, it is necessary to carefully explore gastric cancer-related molecular features and develop reasonable methods for early detection of gastric cancer. [0003] Circular RNAs (circRNAs), thought to arise from non-canonical splicing of linear pre-mRNAs, are w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/118C12Q2600/158C12Q2600/166C12Q2600/178
Inventor 康宁朱桂芳曹秀峰
Owner 江苏万成生物医学研究院有限公司
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