Construction method of MRFFT2 cells

A construction method and cell technology, applied in the biological field, can solve the problem of not considering T cell self-protection technology, etc., and achieve the effects of strong target specificity, improved lethality, and less off-target effect.

Active Publication Date: 2019-08-06
BEIJING DCTY BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] None of the above schemes considers the self-protection technology of T cells, so that a small number of specific T cells directly face the powerful tumor immune microenvironment

Method used

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  • Construction method of MRFFT2 cells
  • Construction method of MRFFT2 cells
  • Construction method of MRFFT2 cells

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Embodiment Construction

[0058]The present invention is described below through specific embodiments. Unless otherwise specified, the technical means used in the present invention are methods known to those skilled in the art. In addition, the embodiments should be considered as illustrative rather than limiting the scope of the invention, the spirit and scope of which is defined only by the claims. For those skilled in the art, on the premise of not departing from the spirit and scope of the present invention, various changes or modifications to the material components and dosage in these embodiments also belong to the protection scope of the present invention.

[0059] 1. Epitope prediction

[0060] 1) Use the peripheral blood of lung cancer patients for ctDNA sequencing and HLA typing detection;

[0061] 2) Use software to analyze the sequencing information: compare the ctDNA sequencing results with the genome of normal cells, and screen out the mutation sites;

[0062] 3) Take the mutated amino...

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Abstract

The invention relates to a construction method of MRFFT2 cells. an epitope is use for forecasting and screening out mutation polypeptide, and an expression gene sequence of the mutation polypeptide is connected and synthesized; besides, an MVA virus vector is constructed, an MVA virus is packaged, transfection on APCs (antigen presenting cells) is performed, the reformation of a specific MV cellis completed, the APCs and PBMCs separated from peripheral blood are co-cultured in vitro, and effective polypeptide is screened; through second impact with accurate effective polypeptide irritation,ordinary T cells are reformed into RFF cells having pinpoint killing capacity; and then through a TCR-T technique principle, reformation is performed, the reformed T cells are subjected to immunosuppression signal enclosing through an antibody medicine in vitro, specific killer T cells are accurately protected from in vivo restraint, and the lethality of the T cells on tumor cells is increased.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for constructing MRFFT2 cells. Background technique [0002] At present, in terms of tumor-specific immunotherapy, the existing LAK, DC, CIK, and DC-CIK cells and methods have basically been proved to be ineffective, while NK, CAR-NK, TIL and other cell technologies have yet to mature. CAR- T cells also have shortcomings in safety and solid tumor therapy. In the prior art, DCs are generally modified to present T cells to produce specific killing. Some laboratories are trying to use viruses as vectors to transfect and present T cells to induce specific killing of T cells. We have also used mutant hybrid peptides to directly stimulate PBMCs to induce T cells. There are also laboratories using TCR-T technology to target the presentation of MAGE A3 antigen. [0003] The above treatment methods are immature, especially the in vitro induction of DC cells and the te...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/90C12N5/10
CPCC07K14/7051C12N5/0638C12N15/907C12N2510/00C12N2800/80C12N2810/10
Inventor 焦顺昌张嵘宋玉洁周子珊解佳森袁翰李营营
Owner BEIJING DCTY BIOTECH CO LTD
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