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LFF1 cell

A cell and antigen epitope technology, applied in the biological field, can solve problems such as T cell self-protection technology, and achieve the effect of unlimited killing ability, less off-target effect, and high killing efficiency

Inactive Publication Date: 2019-04-26
BEIJING DCTY BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] None of the above schemes consider the self-protection technology of T cells, so that a small number of specific T cells directly face the powerful tumor microenvironment

Method used

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  • LFF1 cell
  • LFF1 cell
  • LFF1 cell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] This example will take lung cancer patients as an example to provide targeted LFF1 cells and their preparation methods:

[0037] 1. Whole Exome Sequencing

[0038] 1) Take peripheral blood from lung cancer patients for ctDNA sequencing and HLA typing;

[0039] 2) Use software to analyze the sequencing information: compare the ctDNA sequencing results with the genome of normal cells, and screen out the mutation sites;

[0040] 2. Epitope prediction

[0041] 1) Take the mutated amino acid site as the center, extend 8 amino acids to both sides, and use this 17-amino acid polypeptide as a potential epitope”;

[0042] 2) Use prediction software to analyze the IC50 of the potential antigenic epitope (recommended software: NetMHCpan 3.0, PickPocket, artificial neural networks (ANN)), if IC50<1000nM, the potential antigenic epitope is considered as "antigenic epitope";

[0043] 3. Peptide link

[0044] (1) Use software to analyze the IC50 at the junction of any antigen epit...

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Abstract

The invention belongs to the technical field of biotechnology, and particularly relates to an LFF1 cell and a preparation method thereof. According to the cell, after an antigen-presenting cell is transfected by using lentivirus for expressing antigen epitope peptide, a PBMC (peripheral blood mononuclear cell) is stimulated, then knockout of a suppressive signal molecular target is adopted for protection, and therefore the anti-tumor capacity of a T cell can be effectively improved.

Description

Technical field: [0001] The invention belongs to the field of biotechnology, and in particular relates to an LFF1 cell and a preparation method thereof. Background technique: [0002] Lentivirus (LV) is a subgroup of retroviruses. The lentiviral vector system transformed from HIV-1 has become the main choice of researchers in recent years due to its high efficiency and stable gene transfer efficiency. Because LV can infect both dividing cells and non-dividing cells, and the target gene integrated into the host genome carried by LV has strong resistance to transcriptional silencing, so that the target gene can be expressed in the host cell. long-term and stable expression. At the same time, the process of infecting target cells with lentivirus is similar to the process of infecting cells with natural HIV virus, but lentivirus cannot replicate itself and has high biological safety. In summary, compared with other retroviral vectors, LV has higher gene transduction efficienc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/10C12N15/867A61P35/00
CPCA61P35/00C12N5/0636C12N15/86C12N2510/00C12N2740/15043
Inventor 焦顺昌张嵘张天赋周子珊宋玉洁陈小彬解佳森
Owner BEIJING DCTY BIOTECH CO LTD
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