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Culturing method of euglena gracilis klebs

A culturing method and a technique for Euglena slenderness, applied in the field of culturing Euglena slenderness, can solve the problems of low cell density and low chlorophyll content of Euglena slenderness and the like

Inactive Publication Date: 2019-08-09
XIAMEN CANCO BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The euglena obtained by the current culture method has a low cell density and low chlorophyll content in the cells

Method used

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  • Culturing method of euglena gracilis klebs
  • Culturing method of euglena gracilis klebs
  • Culturing method of euglena gracilis klebs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Euglena slender: sourced from the Freshwater Algae Species Bank of the Chinese Academy of Sciences, numbered FACHB-745.

[0034] Prepare the AF-6 culture solution according to the components in Table 1 and Table 2, and adjust the pH value to 6.6.

[0035] 1) Inoculate Euglena gracilis in the AF-6 culture medium, and cultivate according to 10% inoculation amount, the culture temperature is 24°C, and the culture light condition is 60 μmol·m -2 ·s -1 ; Light to dark time ratio 12L:12D; Light source: incandescent lamp.

[0036] 2) After culturing for 1 day, add 200 μg / mL penicillin to the algae liquid in the logarithmic growth phase to continue culturing for 8 days, harvest Euglena gracilis, and measure algae cell density and chlorophyll a content every day.

[0037] Measure the cell density of Euglena gracilis with a hemocytometer; measure the content of chlorophyll a with the acetone method,

[0038] Protein content was determined by Coomassie brilliant blue method.

...

Embodiment 2

[0044] Euglena slender: sourced from the Freshwater Algae Species Bank of the Chinese Academy of Sciences, numbered FACHB-745.

[0045] Prepare the AF-6 culture solution according to the components in Table 1 and Table 2, and adjust the pH value to 6.6.

[0046] 3) Inoculate Euglena gracilis in the AF-6 culture solution, and cultivate according to 10% inoculation amount, the culture temperature is 24°C, and the culture light condition is 65 μmol·m -2 ·s -1 ; Light to dark time ratio 12L:12D; Light source: incandescent lamp.

[0047] 4) After culturing for 2 days, add 100 μg / mL penicillin to the algae liquid in the logarithmic growth phase to continue culturing for 8 days, harvest Euglena gracilis, and measure the algal cell density and chlorophyll a content every day.

[0048] The cell density of Euglena gracilis was measured by hemocytometer; the content of chlorophyll a was determined by acetone method.

[0049] The results are shown in Table 4.

[0050] Table 4 Cell den...

Embodiment 3

[0053] Euglena slender: sourced from the Freshwater Algae Species Bank of the Chinese Academy of Sciences, numbered FACHB-745.

[0054] Prepare the AF-6 culture solution according to the components in Table 1 and Table 2, and adjust the pH value to 6.6.

[0055] 5) Inoculate Euglena gracilis in the AF-6 culture medium, and cultivate according to 10% inoculation amount, the culture temperature is 25°C, and the culture light condition is 55 μmol·m -2 ·s -1 ; Light to dark time ratio 12L:12D; light source incandescent lamp.

[0056] 6) After culturing for 2 days, add 70 μg / mL penicillin to the algae liquid in the logarithmic growth phase to continue culturing for 8 days, harvest Euglena gracilis, and measure algae cell density and chlorophyll a content every day.

[0057] The cell density of Euglena gracilis was measured by hemocytometer; the content of chlorophyll a was determined by acetone method.

[0058] The results are shown in Table 5.

[0059] Table 5 Cell density and c...

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Abstract

The invention provides a culturing method of euglena gracilis klebs and belongs to the technical field of algae culturing. The method comprises the following steps of 1, culturing euglena gracilis klebs in an euglena culture solution until a logarithmic growth phase to obtain an algae solution in the logarithmic growth phase; 2, adding a penicillin solution with the final concentration of 75-250 micrograms / milliliter into the algae solution in the logarithmic growth phase for continuous culturing for 3-8 days. According to the method, the penicillin is added into the algae solution in the logarithmic growth phase, so that the cell density of euglena gracilis klebs and the content of chlorophyll a in the cells are obviously increased. According to the records of the embodiment, the cell density of euglena gracilis klebs cultured by means of the method can reach 11.33*105 cells / milliliter, and the initially measured cell density is 5*105 cells / milliliter. The content of chlorophyll a inthe cells is 18 milligrams / liter, and the initially measured content is 4.9 milligrams / liter.

Description

technical field [0001] The invention belongs to the technical field of algae cultivation, and in particular relates to a method for cultivating Euglena gracilis. Background technique [0002] Euglena is the botanical name of the ancient protozoan Euglena, an ancient single-celled organism. Euglena is a microcellular algae with both plant and animal characteristics, capable of autotrophic and heterotrophic survival, and can grow and reproduce in fresh water, sea water and mixed water. As an important resource microalgae, euglena is rich in nutrients such as amino acids, unsaturated fatty acids, vitamins and phyllose, and euglena has no cell wall and bare cytoplasm, and its nutrients are easier to be absorbed by others than other algae. Absorption by human body and aquatic animals. [0003] Euglena can carry out autotrophy and heterotrophy. In order to promote the growth of Euglena, there are usually ways to adjust the light intensity, suitable light time, suitable nitrogen ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 姜宗然马希望
Owner XIAMEN CANCO BIOTECH CO LTD
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