Method for predicting health and immunity levels of introduced milk cows based on intestinal flora

A technology for intestinal flora and immunity, applied in the field of biotechnology, can solve the problems of long sequencing time, high sequencing cost, and complicated calculation, and achieve the effects of low cost, convenient sampling, and fast analysis speed.

Inactive Publication Date: 2019-08-20
DALIAN UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

In addition, the high cost of sequencing, long sequencing time, and complex calculations of metagenomic detection methods make it difficult to apply this method in business

Method used

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  • Method for predicting health and immunity levels of introduced milk cows based on intestinal flora
  • Method for predicting health and immunity levels of introduced milk cows based on intestinal flora
  • Method for predicting health and immunity levels of introduced milk cows based on intestinal flora

Examples

Experimental program
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Embodiment 1

[0028] Step 1. Sample collection: After the farm makes a test request, it will receive relevant fecal sample collectors, one for each cow, to collect fecal samples from 3 cows that have been imported for less than half a year, numbered xzjs1, xzjs2, xzjs3, 3 respectively The feces samples of the first cows imported for more than half a year are numbered syjs1, syjs2, and syjs3 respectively, and the information on the month of introduction of each cow is recorded in detail. The feces are put into the collection tube containing the preservation solution and sent back to the sample center by courier. All samples were collected at room temperature and transported on dry ice.

[0029] Step 2. Establish database and variable region sequencing: use kit DNA purification technology to extract bacterial DNA in feces and establish database;

[0030] Sequencing fragments: The sequencing platform Illumima MiSeq sequencer sequenced the 16S variable region to obtain the original off-machine ...

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Abstract

The invention relates to the technical field of biotechnology, in particular to a method for extracting and analyzing the DNA of introduced milk cow feces samples to predict the health and immunity levels of the introduced milk cows. The method comprises the processes such as sample selection, 16S amplicon sequencing, sequencing data analysis, intestinal flora diversity and abundance analysis, user report push and the like. According to the method, a monitoring index of intestinal flora diversity and abundance analysis is linked with the health and immunity levels of the introduced milk cows,after experiment studies and data analysis, fresh introduced (fewer than half a year) and early introduced (more than half a year) milk cow feces samples are collected, a hypervariable region is amplified, and the method for predicting the health and immunity levels of the introduced milk cows is realized. The samples are easy to collect, the analysis speed is rapid, the cost is low, and the assessment results are consistent with the existing studies, making the method for predicting the health and immunity levels of the introduced milk cows based on the intestinal flora possible.

Description

technical field [0001] The invention relates to the technical field of biotechnology, in particular to a method for extracting and analyzing the DNA of feces samples from imported cows to predict their health and immunity levels. Background technique [0002] The normal intestinal flora is critical to maintaining the growth and health of cattle, and can help the host resist pathogenic microorganisms and regulate immune responses. The composition of intestinal flora is affected by many factors, including changes in the living environment. Once the balance is broken, the host body may mistakenly attack its own cells and tissues, leading to an increase in the prevalence of immune diseases and affecting the health of the host. If it is not found in time or treated properly, it may even lead to the death of dairy cows, causing huge economic losses in the breeding industry. In recent years, intestinal flora has become a research hotspot. [0003] At present, there are two main m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/689C12Q1/6869C12Q1/04
CPCC12Q1/689C12Q1/6869C12Q2535/122
Inventor 张庆芳杨超姜南迟乃玉于爽
Owner DALIAN UNIV
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