Novel multilayer adherent cell culture vessel structure with two breathable sides

Abstract

The invention relates to the field of culture vessels, in particular to a novel multilayer adherent cell culture vessel structure with two breathable sides. The structure comprises a multilayer adherent cell culture vessel main body, groups of multilayer dividing plates and bayonet covers, wherein mounting openings are formed in the portions of the side, close to a base surface and a back surface,of the upper end of the multilayer adherent cell culture vessel main body respectively; the bayonet covers are fixedly mounted at the upper ends of the mounting openings, and square bayonets are formed in the centers of the upper ends of the bayonet covers; the multilayer dividing plates are arranged in the multilayer adherent cell culture vessel main body at equal intervals from top to bottom; cell inlet through holes are formed in the portions, close to the mounting openings, of the multilayer dividing plates; positioning clamping grooves are formed in the portions, close to the multiplayerdividing plates, of the multilayer adherent cell culture vessel main body, and breathable films are arranged on the base surface and back surface of the multilayer adherent cell culture vessel main body respectively. Due to the application of the breathable films in the structure, a vessel can be used for full-liquid culture, the layer height is reduced, the culture area is greatly increased at the same height, and the spatial utilization rate is increased.

Description

Technical field [0001] The invention relates to the field of culture containers, in particular to a new type of air-permeable multilayer adherent cell culture container structure on both sides. Background technique [0002] Anchorage-dependent cells must be attached to the wall of the culture vessel when they are cultured. Once the cells are attached, they spread quickly, then begin mitosis, and quickly enter the logarithmic growth phase. Generally, the culture surface is covered and compacted after a few days. Cell monolayer. [0003] Disadvantages of adherent culture: compared with suspension culture, it is more difficult to expand the culture, the investment is large, and the area is large. As the number of layers increases, there must be a certain height between the layers to meet the ventilation requirements. The method of full liquid culture can also achieve a certain amount of air permeability, which can greatly shorten the height between layers, save space, and increase th...

AI Technical Summary

Problems solved by technology

[0003] Disadvantages of adherent culture: Compared with the suspension culture method, it is more difficult to expand the culture, the investment is large, and the floor space is large. As the number of layers of multi-layer adherent cell culture containers increases, there must be a certain height between layers to meet the ventilation requirements. If there is The method of full liquid culture can also achieve a certain amount of air flow, which can greatly shorten the height between layers, save space, and increase the culture area. The air permeability inside the container needs to meet the needs of cells. Therefore, a new type of air permeability on both sides is needed. The layer-adhered cell culture container structure improves the above problems

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