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A HPLC fingerprint of Zhideke Granules and its quality analysis method

A fingerprint and particle technology, applied in the direction of analyzing materials, measuring devices, material separation, etc., can solve the problems of limited research, and achieve the effect of good reproducibility, high precision and good stability

Active Publication Date: 2022-03-04
广西中医药大学附属瑞康医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no research on the fingerprints of Zhideke granules, and the current evaluation of fingerprints mostly uses similarity analysis as the basic evaluation method. It has a certain impact on the accuracy of the similarity, but the research on related aspects is limited

Method used

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  • A HPLC fingerprint of Zhideke Granules and its quality analysis method
  • A HPLC fingerprint of Zhideke Granules and its quality analysis method
  • A HPLC fingerprint of Zhideke Granules and its quality analysis method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A HPLC fingerprint of Zhideke granules, the construction method of which specifically comprises the following steps:

[0030] (1): Make the Zhideke Granules into a sample to be tested according to the following extraction method, and set aside; the extraction method is: take the Zhideke Granules, add pure water precisely, put it in an ultrasonic instrument for quantitative extraction for 25 minutes, and take out Let cool to room temperature, use pure water to make up for weight loss, at 12000r·min -1 Centrifuge for 10 minutes, and filter the supernatant with a 0.45 μm microporous membrane, which is the sample to be tested for later use; the mass ratio of Zhideke granules to pure water is 2.0:9;

[0031] (2): Accurately weigh the reference substances baicalin, irisflavin, wogonin, and saikosaponin d, respectively, and add methanol to prepare a mixed reference solution. The mixed reference solution includes a concentration of 0.371 mg·mL -1 Baicalin, the concentration is...

Embodiment 2

[0035] A HPLC fingerprint of Zhideke granules, the construction method of which specifically comprises the following steps:

[0036](1): Make the Zhideke Granules into the sample to be tested according to the following extraction method, and set aside; the extraction method is: take the Zhideke Granules, add pure water precisely, put it in an ultrasonic instrument for quantitative extraction for 30 minutes, and take out Let cool to room temperature, use pure water to make up for weight loss, at 13000r·min -1 Centrifuge for 12 minutes, and filter the supernatant with a 0.45 μm microporous membrane, which is the sample to be tested for later use; the mass ratio of Zhideke granules to pure water is 2.0:10;

[0037] (2): Accurately weigh the reference substances baicalin, irisflavin, wogonin, and saikosaponin d respectively, and add methanol to prepare a mixed reference solution. The mixed reference solution includes a concentration of 0.3 mg·mL -1 Baicalin, the concentration is ...

Embodiment 3

[0041] A HPLC fingerprint of Zhideke granules, the construction method of which specifically comprises the following steps:

[0042] (1): Make the Zhideke granule into a sample to be tested according to the following extraction method, and set it aside; the extraction method is: take the Zhideke granule, add pure water precisely, put it in an ultrasonic instrument for quantitative extraction for 28 minutes, and take out Let cool to room temperature, use pure water to make up for weight loss, at 12500r·min -1 Centrifuge for 11 minutes, and filter the supernatant with a 0.45 μm microporous membrane, which is the sample to be tested for use; the mass ratio of Zhideke granules to pure water is 2.0:9.5;

[0043] (2): Accurately weigh the reference substances baicalin, irisflavin, wogonin, and saikosaponin d, respectively, and add methanol to prepare a mixed reference solution. The mixed reference solution includes a concentration of 0.5 mg·mL -1 Baicalin, the concentration is 0.02...

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Abstract

The invention discloses an HPLC fingerprint of Zhideke granules and a quality analysis method thereof. The quality analysis method uses cluster analysis to analyze the fingerprints of Zhideke granule samples, and the shared results of multiple batches of samples are analyzed. The peak area of ​​the peak was normalized as a variable, imported into IBM SPSS Statistics 20.0 software, and the sum of squared deviation method was selected, and the Euclidean distance was used as the measurement standard of the sample to obtain a dendrogram. Classify Zhideke Granules and evaluate the quality of Zhideke Granules. It can more accurately evaluate the comprehensive quality score of Zhideke Granules, and provide a basis for establishing a sound quality standard for Zhideke Granules, with a view to establishing a quality control and evaluation system for Zhideke Granules.

Description

technical field [0001] The invention belongs to the technical field of drug analysis, and in particular relates to an HPLC fingerprint of Zhideke granules and a quality analysis method thereof. Background technique [0002] Zhideke Granules is an in-hospital preparation of Ruikang Hospital Affiliated to Guangxi University of Traditional Chinese Medicine. It is a traditional Chinese medicine compound preparation composed of ten medicinal materials such as asparagus leaves, shegan, bellflower, scutellaria baicalensis, and Bupleurum. effect. The research on the granules is still in its infancy, and so far the research on the overall quality control of Zhideke Granules has not been perfected. The current quality control method only uses simple TLC qualitative identification and the two chemical components of baicalin and irisin to characterize the quality of the preparation. The standard is relatively low, and it is impossible to characterize the chemical substance group of the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/06G01N30/74G01N30/86
CPCG01N30/06G01N30/74G01N30/8686
Inventor 梁洁周昱杉孙正伊唐云丽陈俊祁静陈日兰
Owner 广西中医药大学附属瑞康医院
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