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Application of extract of cynanchum stauntonii in preparation of drugs for relaxing airway smooth muscle

A technology of airway smooth muscle and willow leaf white, which is applied in the field of medicine

Inactive Publication Date: 2019-10-08
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no relevant reports at home and abroad on the research on the relaxation of airway smooth muscle by Liuyebaiqian and its extracts.

Method used

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  • Application of extract of cynanchum stauntonii in preparation of drugs for relaxing airway smooth muscle
  • Application of extract of cynanchum stauntonii in preparation of drugs for relaxing airway smooth muscle
  • Application of extract of cynanchum stauntonii in preparation of drugs for relaxing airway smooth muscle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The preparation of embodiment 1 willow leaf white extract

[0022] Willow Ye Baiqian was purchased from Bozhou, Anhui. Grind the dried willow leaves Baiqian, cold soak with 80% ethanol to obtain the extract, evaporate the extract to dryness under reduced pressure with a rotary evaporator to obtain extract, add a certain volume of water to dissolve, first extract with petroleum ether, and then The separated water phase is further added with ethyl acetate for extraction, and the extracted ethyl acetate phase is evaporated to dryness under reduced pressure and concentrated into an extract, which is the extract of the ethyl acetate part of the willow leaf. The ethyl acetate extract of willow leaf white front was dissolved in DMSO for the biological activity test.

Embodiment 2

[0023] Example 2 Preconstriction of the airway smooth muscle induced by diastolic high potassium extract

[0024] 1. In vitro isolation of mouse trachea:

[0025] Male adult BALB / c mice were sacrificed by cervical dislocation, and the abdomen of the mice was turned upward. The head and limbs were fixed on the dissecting board, sprayed with 75% alcohol, the hair was wetted to prevent flying hairs and disinfected, and then Use sterilized scissors to cut open the thorax, expose the trachea and lung tissue, remove a whole section of tissue from the Adam's apple to the lung and remove the heart, soak the removed tissue in pre-cooled PSS solution immediately, and then Perform further trimming on a dissecting dish. Under the stereoscope, the esophagus was first subtracted with Venus scissors, and then the connective tissue and adipose tissue around the trachea were carefully removed.

[0026] 2. The tension detection method of mouse airway smooth muscle:

[0027] Cut the trachea o...

Embodiment 3

[0034] Example 3 Preconstriction of airway smooth muscle induced by diastolic acetylcholine induced by the extract of Leipzig baicalensis

[0035] The remaining steps were the same as in Example 2, except that 80 mM high potassium solution (H-K) was replaced with 100 μM acetylcholine (ACh) solution for pre-stimulation after equilibration.

[0036] Effects of acetylcholine on airway smooth muscle contraction image 3 , The isolated mouse tracheal rings contracted continuously and stably in 100 μM ACh solution. When EAEC was added, the tension of the tracheal rings gradually decreased, and the tension of the tracheal rings gradually returned to the pre-contraction level with the increase of the concentration of EAEC. It shows that EAEC can completely relax acetylcholine and cause contraction of airway smooth muscle, and the drug effect is dose-dependent.

[0037] The relaxation rate results under different concentrations of willow leaf white former ethyl acetate extract are sho...

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PUM

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Abstract

The invention discloses application of an extract of cynanchum stauntonii in the preparation of drugs for relaxing the airway smooth muscle. The tension of the isolated tracheal circular muscle of anexperimental mouse is detected, the extract of the cynanchum stauntonii relaxes pre-contraction of the airway smooth muscle induced by acetyl choline and potassium, and it is found that the extract ofthe cynanchum stauntonii is a highly effective drug for relaxing the airway smooth muscle; and it is found that ethyl acetate extract (EAEC) of the cynanchum stauntonii can block other channels except for a VDCCs channel to participate in the relaxation of tracheal smooth muscle; and the VDCCs channel is excluded, the ethyl acetate extract of cynanchum stauntonii can block other channels except for VDCCs and NSCCs to participate the relaxation of the airway smooth muscle.

Description

technical field [0001] The invention belongs to the technical field of medicines, and in particular relates to the application of the extract of the basilica extract in the preparation of medicines for relaxing airway smooth muscle. Background technique [0002] Asthma, also known as bronchial asthma, is a common multiple chronic respiratory disease that threatens the lives and health of hundreds of millions of people around the world. Symptom attacks are often accompanied by recurrent episodes of wheezing, shortness of breath, chest tightness, and coughing. Asthma is not easy to be completely cured, and it can also cause many diseases. Periodic attacks of asthma can seriously affect patients' daily life, leading to symptoms such as insomnia, fatigue, and decreased activity. The clinical symptoms of asthma patients vary from person to person, and the main clinical symptoms include airway inflammation, airway remodeling and airway hyperresponsiveness. [0003] Airway hyper...

Claims

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Application Information

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IPC IPC(8): A61K36/27A61P11/06A61P11/08
CPCA61K36/27A61K2236/333A61K2236/55A61P11/06A61P11/08
Inventor 赵平何婧雯
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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