Composite amplification kit for simultaneously detecting 39 Y chromosome STR loci

Abstract

The invention discloses a composite amplification kit for simultaneously detecting 39 human Y chromosome STR loci. 39 Y chromosome loci are simultaneously amplified in a composite amplification reaction system. The composite amplification kit comprises 26 single-copy loci, 3 double-copy loci, one three-copy loci and one four-copy loci. The 39 loci are divided into five groups through a loca arrangement mode which is unique in design and a specific primer sequence, and each group is labeled with a different fluorescent label. The kit has high resolution and high individual identification amongmale samples.

Description

technical field

[0001] The invention belongs to the technical field of DNA identification, in particular to a composite amplification kit for simultaneously detecting 39 STR loci of human Y chromosome. Background technique

[0002] The use of STR markers has become a standard tool in analyzing DNA found at crime scenes. In most cases, autosomal STR markers are utilized, in part because of their high levels of polymorphism in most populations. For example, in the United States, the standard 13 CODIS loci used to establish suspect DNA databases are autosomal STR markers. In many cases of mixed blood from male and female contributors, particularly in rape cases, forensic investigators must analyze genetic markers present on the Y chromosome to identify the male component, usually the perpetrator. This is because, in such cases, autosomal STR markers are not informative due to, for example, spectral overlap between female victim DNA and male offender DNA. Although techniques ...

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