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Composite amplification kit for simultaneously detecting 39 Y chromosome STR loci

A Y chromosome and compound amplification technology, applied in the field of compound amplification kits, can solve problems such as narrowing the family range, and achieve the effects of high compatibility and high individual recognition rate

Inactive Publication Date: 2019-10-25
武汉瑞博科创生物技术有限公司 +1
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Problems solved by technology

[0004] Although there are many kinds of commercialized Y-STR kits at present, the mainstream kits include YfilerPlus, PowerPLexY23, GoldeneyeY26, STRtyper27Y, AGCUDatabaseY24, but Yfiler with the most loci TM The Platinum PCR amplification kit can only compound and amplify 38 Y-STR loci, which cannot meet the requirements of further narrowing down the range of families in actual work

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  • Composite amplification kit for simultaneously detecting 39 Y chromosome STR loci
  • Composite amplification kit for simultaneously detecting 39 Y chromosome STR loci
  • Composite amplification kit for simultaneously detecting 39 Y chromosome STR loci

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Embodiment Construction

[0026] The present invention is further described below by way of examples. The following examples are for illustrative purposes only and do not limit the scope of the invention.

[0027] Example Compound amplification of 39 loci and analysis of their genotypes

[0028] The standard DNA 007 was purchased, and the template DNA was directly amplified. Amplification reactions were performed on an ABI 9700 thermal cycler, electrophoresis and detection were performed on an ABI 3130xl genetic analyzer, and GeneMapper ID v3.2 software was used for data analysis. The reagents and materials used in the present invention, such as allelic ladders, are conventional materials commonly used by those skilled in the art.

[0029] The 39 STR loci of human Y chromosome described in the present invention are: DYS426, DYS593, DYS630, YPENTA1, DYS722, DYS617, YPENTA2, DYS443, Y-GATA-A10, DYS561, DYF404S1, DYS464, DYS713, DYS446, DYS607, DYS708 , DYS622, DYS707, DYS520, DYS434, DYS505, DYS709, D...

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Abstract

The invention discloses a composite amplification kit for simultaneously detecting 39 human Y chromosome STR loci. 39 Y chromosome loci are simultaneously amplified in a composite amplification reaction system. The composite amplification kit comprises 26 single-copy loci, 3 double-copy loci, one three-copy loci and one four-copy loci. The 39 loci are divided into five groups through a loca arrangement mode which is unique in design and a specific primer sequence, and each group is labeled with a different fluorescent label. The kit has high resolution and high individual identification amongmale samples.

Description

technical field [0001] The invention belongs to the technical field of DNA identification, in particular to a composite amplification kit for simultaneously detecting 39 STR loci of human Y chromosome. Background technique [0002] The use of STR markers has become a standard tool in analyzing DNA found at crime scenes. In most cases, autosomal STR markers are utilized, in part because of their high levels of polymorphism in most populations. For example, in the United States, the standard 13 CODIS loci used to establish suspect DNA databases are autosomal STR markers. In many cases of mixed blood from male and female contributors, particularly in rape cases, forensic investigators must analyze genetic markers present on the Y chromosome to identify the male component, usually the perpetrator. This is because, in such cases, autosomal STR markers are not informative due to, for example, spectral overlap between female victim DNA and male offender DNA. Although techniques ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 罗冰科王新杰钟巧梅何悦
Owner 武汉瑞博科创生物技术有限公司
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