Method for detecting Zika virus by using RPA technology and special complete set of reagent for method

Abstract

The invention discloses a method for detecting Zika virus by using an RPA technology and a special complete set of reagent for the method. The complete set of reagent consists of a primer pair X and aprobe X. The primer pair X consists of a primer F as shown in a sequence 3 in a sequence table and a primer R as shown in a sequence 2 in the sequence table. The probe X comprises a DNA fragment A, tetrahydrofuran and a DNA fragment B in sequence from 5' to 3'. The DNA fragment A and the DNA fragment B are respectively single-stranded DNA molecules as shown in a sequence 4 and a sequence 5 in thesequence table. Experiments prove that the complete set of reagent provided by the invention can detect Zika virus by performing RPA, and has good specificity and high sensitivity. The reagent has great practical value.

Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a method for detecting Zika virus by using RPA technology and a set of special reagents thereof. Background technique

[0002] Zika virus (ZIKV) belongs to the Flaviviridae family and is transmitted by mosquito bites. Zika virus was first discovered in the blood of rhesus monkeys in Uganda's Zika forest in 1947. Symptoms of Zika virus infection are usually mild, and Zika virus infection has been ignored for a long time. Since 2015, Zika virus has caused a worldwide epidemic, with more than 1 million cases of Zika virus infection in Brazil. In China, several cases of Zika virus infection have also been found. Research has shown that Zika virus infection can cause microcephaly in newborns and has also been linked to other birth defects. The development of rapid detection reagents for Zika virus is crucial to controlling the spread of Zika virus and adopting effective pr...