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SNP markers related to growth traits of black-bone chicken and their application

A technology of marking and black-bone chicken, which is applied in the determination/inspection of microorganisms, recombinant DNA technology, biochemical equipment and methods, etc., can solve the problems of high breeding cost, inaccurate selection, long breeding process, etc.

Active Publication Date: 2022-04-15
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the interference of environmental factors, traditional phenotypic selection has the problem of inaccurate selection
Moreover, the traditional breeding methods all need to measure the phenotypic value of the individual, the breeding process is long, and the breeding cost is high

Method used

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  • SNP markers related to growth traits of black-bone chicken and their application
  • SNP markers related to growth traits of black-bone chicken and their application
  • SNP markers related to growth traits of black-bone chicken and their application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1. Verification of the correlation between the SNP marker rs317682933 and the body weight and feed intake of Lueyang black-bone chicken

[0045] 1. Test animals

[0046] Lueyang black-bone chickens are randomly selected from three generations of Lueyang black-bone egg breeding core group bred by Lueyang Longjia Agricultural Science and Technology Development Co., Ltd. All Lueyang black-bone chickens are hatched in the same batch and raised in the same chicken house. 40pcs / m 2Cage brooding; brooding temperature is 33-35°C in the first week, then lowered by 2-3°C every week, and removed from the temperature in the fourth week; the light time is 22h in the first week, and then reduced by 1-1.5h every week, using Huaqin 911A egg chicks were fed with compound feed. In 60 days, they were transferred to the laying hen house and raised in individual single cages.

[0047] 2. Determination of body weight and material consumption

[0048] Measure the body weight of bl...

Embodiment 2

[0081] Embodiment 2, the comparison of the breeding effect of traditional phenotype selection and marker-assisted selection

[0082] 1. Test animals

[0083] 500 hens and 200 roosters were randomly selected from the second generation of egg-line breeding core group raised by Lueyang Longjia Agricultural Science and Technology Development Co., Ltd. to form a traditional phenotype selection breeding group, and another 500 hens and 200 roosters were selected SNP marker-assisted selection breeding population.

[0084] 2. Test plan

[0085] Traditional phenotype selection is based on 12-week-old body weight, and individual phenotype selection is adopted. The top 5% of cocks are sorted by 12-week-old body weight, and the top 5% of hens are reserved for 25%. The marker-assisted breeding method not only considers the body weight at 12 weeks, but also considers the genotype of the rs317682933 locus, and the proportion of reserved seeds is the same as that of the traditional breeding ...

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Abstract

The invention discloses a SNP marker related to the growth traits of black-bone chicken and its application. The present invention firstly provides the application of the polymorphism or genotype detection material of SNP marker rs317682933 in the identification or auxiliary identification of chicken body weight. Nucleic acid, its alleles are C and T. The present invention further provides a method for identifying or assisting in identifying the body weight of chickens. The present invention finds that the genotype of the SNP marker rs317682933 in the chicken genome is CC and the body weight is larger than that of the genotype CT, and the correlation between the marker and the body weight gradually increases with the age of the chicken. Therefore, the use of SNP marker rs317682933 in the growth performance breeding of Lueyang black-bone chicken can improve the accuracy of selection, increase the selection response and shorten the breeding process.

Description

technical field [0001] The invention relates to the field of molecular marker assisted breeding, in particular to a SNP marker related to the growth traits of black-bone chicken and its application. Background technique [0002] Lueyang black-bone chicken is a unique poultry breed in Shaanxi Province, and it is also a larger black-bone chicken among the existing black-bone chickens in China. The variety has tender meat, delicious taste, rich nutrition and health care functions. Poultry products are deeply loved by consumers, and the development of the black-bone chicken industry has a good market prospect. However, because Lueyang black-bone chicken has not been systematically selected, this variety also has the disadvantages of slow growth, high feed-to-meat ratio, and poor uniformity. The average body weight of Lueyang black-bone chickens at 12 weeks old is 1.38±0.19 kg, and the average weight of hens is 1.04±0.13 kg; the feed-to-meat ratio of roosters at 12 weeks is 7.0...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124
Inventor 王哲鹏周雯馨
Owner NORTHWEST A & F UNIV
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