Color digital transmission microscope color correction method

A transmission microscope and color correction technology, applied in the direction of color/spectral characteristic measurement, etc., can solve problems such as image processing, analysis of negative effects, affecting visual effects, rough color correction methods, etc., to achieve accurate identification and positioning, and improve color accuracy , Improve the effect of recognition

Active Publication Date: 2020-01-17
NINGBO YONGXIN OPTICS
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AI Technical Summary

Problems solved by technology

However, the processing software also has very high requirements for the image color quality of the digital microscope imaging system. If the image color quality is not good, it will not only affect the visual effect, but also have a great negative impact on the post-processing and analysis of the image. Color Correction of Digital Transmission Microscope Images
Most of the current color correction solutions use the white balance algorithm, but the white balance algorithm is only a rough color correction method, and cannot achieve accurate color correction of the digital microscope imaging system

Method used

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  • Color digital transmission microscope color correction method
  • Color digital transmission microscope color correction method
  • Color digital transmission microscope color correction method

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Embodiment Construction

[0029] The present invention will be described in further detail below in conjunction with the embodiments of the drawings.

[0030] Taking a biological microscope as an example, the method of color digital transmission microscope correction based on the micro transmission color card is described. The micro transmission color card adopts a transmission color card containing 45 color blocks, and the size of the color card is 3mm×4mm.

[0031] The method for correcting the color of a color digital transmission microscope based on a micro transmission color card in this embodiment specifically includes the following steps:

[0032] (1) Turn on the illuminating light source of the color digital transmission microscope, warm up for a period of time, place the micro transmission color card under the eyepiece of the stage, take the micro transmission color card, and obtain the RGB value of all the color blocks in the color card image, which is recorded as P is a matrix of 45 rows and 3 colu...

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Abstract

The invention discloses a color digital transmission microscope color correction method. A micro transmission color card is used for correcting colors of microscopic images collected by a color digital transmission microscope, since an illumination light source of the digital microscope is usually fixed, chroma characterization of a digital camera under fixed conditions is particularly suitable for color correction of the digital microscope, therefore, the color blocks in the micro transmission color card are used as training samples to establish a positive chroma characterization model of thecolor digital transmission microscope, and the model can be used for realizing accurate color correction of a shooting target; and the color accuracy of the color digital transmission microscope is improved, the method is consistent with the color perception of human eyes, the effect that what you see is what you get is achieved, the recognition degree of a target object in microscopic inspectionis significantly improved, and the accurate identification and positioning of the target object are realized.

Description

Technical field [0001] The invention relates to a color correction method for a microscope, in particular to a color correction method for a color digital transmission microscope. Background technique [0002] In the color digital imaging system, people have put forward higher and higher requirements for the color quality of the image. The color formation of the digital microscope imaging system has its particularity. On the one hand, it must conform to the sensory characteristics of the human eye, and on the other hand, it must reflect the original color of the object as truly as possible. A large part of the existing color digital transmission microscopes are used in medical detection, and many pathological features are distinguished by the color changes of cells, which require relatively high color authenticity. Microscopic image processing software is an integral part of the digital microscopic imaging system. It converts the data collected by the image sensor into a color i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/25
CPCG01N21/25
Inventor 毛磊徐鹏张克奇张琦邱元芳
Owner NINGBO YONGXIN OPTICS
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