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Method for rooting in tissue culture seedling bottle of early-maturing walnut

A technique for tissue culture of seedlings and walnuts, which is applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of easily carrying viruses, slow rooting speed, and low propagation coefficient of cutting propagation, so as to shorten the induction time for rooting, The effect of reducing rooting rate and strong root system

Inactive Publication Date: 2020-02-04
TARIM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Early-fruited walnuts are favored by fruit farmers due to their early results, high yields, and high economic benefits. At present, various processed walnut products are in short supply in the international market. However, due to the rapid expansion of its cultivation area, conventional cutting propagation can no longer satisfy the market demand for seedlings. In addition, the propagation coefficient of cutting propagation is low, the rooting speed is slow, and it is easy to carry viruses, which hinders the expansion of early-fruiting walnut planting area

Method used

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  • Method for rooting in tissue culture seedling bottle of early-maturing walnut

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Effect test

Embodiment 1

[0018] 1, the early-fruiting walnut tissue culture seedling rooting strong seedling medium of the present embodiment comprises:

[0019] Early fruiting walnut tissue culture seedling rooting and strong seedling medium I: 5mg / L vitamin C, 8mg / LIAA, 30g / L sucrose and 6g / L agar in the basic medium, the pH value is 5.5-6.0;

[0020] Early fruiting walnut tissue culture seedling rooting and strong seedling medium II: basic medium, 0.7mg / L vitamin C, 0.8mg / L IAA, 15g / L sucrose and 4g / L agar.

[0021] 2, the method for suppressing the browning of the explant of the exotic walnut of the present embodiment comprises:

[0022] Step 1, the early fruiting walnut tissue culture seedling is transferred into described early fruiting walnut tissue culture planting rooting strong seedling medium I earlier and carries out dark culture (dark culture time is 24~26h, and temperature is 23~27 ℃), then Placed in early fruit walnut tissue culture seedling rooting and strong seedling medium II, light...

Embodiment 2

[0025] 1, the early-fruiting walnut tissue culture seedling rooting strong seedling medium of the present embodiment comprises:

[0026] Early fruiting walnut tissue culture seedling rooting and strong seedling medium I: 4mg / L vitamin C, 5mg / LIAA, 30g / L sucrose and 6g / L agar in the basic medium, the pH value is 5.5-6.0;

[0027] Early fruiting walnut tissue culture seedling rooting and strong seedling medium II: basic medium, 0.6mg / L vitamin C, 0.5mg / L IAA, 15g / L sucrose and 4g / L agar.

[0028] 2, the method for suppressing the browning of the explant of the exotic walnut of the present embodiment comprises:

[0029] Step 1, the early fruiting walnut tissue culture seedling is transferred into described early fruiting walnut tissue culture planting rooting strong seedling medium I earlier and carries out dark culture (dark culture time is 24~26h, and temperature is 23~27 ℃), then Placed in early fruit walnut tissue culture seedling rooting and strong seedling medium II, light...

Embodiment 3

[0032] 1, the early-fruiting walnut tissue culture seedling rooting strong seedling medium of the present embodiment comprises:

[0033] Early fruiting walnut tissue culture seedling rooting and strong seedling medium I: 6mg / L vitamin C, 10mg / LIAA, 30g / L sucrose and 6g / L agar in the basic medium, the pH value is 5.5-6.0;

[0034] Early fruiting walnut tissue culture seedling rooting and strong seedling medium II: basic medium, 0.8mg / L vitamin C, 1mg / L IAA, 15g / L sucrose and 4g / L agar.

[0035] 2, the method for suppressing the browning of the explant of the exotic walnut of the present embodiment comprises:

[0036] Step 1, the early fruiting walnut tissue culture seedling is transferred into described early fruiting walnut tissue culture planting rooting strong seedling medium I earlier and carries out dark culture (dark culture time is 24~26h, and temperature is 23~27 ℃), then Placed in early fruit walnut tissue culture seedling rooting and strong seedling medium II, light ...

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Abstract

The invention provides a method for rooting in a tissue culture seedling bottle of early-maturing walnut, which includes the following steps: (1) transferring the tissue culture seedling of early-maturing walnut into a culture medium I for rooting and strengthening the tissue culture seedling of early-maturing walnut for dark culture, and then placing a culture medium II for rooting and strengthening the tissue culture seedling of early-maturing walnut for light culture and dark culture for alternate culture, wherein the medium I for rooting and strengthening the tissue culture seedling of early-maturing walnut comprises a basic medium, 4-6\mg / L vitamin C and 5-10mg / L IAA; the culture medium II for rooting and strengthening the tissue culture seedlings of early-maturing walnut comprises abasic culture medium, 0.6-0.8mg / L vitamin C and 0.5-1mg / L IAA; and (2) transplanting the rooted tissue culture seedlings of early-maturing walnut after hardening the seedlings. The method can obviously reduce the rooting rate of the exotic walnut explants, the rooting rate can reach 98.5%, and meanwhile, the induction rooting time can be shortened, root occurrence can be seen in 5-7 days, and eachplant has an average of 7-10 roots.

Description

technical field [0001] The invention relates to the technical field of asexual propagation of seedlings, in particular to a method for rooting early-fruiting walnut tissue culture seedlings in a bottle. Background technique [0002] Walnuts can be divided into early fruiting walnuts and late fruiting walnuts according to the early and late fruiting time. Early fruit walnut is a classification name of walnut. It usually refers to the varieties with faster fruiting speed of natural seedlings and grafted seedlings. Generally, the seedlings of early-fruiting varieties start to bear fruit in 2 to 3 years, and the grafted seedlings start to bear fruit in 1 to 2 years. Early-fruited walnuts are favored by fruit farmers due to their early results, high yields, and high economic benefits. At present, various processed walnut products are in short supply in the international market. However, due to the rapid expansion of its cultivation area, conventional cutting propagation can no ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 金强陈小飞冯一峰陈家力张锐王新建张宏刘多红
Owner TARIM UNIV
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