A kind of method of degrading corn stover
A corn stalk, magnetic nanoparticle technology, applied in biochemical equipment and methods, immobilized on or in an inorganic carrier, immobilized on/in an organic carrier, etc., can solve the problem that laccase is not widely used, laccase High production cost, poor stability and other problems, to achieve the effect of improving activity and stability, ensuring cellulase activity, and reducing inactivation rate
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Embodiment 1
[0045] A method for degrading corn stalks, comprising the following steps:
[0046] (1) After the corn stalks are mechanically crushed, pass through a 40-mesh sieve, and then dry at 105° C. for later use;
[0047] (2) Cu 2+ Modified Fe 3 O4-NH 2 -PEI magnetic nanoparticles were dissolved in NaAC-HAC buffer solution containing laccase, and treated with shaking at 150 rpm for 40min at 25°C; after the reaction, the prepared Cu 2+ Modified Fe 3 O4-NH 2 - PEI laccase magnetic nanoparticles and supernatant separation; ultrapure water for Cu 2+ Modified Fe 3 O4-NH 2 -PEI laccase magnetic nanoparticles were washed continuously until no laccase activity was detected in the detergent; the pH of NaAC-HAC buffer was 4.6, the concentration of laccase was 0.5mg / mL, Cu 2+ Modified Fe 3 O4-NH 2 -The mass volume ratio of PEI magnetic nanoparticles to NaAC-HAC buffer solution is 1mg: 10mL;
[0048] (3) Shake the immobilized laccase prepared in step (2), the corn stalks treated in ste...
Embodiment 2
[0052] A method for degrading corn stalks, comprising the following steps:
[0053] (1) After the corn stalks are mechanically crushed, pass through a 40-mesh sieve, and then dry at 105° C. for later use;
[0054] (2) Immobilize laccase on Cu 2+ Modified Fe 3 O4-NH 2 -On PEI magnetic nanoparticles, immobilized laccase was formed, specifically Cu 2+ Modified Fe 3 O4-NH 2 -PEI magnetic nanoparticles were dissolved in NaAC-HAC buffer solution containing laccase, and shaken at a rate of 150 rpm for 70 min at 25°C; after the reaction, the prepared Cu 2+ Modified Fe 3 O4-NH 2 - PEI laccase magnetic nanoparticles and supernatant separation; ultrapure water for Cu 2+ Modified Fe 3 O4-NH 2 -PEI laccase magnetic nanoparticles were washed continuously until no laccase activity was detected in the detergent; the pH of NaAC-HAC buffer was 4.6, the concentration of laccase was 4mg / mL, Cu 2+ Modified Fe 3 O4-NH 2 -The mass volume ratio of PEI magnetic nanoparticles to NaAC-HAC b...
Embodiment 3
[0059] A method for degrading corn stalks, comprising the following steps:
[0060] (1) After the corn stalks are mechanically crushed, pass through a 40-mesh sieve, and then dry at 105° C. for later use;
[0061] (2) Immobilize laccase on Cu 2+ Modified Fe 3 O4-NH 2 -On PEI magnetic nanoparticles, immobilized laccase was formed, specifically Cu 2+ Modified Fe 3 O4-NH 2 -PEI magnetic nanoparticles were dissolved in NaAC-HAC buffer solution containing laccase, and shaken at a rate of 150 rpm for 50 min at 25°C; after the reaction, the prepared Cu 2+ Modified Fe 3 O4-NH 2 - PEI laccase magnetic nanoparticles and supernatant separation; ultrapure water for Cu 2+ Modified Fe 3 O4-NH 2 - PEI laccase magnetic nanoparticles were washed continuously until no laccase activity was detected in the detergent; the pH of NaAC-HAC buffer was 4.6, the concentration of laccase was 20.5 mg / mL, Cu 2+ Modified Fe 3 O4-NH 2 -The mass volume ratio of PEI magnetic nanoparticles to NaAC-...
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