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Thymus-dependent lymphocyte antigen epitope peptides of primary liver cancer related antigens and application thereof

A primary liver cancer, antigen epitope technology, applied in the field of medical immunology and oncology, can solve the problems of limiting personalized detection and limiting the role of liver cancer-specific T cells

Active Publication Date: 2020-04-07
南京大户生物科技有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, there are still very few primary liver cancer-associated antigen T-cell epitopes that are presented by various HLA molecules and can stimulate the body to trigger T-cell responses, which limits the research on liver cancers carrying different HLA alleles. The detection of specific T cells in patients also limits the research on the role of liver cancer-specific T cells in the occurrence and development of liver cancer, and limits the personalized detection and analysis based on the individual differences of HLA genes and the differences of the liver cancer-related antigen peptides presented. Precision Immunotherapy

Method used

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  • Thymus-dependent lymphocyte antigen epitope peptides of primary liver cancer related antigens and application thereof
  • Thymus-dependent lymphocyte antigen epitope peptides of primary liver cancer related antigens and application thereof
  • Thymus-dependent lymphocyte antigen epitope peptides of primary liver cancer related antigens and application thereof

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Embodiment 1

[0079] Embodiment 1: HLA-A0201, HLA-A1101, HLA-A2402, HLA-A3101, HLA-A0206, HLA-A0207, HLA-A3303, HLA-A3001, HLA-A0203, HLA-A1102, HLA-A0301, HLA-A0101, HLA-A2601 molecule-restricted liver cancer-associated antigen T cell epitope, the sequence is screened and identified through the following steps:

[0080] 1Online virtual prediction of dominant T cell epitope peptides of three liver cancer-associated antigens restricted by 13 HLA-A molecules

[0081]Three liver cancer-associated antigenic proteins were selected: alpha-fetoprotein (AFP), glypican-3 (GPC3), and Golgi transmembrane glycoprotein 73 (GP73), and their amino acid sequences were obtained by searching the UniProt global protein resource database , select the most studied standard sequence; and then use six commonly used epitope peptide prediction databases such as SYFPEITHI, BIMAS, SVMHC, IEDB, NETMHC, and EPIJEN to make HLA-A0201, HLA-A1101, HLA-A2402, HLA-A3101, HLA- A0206, HLA-A0207, HLA-A3303, HLA-A3001, HLA-A020...

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Abstract

The invention belongs to the field of medical immunology and oncology and discloses thymus-dependent lymphocyte antigen epitope peptides of primary liver cancer related antigens and application thereof. The antigen epitope peptides are antigen peptides separately restricted by HLA-A0201, HLA-A1101, hLA-A2402, HLA-A3101, HLA-A0206, HLA-A0207, HLA-A3303, HLA-A3001, HLA-A0203, HLA-A1102, HLA-A0301, HLA-A0101 and HLA-A2601 molecules, and the varieties of the antigen epitope peptides sum to 110. The antigen epitope peptides can specifically bind to cytotoxic thymus-dependent lymphocytes so as to stimulate the activation, proliferation and differentiation of the thymus-dependent lymphocytes, so anti-tumor immune effect is exerted; the antigen peptides can be used for preparing therapeutic and preventive vaccines for liver cancer, can also be used for preparing detection reagents for detecting liver cancer-related antigen-specific cytotoxic thymus-dependent lymphocytes, and have potential application value in prevention, treatment and diagnosis of liver cancer.

Description

technical field [0001] The invention belongs to the fields of medical immunology and oncology, and particularly relates to three primary liver cancer-related antigen thymus-dependent lymphocyte antigen epitope peptides and applications thereof. Background technique [0002] Primary liver cancer is the fourth common malignant tumor and the third leading cause of tumor death in my country, which seriously threatens the life and health of our people. The pathological types of primary liver cancer are mainly hepatocellular carcinoma (HCC), accounting for 85% to 90%; there are also a few intrahepatic cholangiocarcinoma (ICC) and HCC-ICC mixed types, etc. The pathogenesis, biological behavior, molecular characteristics, clinical manifestations, histopathological morphology, treatment methods and prognosis are quite different among the three. [0003] In my country, high-risk groups for HCC mainly include hepatitis B virus (HBV) and / or hepatitis C virus (HCV) infection, long-term ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47A61K39/00A61P35/00G01N33/574G01N33/569
CPCA61P35/00G01N33/57438G01N33/56966C07K14/70539A61K39/001114G01N2333/70539Y02A50/30
Inventor 沈传来金萧萧
Owner 南京大户生物科技有限公司
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