A Realization Method of Phase Sensitive Spectroscopy of Spin-Coupling Network Based on Pure Chemical Shift

Abstract

The invention provides a spin coupling network phase sensitive spectrum implementation method based on pure chemical shift. The method mainly relates to spin excitation, selective repolymerization, pure chemical shift and phase-sensitive implementation. The method comprises: sending the uniformly stirred sample to be detected into a detection cavity of a nuclear magnetic spectrometer; performing tuning, shimming and field locking; measuring the non-selective pi / 2 pulse width; acquiring a hydrogen spectrum by utilizing the standard sequence to obtain spectrum peak information; importing a preset sequence and setting experimental parameters, and executing data sampling; carrying out pure chemical shift data processing to obtain a two-dimensional spectrum containing J coupling information andpure chemical shift; and carrying out two-dimensional phase-sensitive processing on the obtained spectrogram. The method has the beneficial effects that (1) a single spin coupling network two-dimensional spectrum with a pure absorption peak line type can be obtained by only one experiment; (2) the J coupling constant can be accurately measured.

Description

technical field [0001] The invention relates to a nuclear magnetic resonance (NMR, Nuclear Magnetic Resonance) spectroscopy detection method, in particular to a method for realizing phase-sensitive spectrum of a spin coupling network based on pure chemical shift. Background technique [0002] Chemical shift and J-coupling information provide crucial information at the molecular level for organic chemistry, structure analysis and compositional analysis of biomolecules. In the traditional method, although the 2D J-decomposition spectrum can separate the chemical shift and J-coupling information, the coupling constants of independent homonuclear spin pairs are still unmeasured, and there are dispersion components, and the phase of the spectrum is distorted. Although the new J decomposition spectrum proposed at present can measure the J coupling constant, most of them cannot overcome the problem of the dispersion component in the spectrum. In the form of the absolute value spect...

AI Technical Summary

Problems solved by technology

[0003] The main technical problem to be solved by the present invention is to provide a method for realizing phase-sensitive spectrum of spin-coupling network based on pure chemical shift, which can be applied to many spin-coupling measurement sequences based on pure chemical shift, and effectively solve the problem of absolute value display mode. , spectral line broadening, inaccurate measurement of J coupling constants, and the need for multiple experiments

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