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Application of ebracteolatain A in preparing drugs for treating breast cancer

A technology of euphorin and breast cancer, which is applied in the field of medicine and can solve problems such as researches that have not been reported yet

Inactive Publication Date: 2020-05-01
赵亮 +1
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0006] However, the research on the targets of eupterin A acting on breast cancer cells and its specific mechanism has not been reported yet.

Method used

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  • Application of ebracteolatain A in preparing drugs for treating breast cancer
  • Application of ebracteolatain A in preparing drugs for treating breast cancer
  • Application of ebracteolatain A in preparing drugs for treating breast cancer

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Embodiment Construction

[0030] The present invention will be further described below in conjunction with specific experimental steps.

[0031] 1) Experimental materials

[0032] Cell line: Human breast cancer cell MCF-7 was purchased from American Type Culture Collection (ATCC).

[0033] Reagents: Euphorbia euphorbia decoction pieces were purchased from Anhui, and the preparation and separation of euphorbia euphorbia was carried out by the methods in previous studies (Cao Qingqing, Li Shengjian, et al. Chemical constituents of euphorbia euphorbia and their breast cancer cytotoxic activity Research. Journal of Pharmaceutical Practice. 2019, 37(4)); PKD1 overexpression plasmid pcDNA3.1-PKD1 was constructed by Genescript, and the vector was pcDNA3.1; DMEM medium, penicillin / streptomycin solution, and fetal bovine serum were all purchased from Gibco; RNA extraction, reverse transcription, qRT-PCR and BCA protein quantification kits were purchased from Beijing Kangwei Century Company; transfection reagen...

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Abstract

The present invention belongs to the technical field of medicines, specifically relates to an application of ebracteolatain A (EA) in preparing drugs for treating breast cancer and reveals for the first time that the ebracteolatain A can inhibit expression of PKD1 protein in breast cancer cells in a dose-dependent manner. At the same time, the overexpression of PKD1 can significantly restore an inhibitory effect of the ebracteolatain A on proliferation of breast cancer cells. Finally, through mechanism research and analysis, the ebracteolatain A is confirmed to inhibit the proliferation of thebreast cancer cells by inhibiting MEK / ERK and PI3K / AKT signaling pathways mediated by the specific target PKD1. The research finds that the PKD1 plays a very important role in process of inhibiting the proliferation of breast cancer by the ebracteolatain A and is a key target in treatment process of the breast cancer.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of Ebracteolatain A (EA) in the preparation of drugs for treating breast cancer. Background technique [0002] Breast cancer is a common female malignancy. Worldwide, 1.7 million women are diagnosed with breast cancer each year, making it the second leading cause of cancer death in women. In 2018, according to statistics, about 2 million people around the world were diagnosed with new cases of breast cancer. According to statistics from the American Cancer Society, in 2016 in the United States, about 246,000 patients were diagnosed with invasive breast cancer, and 41,000 women died of breast cancer. In China, the morbidity and mortality of breast cancer are on the rise. Especially among women aged 30-59 living in urban areas, the incidence rate is twice that of rural areas. According to statistics, in 2014, 1.6 million people across the country we...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/12A61K45/00A61P35/00
CPCA61K31/12A61K45/00A61P35/00
Inventor 赵亮朱利君王莹李亚梅李盛建周艳卿周瑾
Owner 赵亮
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