Single-grain wheat amylose content detection method

Abstract

The invention belongs to the technical field of starch content detection, and discloses a single-grain wheat amylose content detection method which comprises the following steps: selecting a seed fromeach wheat variety, and weighing and crushing the seeds; adding HCl into each milligram of grain tissues, refrigerating and extracting overnight; adding NaOH into the residual extract to adjust the pH value of the extracting solution, sucking and uniformly mixing the mixture, and keeping the suspension at room temperature for 30 minutes; neutralizing with HCl, and diluting with 100 microliters ofdistilled water; extracting a part of the extracting solution to another centrifugal tube, diluting in the centrifugal tube according to a ratio of 1: 10, taking 25 [mu]l of the diluted product on anelisa plate, dropwise adding 100 [mu]l of 0.3% I: 1.5% KI stock solution into each hole, sucking for several times, and uniformly mixing; and standing for 10-15 minutes, reading the absorbance by using a microplate reader, and calculating the percentage of the amylose. According to the method, the amylose content in the single-grain wheat can be accurately determined, the amylose content can be rapidly and simply determined, and the detection cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of starch content detection, and in particular relates to a method for detecting amylose content of einkorn wheat. Background technique [0002] At present, the closest prior art: starch is one of the important components in wheat grains, and can be divided into amylose and amylopectin according to its molecular structure. Regular grains contain about 20% amylose, while waxy grains contain even less. The chemical properties of amylose molecules containing less branched have better nutritional value. Recent clinical studies have shown that amylose in food is important for lowering blood sugar and insulin. Since amylose is digested more slowly, it is beneficial to human health and can also increase the body's fat burning ability, which can help maintain a healthy weight, which may promote the demand for high-amylose cereals in the market in the future. Because the different proportions of amylose and amylop...

AI Technical Summary

Problems solved by technology

[0005](1) The existing detection method has the problem of Megazyme kit method, which is high cost

[0006](2) Existing detection methods are restricted by human factors, require very skilled detection personnel, and the operation steps are relatively complicated

[0007]Difficulty in solving the above technical problems: There are certain restrictions on cost control, so the number of samples that can be detected by these methods is limited, and the operation steps are complicated, and the detection personnel need to carry out Accurate results can only be measured after professional training

Images