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Method for measuring content of index components in urine and separating and identifying 20 metabolites after oral administration of emblic leafflower fruit tannin part

A technology of emblica and urine, which is applied in the field of chemical substance detection after oral administration of emblical tannins, can solve the problems of no simultaneous separation and identification of emblical tannin metabolites, and a small number of metabolites

Inactive Publication Date: 2020-06-26
BEIJING UNIV OF CHINESE MEDICINE
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Problems solved by technology

The number of GA and EA metabolites isolated and identified by existing methods is relatively small, and there is no method that can simultaneously isolate and identify emblical tannin metabolites

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  • Method for measuring content of index components in urine and separating and identifying 20 metabolites after oral administration of emblic leafflower fruit tannin part
  • Method for measuring content of index components in urine and separating and identifying 20 metabolites after oral administration of emblic leafflower fruit tannin part
  • Method for measuring content of index components in urine and separating and identifying 20 metabolites after oral administration of emblic leafflower fruit tannin part

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Embodiment Construction

[0054] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the embodiments and accompanying drawings. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0055] 1 Instruments and reagents

[0056] Instruments: Waters 1525 high-performance liquid chromatography (binary pump, Waters 2996 UV detector, Waters, USA); Thermo high-performance liquid chromatography-mass spectrometry (LTQ-Orbitrap XL HPLC-MS, ThermoFisher Company); 100,000 points One electronic balance (model: Sartorious BT 25S, Beijing Sartorius Instrument Co., Ltd.); high-speed centrifuge (Shanghai Anting Scientific Instrument Factory); vortex mixer (Shanghai Huxi Analytical Instrument Factory); 100-1000 pipette gun (Eppendorf); ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.,...

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Abstract

The invention relates to a method for detecting chemical substances after oral administration of an emblic leafflower fruit tannin part, in particular to a method for determining the content of indexcomponents in urine after oral administration of the emblic leafflower fruit tannin part and separating and identifying 13 prototype substances and 20 metabolites. The method has the advantages of simplicity and convenience in operation, good separation effect, good peak shape, high sensitivity, high precision, good reproducibility, good stability, high metabolite identification quantity and the like, and is a feasible method capable of effectively evaluating urine pathway metabolism and excretion conditions of the emblic leafflower fruit tannin part.

Description

technical field [0001] The invention relates to a method for detecting chemical substances after oral administration of tannin parts of emblica, in particular to a method for determining the content of index components in urine after oral administration of tannin parts of emblica and for separating and identifying 13 prototype substances and 20 metabolites. Background technique [0002] Drugs generally go through a series of processes such as absorption, distribution, metabolism, and excretion in the body. The drug efficacy, duration of drug efficacy, and toxic and side effects are closely related to the metabolism and excretion process of drugs in the body. The study of the metabolism and excretion of drugs is of great significance for the design of rational drug administration routes, dosage and clinical application, and can also provide a theoretical basis for the study of the substance basis of its efficacy. Urine is one of the main routes of drug excretion, and most met...

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/72
Inventor 张兰珍吴玲芳梁文仪常子豪张秋楠
Owner BEIJING UNIV OF CHINESE MEDICINE
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