Detection Panel and kit for pan-cancerous detection or targeted medication based on second generation sequencing and application

Abstract

The invention discloses a detection Panel and kit for pan-cancerous detection or targeted medication based on second generation sequencing and application of the detection Panel and kit. The detectionPanel comprises a target gene region used for detecting pan-cancerous mutation burden and mutation sites related to targeted medication, and an exon region related to pan-cancerous mutation sites. According to the invention, a liquid (blood) biopsy method is adopted, and a combined determination method is used for carrying out genetic change and protein biomarkers; the detection Panel not only can identify the existence of relatively early cancers, but also can locate origin organs of the cancers, has higher sensitivity, can better carry out combined detection, and provides more accurate support for treatment diagnosis of targeting drugs, chemotherapeutic drugs or immune drugs; and by designing and integrating common areas of different mutation-type information, the uniformity of the Panel is improved, the overall data volume of the Panel is reduced, and the cost is reduced.

Description

technical field [0001] The present invention relates to the technical field of biomedicine, in particular to a next-generation sequencing-based detection panel for pan-cancer detection or targeted drug use, a detection kit and its application. Background technique [0002] Currently, there are many cancer-related detection technologies, such as high-throughput sequencing, target region capture sequencing, liquid biopsy, tumor mutational burden, and MSI detection. [0003] Among them, High-Throughput Sequencing (High-Throughput Sequencing), also known as NextGeneration Sequencing (NGS), is relative to traditional Sanger Sequencing (Sanger Sequencing). [0004] On the basis of the sequencing method of Sanger et al., through technological innovation, the fragmented genomic DNA is connected to both sides of the adapter, and then different methods are used to generate millions of space-fixed PCR clone arrays. Each clone consists of multiple copies of a single library fragment. ...

AI Technical Summary

Problems solved by technology

Due to traditional sampling, the sampling site may not be able to obtain tumor tissue, and the blood may contain information of different mutated tumor cells