Extraction method of crocodile polypeptide

An extraction method and technology of crocodile polypeptide, applied in the field of extraction of crocodile polypeptide, can solve the problems of acid intolerance, protein allergy, poor absorption, etc., and achieve the effect of remarkable activity, strong versatility and high activity.

Pending Publication Date: 2020-08-14
广东焦点农业科技开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Protein is divided into animal protein, plant protein, single-cell protein and algae protein according to the source. These macromolecular proteins can supplement the protein needed by the human body to a certain extent, but they have disadvantages such as indigestibility, poor absorption, and acid resistance. Allergenic

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  • Extraction method of crocodile polypeptide

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Effect test

Embodiment 1

[0021] Example 1: Extraction of crocodile polypeptide.

[0022] A method for extracting crocodile polypeptides, comprising the following steps:

[0023] Step S10, soak 10 g of crocodile tissue in clear water at 70-80°C for 30 minutes, then wash it thoroughly with water, then place the washed crocodile tissue in a bottomed flask filled with 50 mL of distilled water, and place in an oil bath at 120°C Heating and stirring in medium temperature for 8h, fully hydrolyzed;

[0024] Step S20, taking the hydrolyzate obtained in step S10 for static precipitation, and then filtering to obtain the filtrate, which is a crude extract;

[0025] Step S30, taking the crude extract obtained in step S20, adding diatomaceous earth and activated carbon, stirring, standing for precipitation again, and filtering the filtrate to obtain a slightly light yellow pure fine extract;

[0026] Step S40, taking the fine extract obtained in step S30, and concentrating it using a vacuum water pump to obtain ...

Embodiment 2

[0030] Example 2: Antioxidant activity test of crocodile polypeptide.

[0031] This embodiment adopts DPPH method to measure the antioxidant activity of crocodile polypeptide, comprises the following steps:

[0032] Step S1 sample configuration: Accurately weigh a certain amount of sample, and use DMSO to prepare a mother solution with a concentration of 10 mg / mL for later use. During the experiment, dilute with absolute ethanol to the required concentration.

[0033] Step S2 DPPH configuration: DPPH needs to be prepared and used immediately. During the experiment, a certain amount of DPPH is accurately weighed and prepared into a solution with a concentration of 0.2 mmol / L with absolute ethanol.

[0034] Step S3 Antioxidant activity test: Add 100 μL of 2 mg / mL sample and 100 μL of 0.2 mmol / L DPPH solution to a 96-well microplate plate, mix well, react at room temperature for 30 minutes, and measure its absorbance at 517 nm in a full-wavelength microplate reader .

[0035] I...

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Abstract

The invention provides an extraction method of crocodile polypeptide, and belongs to the technical field of extraction of biological samples. The extraction method comprises the steps of performing heating for softening on crocodile tissue, then performing high-temperature constant-temperature hydrolysis, and then concentrating and drying hydrolysates to obtain crocodile polypeptide extract powder. The extraction method has the advantages of being simple to operate, high in extraction efficiency and high in extract activity.

Description

technical field [0001] The invention belongs to the technical field of biological sample extraction, in particular to a method for extracting crocodile polypeptides. Background technique [0002] As an essential nutrient for the human body, protein is extremely important to the life and health of the human body. Epidemiological survey results show that if the daily protein intake is too low, it is easy to suffer from esophageal cancer, gastric cancer, liver cancer and so on. Experts from the World Health Organization and the Food and Agriculture Organization of the United Nations believe that the daily protein intake of an ordinary citizen should not be less than 75g in a standard diet for normal activities. Protein is divided into animal protein, plant protein, single-cell protein and algae protein according to the source. These macromolecular proteins can supplement the protein needed by the human body to a certain extent, but they have disadvantages such as indigestibili...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/02A23J1/10
CPCA23J1/02A23J1/10
Inventor 张树华
Owner 广东焦点农业科技开发有限公司
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