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A kind of decellularized dermal matrix tissue engineering scaffold and preparation method thereof

A technology of decellularized dermis and matrix tissue, applied in tissue regeneration, medical science, prosthesis, etc., can solve the problems of long enzyme treatment time, cumbersome process, and difficulty in satisfying rapid cell growth, and achieves a simple and easy preparation method, Effect of promoting tissue repair and excellent biocompatibility

Active Publication Date: 2021-12-17
上海亚朋生物技术有限公司
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese patent CN109675112A discloses a method for preparing a human-derived acellular dermal matrix. The acellular allogeneic dermal matrix prepared by this method is a sheet-like structure with relatively dense gaps, which is difficult to meet the requirements of rapid cell growth and as a three-dimensional structure tissue engineering scaffold. and complex shape filling requirements
In addition, in order to realize the three-dimensionalization of the dermal matrix and obtain high porosity, Chinese patent CN109821071A discloses a hydrogel based on decellularized dermal matrix and its preparation method, but this method requires the introduction of protease digestion treatment such as pepsin and pancreatin , enzyme treatment will digest the protein in the dermal matrix, reduce the content of proteoglycan and other components in the dermal matrix, and the enzyme treatment time is longer, and the process is more cumbersome

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  • A kind of decellularized dermal matrix tissue engineering scaffold and preparation method thereof
  • A kind of decellularized dermal matrix tissue engineering scaffold and preparation method thereof
  • A kind of decellularized dermal matrix tissue engineering scaffold and preparation method thereof

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preparation example Construction

[0028] The invention provides a preparation method of an acellular dermal matrix tissue engineering scaffold, comprising the following steps:

[0029] (1) Immersing heterogeneous or allogeneic skin in saline solution to remove epidermal tissue to obtain dermal tissue;

[0030] (2) sequentially inactivating and decellularizing the dermal tissue to obtain a dermal matrix;

[0031] (3) drying and thinning the obtained dermis matrix sequentially to obtain dermis matrix fibers;

[0032] (4) The dermal matrix fibers are mixed with the dispersion liquid, followed by freeze-drying and cross-linking treatment in sequence to obtain the decellularized dermal matrix tissue engineering scaffold.

[0033] In the present invention, in the preparation method of the acellular dermal matrix tissue engineering scaffold, it is preferable to first scrape off the heterogeneous or allogeneic skin and hair with a blade, and then use a drum skin harvester to take the skin, and the thickness is prefer...

Embodiment 1

[0058] (1) Take the skin: scrape off the hair of the pigskin with a blade, take the skin with a drum skin machine, and cut the thickness of the dermal matrix (including the epidermis) to 1 mm.

[0059] (2) Peel off the skin: cut the heterogeneous or allogeneic skin into a size of 1cm×3cm, soak it in 1.5mol / L sodium chloride solution according to the ratio of 1g:5ml, shake it on the shaker for 12 hours, and gently peel off the skin with tweezers , to obtain dermal tissue.

[0060] (3) Virus inactivation: first wash the dermis tissue with purified water for 30 minutes, then soak the dermis tissue with 75% alcohol according to the ratio of 1g:5ml for 2 hours, and rinse with deionized water for 30 minutes.

[0061] (4) Decellularization: soak the dermal tissue in 1mol / L sodium hydroxide solution, the ratio of solid to liquid is 1g:5mL, and the soaking time is 12h; then soak the dermal tissue in 0.1% polyethylene glycol octylphenyl In an aqueous ether solution, the ratio of solid ...

Embodiment 2

[0065] (1) Take the skin: scrape off the hair of the pigskin with a blade, take the skin with a drum skin machine, and cut the thickness of the dermal matrix (including the epidermis) to 1 mm.

[0066] (2) Peeling off the skin: Cut the heterogeneous or allogeneic skin into a size of 1cm×3cm, immerse it in 1mol / L sodium chloride solution according to the ratio of 1g:4ml, shake it on the shaker for 12 hours, and gently peel off the skin with tweezers. Get dermal tissue.

[0067] (3) Virus inactivation: first wash the dermis tissue with purified water for 30 minutes, then soak the dermis tissue with 70% alcohol according to the ratio of 1g:4ml for 2 hours, and rinse with deionized water for 30 minutes.

[0068] (4) Decellularization: soak the dermal tissue in 0.5mol / L sodium hydroxide solution, the ratio of solid to liquid is 1g:4mL, and the soaking time is 6h; then soak the dermal tissue in 0.05% polyethylene glycol octylbenzene In the base ether aqueous solution, the ratio of ...

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Abstract

The invention relates to the technical field of tissue engineering scaffolds, in particular to an acellular dermal matrix tissue engineering scaffold and a preparation method thereof. In the present invention, heterogeneous or allogeneic skin is immersed in saline solution to remove epidermal tissue to obtain dermal tissue; the dermal tissue is sequentially subjected to inactivation treatment and decellularization treatment to obtain dermal matrix; the obtained dermal matrix is ​​sequentially dried and thinned to obtain Dermis matrix fibers; the dermis matrix fibers are mixed with the dispersion liquid and then subjected to freeze-drying and cross-linking treatment in sequence to obtain the decellularized dermis matrix tissue engineering scaffold. The acellular dermal matrix tissue engineering scaffold provided by the present invention does not require enzyme treatment and addition of auxiliary materials during preparation, and maintains the excellent biocompatibility of the acellular dermal matrix; and has high porosity and strong shape plasticity, which is conducive to the growth of cells Intake and penetration of nutrients to meet the repair of complex shape defects.

Description

technical field [0001] The invention relates to the technical field of tissue engineering scaffolds, in particular to an acellular dermal matrix tissue engineering scaffold and a preparation method thereof. Background technique [0002] Acellular dermal matrix is ​​an extracellular matrix obtained after decellularization of heterogeneous or allogeneic skin. Because of its good biocompatibility, it is widely used in burns, plastic surgery, tissue engineering scaffolds and other fields. Chinese patent CN109675112A discloses a method for preparing a human-derived acellular dermal matrix. The acellular allogeneic dermal matrix prepared by this method is a sheet-like structure with relatively dense gaps, which is difficult to meet the requirements of rapid cell growth and as a three-dimensional structure tissue engineering scaffold. And complex shape filling requirements. Therefore, constructing a sheet-like acellular dermal matrix into a three-dimensional structure with high po...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/50A61L27/56
CPCA61L27/362A61L27/3687A61L27/3691A61L27/50A61L27/56A61L2430/40A61L2300/412
Inventor 陈维明白玉龙陈金发周小垚胡凯姚杰侯立存李淼骆井万汪晶晶
Owner 上海亚朋生物技术有限公司
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