Enteromorpha polysaccharide with immunoregulatory activity and preparation method thereof

An immunoregulatory activity, prolifera polysaccharide technology, applied in the field of biomedicine, can solve problems such as glycosidic bond type and anti-tumor activity research, and achieve the effect of improving phagocytic ability and good safety.

Active Publication Date: 2022-06-28
SOUTHEAST UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Chinese patent CN108752495A reports a method for preparing enteromorpha alkali-soluble polysaccharides by enzymolysis and lye extraction, but this method does not check the monosaccharide composition, glycosidic bond type and antitumor activity of the prepared enteromorpha polysaccharides. Research
At present, there is no research report on the immunomodulatory activity of Enteromorpha polysaccharides. This invention is the first to study a low-molecular-weight polysaccharide extracted by alkaline method with immunomodulatory activity, which can provide a certain theoretical basis for the development and application of Enteromorpha polysaccharides.

Method used

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  • Enteromorpha polysaccharide with immunoregulatory activity and preparation method thereof
  • Enteromorpha polysaccharide with immunoregulatory activity and preparation method thereof
  • Enteromorpha polysaccharide with immunoregulatory activity and preparation method thereof

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Embodiment 1

[0019] 1) Separation and purification of low-molecular-weight polysaccharides of E. prolifera:

[0020] Pulverize the dried prolifera, put it into the extraction device, and extract the raw material powder three times with 95% ethanol as the extracting solution to remove pigments and small molecular impurities; Ionized water was used as the extraction solution (solid-liquid ratio = 1:10), extracted twice at 80 °C, and the water-extraction residue was collected. After drying, the water-extraction residue was extracted with 0.3M NaOH solution (solid-liquid ratio = 1:10), 90°C, extract twice, collect supernatant A, add 4 times the volume of ethanol to alcohol precipitation, let stand overnight to obtain flocculent precipitate B, freeze-dried, reconstitute the sample, add Sevage reagent (trichloromethane) : n-butanol = 4:1, v / v), add the reconstituted sample solution (sample: Sevage reagent = 4:1, v:v) and shake vigorously for 10 minutes to remove the protein, then stand for strat...

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Abstract

The invention relates to a preparation method of Enteromorpha polysaccharide PWEP‑1 and its immune regulation activity. The Enteromorpha polysaccharide of the present invention is extracted by water extraction and alcohol precipitation and separated and purified by ion exchange column and gel column chromatography. The distribution range of the Enteromorpha polysaccharide is 1000-5000Da, mainly composed of 87.42%, 7.10%, 1.67 %, 2.67% and 1.13% of mannose, rhamnose, galacturonic acid, galactose and xylose. In vitro experimental evaluation found that PWEP‑1 can improve the phagocytosis of RAW264.7 and promote the secretion of cytokines such as NO, TNF‑α and IL‑1β. PWEP‑1 can be added to functional foods as an immunomodulator to improve the theoretical basis for the development of new Enteromorpha products and improve the edible and commercial value of Enteromorpha.

Description

technical field [0001] The invention relates to the field of biomedicine, and in particular relates to a polysaccharide of Enteromorpha with immunoregulatory activity extracted by an alkaline method and a preparation method thereof. Background technique [0002] Enteromorpha Prolifera (Enteromorpha Prolifera) is a green algae, commonly known as moss strips and seaweed. It is widely distributed in the intertidal zone of the world, especially in China, the Baltic Sea and Chile, and is widely distributed in the eastern coast of my country. Chinese medical classics record that prolifera has been eaten and used medicinally by coastal residents in my country since ancient times. In recent years, due to the proliferation of prolifera, the ecological balance of the marine environment has been destroyed, and even serious "green tide" hazards have been caused. Therefore, how to make better use of this marine resource and turn waste into treasure has become a hot area of ​​research an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A23L33/125
CPCC08B37/0003C08B37/006A23L33/125A23V2002/00A23V2200/324A23V2250/202A23V2250/51A23V2300/14
Inventor 廖志新赵士峰侯培蕾冯艳孙波李妍刘玲菲
Owner SOUTHEAST UNIV
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