36results about How to "Has immunomodulatory activity" patented technology

The invention relates to a moisturizing composition, and a preparation method and application thereof, which belong to the field of cosmetics. The moisturizing composition is prepared from the following components in parts by weight: 1.0 to 5.0 parts of sodium hyaluronate, 2.0 to 10.0 parts of carboxymethyl deacetyl chitosan, 30.0 to 60.0 parts of dendrobium officinale extract, and 25.0 to 67.0 parts of deionized water. The moisturizing composition can well form a netted natural biomimetic membrane with skin, the sodium hyaluronate performs the water supplementation and moisturizing in the biomimetic membrane, the carboxymethyl deacetyl chitosan forms a biomimetic sterile membrane, so that natural moisturizing factors such as the sodium hyaluronate can be effectively locked, the water loss rate is decreased; and the dendrobium officinale polysaccharide is combined with the natural biomimetic sterile membrane, so that the moisturizing lasting property after long-term use can be further improved, the restoration effect is improved, and the dryness and allergy problems of the skin can be fundamentally solved. The moisturizing composition has multidimensional efficacy such as moisturizing function, restoration function and skin barrier increasing function.

The invention discloses a preparing method of hygienic liquid of pine mushroom, which comprises the following steps: fermenting pine mushroom deeply; filtering the fermented liquid from mycelium; centrifuging ferment liquid and grinded mycelium through ultrasound; blending fermented original liquid or composite condensed liquid and honey according to weight rate at 100:2-5; allocating; sterilizing; canning; obtaining the product with multiple biological active material and amino acids; possessing immune adjusting activity in the animal experiment.

The invention discloses a method for producing giant salamander polypeptide powder liver protection capsules. The method comprises the following steps of: performing low-temperature drying, grinding, fat removal, controllable composite enzyme digestion, centrifugation, filtration, two-time membrane filtration, vacuum concentration, freeze drying and crushing on fresh giant salamander meat to obtain giant salamander polypeptide powder; encapsulating the giant salamander polypeptide powder, and packaging to obtain a finished product.

The invention discloses compound indigowoad root oral liquid for improving poultry immunity and a preparation method thereof and aims at providing compound indigowoad root oral liquid for improving organism immunity and increases vaccine immunity effect and a preparation method thereof. Each 100 liters of the oral liquid comprises the following components: 0.5 to 20kg of indigowoad root amylose, 0.5 to 20kg of astragalus amylose raw material medicament containing 50 percent of astragalus amylose, 0.5 to 10kg of epimedium amylose raw material medicament containing 50 percent of epimedium amylose, 0.5 to 10kg of morinda extract, 50g of potassium sorbate and the balance of injection water. In the method, an amylose component with immunologic enhancement effect is selected from an indigowoad root medicinal material and mixed with restorative Chinese medicaments with the same immunologic enhancement effect, such as astragalus, epimedium and morinda, to produce a synergetic effect to enhance treatment effect. The oral liquid has good treatment effect on low immunity of poultry caused by disease or breeding conditions and can strengthen immune effect of a vaccine.

The invention discloses an application of rainbow trout IFN-gamma2 (Interferon-gamma2) in preparing an anti-IHNV (Infectious Haematopoietic Necrosis Virus) product. The invention provides an application of rtIFN-gamma2 protein, an encoding gene or a recombinant carrier with the encoding gene in preparing a product for inhibiting infectious hemopoietic organ necrosis viruses. The amino acid sequence of the rtIFN-gamma2 protein is a sequence 2 as shown in a sequence table. Experiment shows that IFN-gamma2 has the anti-IHNV activity, the situation that no effective anti-IHNV preparation is available in China at present is changed, meanwhile the interferon-gamma also has the immunity adjustment activity, and the rtIFN-gamma2 can be further developed into a natural immunity enhancing agent or an adjuvant for rainbow trout.

The invention discloses an agaricus bisporus stem yoghourt processing technology. The agaricus bisporus stem yoghourt processing technology comprises the following processes of: generating milk by using raw materials, inspecting, standardizing, filtering, mixing, blending, homogenizing, filtering, sterilizing, cooling, inoculating, filling, fermenting, post-maturating and finishing to obtain finished agaricus bisporus stem yoghourt. The agaricus bisporus stem yoghourt which is prepared from agaricus bisporus stem slurry with the agaricus bisporus polysaccharide content of 1.563mg / g by using the technology is rich in edible granulose, multi-amino acids and mineral substances, is fine in mouthfeel, has an aromatic flavor, and is high in nutrition value, and the main technical index of the agaricus bisporus stem yoghourt corresponds to the relevant provisions of GB19302-2010.

The invention discloses an application of an antibacterial peptide C-BF in preparation of medicines for preventing and treating weanling stress of piglets. The intestinal morphology can be improved by the antibacterial peptide C-BF; the villus height can be increased; the intestinal inflammation is relieved; the growth performance of weaned pigs is improved; the diarrhea rate is lowered; and the antibacterial peptide C-BF is dosed through intraperitoneal injection.

The invention discloses a method for separating a neutral longan polysaccharide fraction. The method comprises the following steps of: (1) selecting longan fruit pulp water extract, and removing most of protein and pigment from the longan fruit pulp water extract through D301-F anion exchange resin to obtain crude longan polysaccharide; (2) separating and purifying the crude longan polysaccharide by a diethylaminoethyl DEAE-52 cellulose ion exchange chromatographic column, eluting with deionized water, collecting longan polysaccharide eluent, concentrating and freeze-drying under vacuum to obtain neutral longan polysaccharide powder; and (3) allowing the neutral longan polysaccharide to pass through a SephadexG-100 gel permeation chromatographic column, eluting with the deionized water, collecting different kinds of longan polysaccharide fraction eluent, concentrating and freeze-drying under vacuum to obtain the neutral longan polysaccharide fraction. By the method, a neutral single polysaccharide component can be separated and prepared.

The invention discloses a method for producing giant salamander polypeptide powder, which comprises the following steps of low-temperature drying, crushing, fat removing, controlled enzymatic hydrolysis with a compound enzyme, centrifugation, coarse filtration, two times of membrane filtration, vacuum concentration, freeze drying, and pulverization of fresh giant salamander meat to obtain the giant salamander polypeptide powder.

The invention discloses natural antioxidant peptide Macrotympanain-Al and a preparation method and application thereof. The natural antioxidant peptide Macrotympanain-Al is a gene-encoded product, and a sequence from an N end to a C end is RLFKCYKPDS. The natural antioxidant peptide Macrotympanain-Al is prepared from skins of Odorrana graminea Boulenger which are typical in China, and in frog skins, the natural antioxidant peptide Macrotympanain-Al is mainly used for protecting exposed skins from being injured by ultraviolet rays; and the antioxidant polypeptide Macrotympanain-Al is applied to preparation of cosmetics. The natural antioxidant peptide Macrotympanain-Al returns to the origin, is purely natural, and accords with consumption requirements of common people at present. The activity of the natural antioxidant peptide Macrotympanain-Al is 1000 times stronger than that of the conventional antioxidant BHT (butylated hydroxytoluene). The natural antioxidant peptide Macrotympanain-Al not only has antioxidant activity, but also has a certain antibiotic activity, immunoregulation activity and the activity for inhibiting the formation of melanin.

The invention discloses a ganoderma lucidum spore powder wall polysaccharide, the ganoderma lucidum spore powder wall polysaccharide is glucan whose main chain is formed by four glycoside residues andbranch chains are formed by beta-D-Glcp-(1-> and beta-D-Glcp-(1->4)-beta-D-Glcp-(1->. The invention further discloses a preparation method of the ganoderma lucidum spore powder wall polysaccharide. In the ganoderma spores processed with the method, two kinds of beta-glucans with multiple branches can be obtained, the cellular level doesn't show immunization, unexpectedly, in various animal immunization model experiments, the polysaccharide has various immunoregulatory activities and is the main active ingredient in the ganoderma lucidum spore powder. Therefore, the ganoderma lucidum spore powder wall recycle application range is expanded greatly.

The invention relates to an application of lycium ruthenicum polysaccharides in preparation of immunoregulation medicines or health-care products. The lycium ruthenicum polysaccharides refer to a group of glycoprotein complex which have the molecular weight of 80-22kDa and are obtained by performing hot water extraction, ethanol precipitation, deproteinization and discoloring on lycium ruthenicum fruits. The animal experiments prove that the lycium ruthenicum polysaccharides have the effects of obviously improving the spleen and thymus indexes of immunosuppressed mice and improving the lymphocytes level of the immunosuppressed mice; the delayed type hypersensitivity of the immunosuppressed mice is enhanced; the antibody secreting level of the immunosuppressed mice is improved. The cell experiments prove that the spleen cell proliferation rate of the mice can be obviously improved through the lycium ruthenicum polysaccharides, macrophage cell lines RAW264.7 of the mice are promoted to secrete TNF (tumor necrosis factor)-alpha, NO and other immune factors, and the lycium ruthenicum polysaccharides are proved to have high immunological competences.

The invention relates to bean residue dietary fiber active peptide face-beautifying low-alcohol-content rice wine and a preparation method thereof. The rice wine is characterized by being prepared with, by weight, 200-300 parts of fresh bean residue, 30-35 parts of rice bran protein, 100-250 parts of rice wine, 5-7 parts of kudzu vine root extract, 3-5 parts of licorice extract, 5-7 parts of rose apple seed extract and 10-13 parts of perilla oil. Through the steps of drying, smashing, pickling, fermenting and enzymolyzing bean residue, beany flavor of the rice wine is reduced, and the rice wine is better in taste and more exquisite in quality and is fully utilized. A new drinking concept of seeking stimulus while pursing nutrition and health during drinking of people is met, a new nutrition absorbing concept of direct absorption is provided for people, improving of immunity is facilitated, and the rice wine has functions of resisting oxidation, lowering blood pressure and blood fat and protecting liver and is incomparable for any non-peptide wine.

The invention relates to a natural antioxidant peptide Tiannanin-beta1 and preparation method and application thereof. The natural antioxidant peptide Tiannanin-beta1 is a gene-encoding product and has the following sequence from the N-terminal to C-terminal: VVKFPYRCKAAF. The natural antioxidant peptide is prepared from the skin of Odorrana frogs which can only be found in China and exits in the frog skin to play a major role of protecting the exposed skin from ultraviolet injury. The antioxidant peptide Tiannanin-beta1 is applied to cosmetic production, is original and pure natural, and conforms to the consumption requirements for modern people. The activity of the antioxidant peptide is 1000 times higher than that of the conventional antioxidant BHT. Besides the antioxidant activity, the peptide Tiannanin-beta1 also has antimicrobial activity, immunoregulatory activity and melanin formation inhibitory activity.

The invention discloses an extracellular polysaccharide complex of Chlorella protothecoides with immunity regulation activity as well as a preparation method and application thereof. The extracellularpolysaccharide complex of the Chlorella protothecoides disclosed by the invention is extracted from Chlorella protothecoides CS-41 fermentation broth, and is specifically prepared by the following steps: carrying out filtration, centrifuging, concentration and ethanol precipitation, and removing proteins by adopting a Sevage method; and then, carrying out separation and purification procedures, including anion exchange chromatography, gel exclusion chromatography and the like, so as to obtain the extracellular polysaccharide complex of the Chlorella protothecoides. The prepared extracellularpolysaccharide complex of the Chlorella protothecoides is white powder of which the average relative molecular weight is 170 kDa; and the extracellular polysaccharide complex of the Chlorella protothecoides is composed of lipid and polysaccharides, wherein the lipid contains palmitic acid and stearic acid, and the polysaccharides are composed of rhamnose, xylose, mannose and glucose. The extracellular polysaccharide complex of the Chlorella protothecoides disclosed by the invention is clear in compositions, and has the effect of immunity regulation; and moreover, the extracellular polysaccharide complex is capable of significantly reducing levels of inflammatory factors (ROS, iNOS, IL-6 and TNF-alpha). Thus, the extracellular polysaccharide complex of the Chlorella protothecoides has broadprospects of application in clinical as well as related medical and health-caring fields.

The invention discloses a preparation method of a dandelion root polysaccharide sulfation modified product and application of the dandelion root polysaccharide sulfation modified product. The polysaccharide content of the polysaccharide sulfation modified product is increased by 60% compared with the content before modification. The method comprises the following steps of: selecting an optimal extraction process through a plurality of groups of tests, obtaining crude polysaccharide by adopting a water extraction and alcohol precipitation method, deproteinizing by adopting a Sevag method, separating by adopting DEAE Sepharose Fast Flow, purifying by adopting sephadex to obtain dandelion root polysaccharide with high purity, and modifying by adopting a chlorosulfonic acid-dimethylformamide method to obtain a dandelion root polysaccharide sulfation modified product. According to the method, waste dandelion root resources are fully utilized, waste is turned into wealth, the dandelion root polysaccharide with immunoregulatory activity and the sulfated modified product of the dandelion root polysaccharide are obtained, and sustainable application of dandelion medicinal resources is achieved.

The invention discloses a natural antioxidant peptide (Rotodorin-D1) and a preparation method and application thereof. The natural antioxidant peptide (Rotodorin-D1) is a gene coded product, a sequence from an N end to a C end is KSSDVKYYLAKCTTDVQ. The natural antioxidant peptide (Rotodorin-D1) is made of skin of Odorrana graminea Boulenger which only exists in China; in Batrachia skin, Odorrana graminea Boulenger has a main role in protecting skin exposed to the outside from being damaged by ultraviolet rays, and the polypeptide (Rotodorin-D1) with antioxidative activity is used in preparation of cosmetics. Origin returning and all natural effects meet consuming requirements of current publics. Compared with the conventional antioxidant (BHT), the Rotodorin-D1 has activity which is improved by 1000 times; besides the antioxidant activity, the Rotodorin-D1 has certain antibacterial activity, immunoregulation activity, and melanin forming restraining activity.

The invention relates to an antiaging bean milk fiber active peptide rice wine and a preparation method thereof. The invention is characterized in that the rice wine is prepared from the following raw materials in parts by weight: 300-350 parts of fresh bean dreg, 30-35 parts of rice bran protein, 100-150 parts of rice wine, 5-7 parts of Coptis chinensis pollen, 5-7 parts of royal jelly dry powder, 5-7 parts of grape seed extract and 5-7 parts of deep sea fish oil. The effective components of the antiaging bean milk fiber active peptide rice wine have the health-care functions of resisting oxidation, preventing aging, beautifying the face, promoting blood circulation, enhancing the immunity of human body and the like.

The invention discloses an immunoactive peptide BP1 in fermented milk. The amino acid sequence of the active peptide BP1 is NQFLPYPYYAKPA. The active peptide BP1 can obviously promote secretion of cell factors TNF-alpha and IL-6, and has a good immunological enhancement effect. The invention further discloses an enrichment method of the active peptide. Milk fermentation parameters are optimized through a response surface method by taking the content of the active peptide BP1 as an index, so that the content of the finally obtained active peptide BP1 is 0.213 mg / mL, which is obviously higher than that of a conventional fermentation technology. The method can be used for increasing the content of the immunoactive peptide in the fermented milk; a process instruction is provided for further exploration of extraction of a biological active peptide in the fermented milk; the immunoactive peptide BP1 in the fermented milk has important significance for developing dairy products and health products which have immune modulating functions.

The invention discloses a method for producing giant salamander polypeptide powder liver protection capsules. The method comprises the following steps of: performing low-temperature drying, grinding, fat removal, controllable composite enzyme digestion, centrifugation, filtration, two-time membrane filtration, vacuum concentration, freeze drying and crushing on fresh giant salamander meat to obtain giant salamander polypeptide powder; encapsulating the giant salamander polypeptide powder, and packaging to obtain a finished product.

The invention discloses an immunoactive peptide BP in fermented milk 1 , the active peptide BP 1 The amino acid sequence of NQFLPYPYYAKPA is NQFLPYPYYAKPA, and the active peptide BP1 can significantly promote the secretion of cytokines TNF-α and IL-6, and has a good immune-promoting effect. The invention also discloses a method for enriching the active polypeptide. The invention uses the active peptide BP 1 content as an index, the parameters of milk fermentation were optimized by response surface methodology, and finally the active peptide BP was obtained 1 The content reaches 0.213 mg / mL, which is significantly better than the conventional fermentation technology. This method can be used to increase the content of immunoactive peptides in fermented milk, provide process guidance for further exploration of extracting bioactive peptides from fermented milk, and provide guidance for the development of dairy products with immune regulation function and health care products are of great significance.

The invention discloses a method for separating a neutral longan polysaccharide fraction. The method comprises the following steps of: (1) selecting longan fruit pulp water extract, and removing most of protein and pigment from the longan fruit pulp water extract through D301-F anion exchange resin to obtain crude longan polysaccharide; (2) separating and purifying the crude longan polysaccharideby a diethylaminoethyl DEAE-52 cellulose ion exchange chromatographic column, eluting with deionized water, collecting longan polysaccharide eluent, concentrating and freeze-drying under vacuum to obtain neutral longan polysaccharide powder; and (3) allowing the neutral longan polysaccharide to pass through a SephadexG-100 gel permeation chromatographic column, eluting with the deionized water, collecting different kinds of longan polysaccharide fraction eluent, concentrating and freeze-drying under vacuum to obtain the neutral longan polysaccharide fraction. By the method, a neutral single polysaccharide component can be separated and prepared.

The invention relates to an echinacea extract and a preparation method thereof, and belongs to the technical field of medicine. The fresh echinacea is harvested from mid-August to the end of Septembereach year and cut into sections of 5-15 cm, hot water at 90-98 DEG C is firstly added to a continuous countercurrent extractor, and then the cut fresh echinacea is put for enzyme inactivation; the pHvalue is adjusted to 4.2-6.2, and spraying extraction is performed for 60-90min to obtain an extraction solution; then the extraction solution passes through D941 macroporous weak base anion exchangeresin (each resin column is loaded with 330-780 kg of resin), and a column liquid is collected; the column liquid is concentrated under reduced pressure to a relative density of 1.12-1.18, ethanol with the volume of 2.5-3.2 times is added to obtain an alcohol precipitate, and then concentration under reduced pressure is continued to recover the ethanol to obtain an extract; and the extract is subjected to spray drying to obtain the echinacea extract. By use of the extraction method of the present invention, technologies such as raw material harvesting, continuous extraction, pH adjustment andresin purification are completed in a relatively short time, and the cichoric acid content is increased to 4%-12%. Large-scale production reduces the cost of the echinacea extract and is beneficial to marketization.

The invention relates to a natural antioxidant polypeptide mixture and preparation method and application of the mixture. The natural antioxidant polypeptide mixture is prepared from the skin of Odorrana frogs which can only be found in China and exits in frog skin to protect the exposed skin from ultraviolet injury. The antioxidant polypeptide mixture is applied to the preparation of cosmetics. The antioxidant polypeptide mixture is original and pure natural and conforms to the consumption needs of modern people, and the activity of the antioxidant polypeptide mixture is 1000 times higher than that of the conventional antioxidant butylated hydroxytoluene (BHT). Besides the antioxidant activity, the polypeptide mixture also has antimicrobial, immunoregulatory and melanin formation inhibiting activities.

The invention discloses an application of rainbow trout IFN-gamma2 (Interferon-gamma2) in preparing an anti-IHNV (Infectious Haematopoietic Necrosis Virus) product. The invention provides an application of rtIFN-gamma2 protein, an encoding gene or a recombinant carrier with the encoding gene in preparing a product for inhibiting infectious hemopoietic organ necrosis viruses. The amino acid sequence of the rtIFN-gamma2 protein is a sequence 2 as shown in a sequence table. Experiment shows that IFN-gamma2 has the anti-IHNV activity, the situation that no effective anti-IHNV preparation is available in China at present is changed, meanwhile the interferon-gamma also has the immunity adjustment activity, and the rtIFN-gamma2 can be further developed into a natural immunity enhancing agent or an adjuvant for rainbow trout.

The invention discloses a preparing method of hygienic liquid of pine mushroom, which comprises the following steps: fermenting pine mushroom deeply; filtering the fermented liquid from mycelium; centrifuging ferment liquid and grinded mycelium through ultrasound; blending fermented original liquid or composite condensed liquid and honey according to weight rate at 100:2-5; allocating; sterilizing; canning; obtaining the product with multiple biological active material and amino acids; possessing immune adjusting activity in the animal experiment.

The invention relates to an active peptide preblend health-care rice wine capable of strengthening the spleen and stomach and a preparation method thereof. The invention is characterized in that the health-care rice wine is prepared from the following raw materials in parts by weight: 200-225 parts of fresh bean dreg, 30-35 parts of rice bran protein, 100-150 parts of rice wine, 5-7 parts of Chinese wolfberry extract, 5-7 parts of tuckahoe extract, 10-13 parts of Chinese yam powder and 3-5 parts of perilla. The bean dreg is dried, pulverized, pickled, fermented and subjected to enzymolysis and the like to reduce the beany flavor, and thus, has better mouthfeel and fine texture, thereby fully utilizing the bean dreg. The health-care rice wine satisfies the demands of people for excitement, nutrition and health, provides a new concept of directly absorbing nutrients for people, is beneficial to enhancing the immunologic function, has the functions of resisting oxidation, lowering the blood pressure and blood fat, protecting the liver and the like, and is incomparable with any other non-peptide wine.

The invention relates to a lung-moistening and beauty-maintaining bean dreg dietary fiber low-alcohol peptide wine and a preparation method thereof. The wine is characterized by being prepared from the following raw materials in parts by weight: 300-350 parts of fresh bean dregs, 30-35 parts of rice bran protein, 100-150 parts of rice wine, 15-20 parts of bitter gourd powder, 5-7 parts of tangerine peel micro powder, 30-35 parts of aloe juice, and 7-9 parts of black fungus polysaccharide. The active ingredients of the lung-moistening and beauty-maintaining bean dreg dietary fiber low-alcohol peptide wine provided by the invention have the health effects of clearing fire and moistening lung, improving the human immunity, disinfecting, diminishing inflammation, maintaining beauty and keeping young, and the like.