Preparation method of dandelion root polysaccharide sulfation modified product and application of dandelion root polysaccharide sulfation modified product
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A polysaccharide sulfuric acid, dandelion technology, applied in application, polysaccharide/gum-containing food ingredients, medical preparations containing active ingredients, etc. Effect
Pending Publication Date: 2021-11-09
INST OF AGRI MECHANIZATION XINJIANG AGRI INST
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[0004] At present, domestic and foreign research on dandelion root polysaccharides mainly fo
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Embodiment 1
[0025] The preparation method of the sulfated modification product of dandelion root polysaccharide comprises the following steps:
[0026] (1) Pulverize the dandelion root as a raw material, put it in an extraction tank, add 10 times its volume of water, extract with boiling water for 3 hours, and repeat twice. Filter the supernatant to remove impurities, concentrate properly, use Sevag reagent to centrifuge to remove protein, centrifuge, take the supernatant, add 2 times the volume of absolute ethanol, stir evenly, and precipitate overnight. Suction filtration was carried out with a Buchner funnel and filter paper to obtain a precipitate. Re-dissolve the precipitate with distilled water, evaporate ethanol in a water bath at 50°C, and freeze-dry with a freeze dryer to obtain crude polysaccharide from dandelion root;
[0027] (2) Separation of crude polysaccharides from dandelion roots
[0028] Weigh the dandelion root crude polysaccharide prepared in step (1), add distilled...
Embodiment 2
[0032] (1) Pulverize the dandelion root as a raw material, put it in an extraction tank, add water 10-30 times its volume, extract with boiling water for 5 hours, and repeat 3 times. Filter the supernatant to remove impurities, concentrate properly, use Sevag reagent to centrifuge to remove protein, centrifuge, take the supernatant, add 4 times the volume of absolute ethanol, stir evenly, and precipitate overnight. Suction filtration was carried out with a Buchner funnel and filter paper to obtain a precipitate. Re-dissolve the precipitate with distilled water, evaporate ethanol in a water bath at 60°C, and freeze-dry with a freeze dryer to obtain crude polysaccharide from dandelion root;
[0033] (2) Separation of crude polysaccharides from dandelion roots
[0034] The dandelion root crude polysaccharide prepared in step (1) was weighed, dissolved in distilled water, centrifuged at a speed of 12000 rpm, the supernatant was taken for loading, and eluted with distilled water a...
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Abstract
The invention discloses a preparation method of a dandelion root polysaccharide sulfation modified product and application of the dandelion root polysaccharide sulfation modified product. The polysaccharide content of the polysaccharide sulfation modified product is increased by 60% compared with the content before modification. The method comprises the following steps of: selecting an optimal extraction process through a plurality of groups of tests, obtaining crude polysaccharide by adopting a water extraction and alcohol precipitation method, deproteinizing by adopting a Sevag method, separating by adopting DEAE Sepharose Fast Flow, purifying by adopting sephadex to obtain dandelion root polysaccharide with high purity, and modifying by adopting a chlorosulfonic acid-dimethylformamide method to obtain a dandelion root polysaccharide sulfation modified product. According to the method, waste dandelion root resources are fully utilized, waste is turned into wealth, the dandelion root polysaccharide with immunoregulatory activity and the sulfated modified product of the dandelion root polysaccharide are obtained, and sustainable application of dandelion medicinal resources is achieved.
Description
technical field [0001] The invention relates to a plant polysaccharide, in particular to a preparation method of a dandelion root polysaccharide with immunoregulatory activity and a sulfated modified product thereof and an application of the sulfated modified product of the dandelion root polysaccharide. Background technique [0002] Dandelion is a perennial herb of Compositae, widely distributed in various regions of my country, and has a wide variety and large output. Its medicinal value has long been recorded in various medical classics. The earliest record about dandelion was found in "Tang Materia Medica": "It tastes sweet, flat, and non-toxic. Housewives have mastitis." In "Compendium of Materia Medica", it is recorded that dandelion is mainly used Women's mastitis, detoxification, dissipating stagnant qi, detoxifying heat, eliminating malignant swelling, tuberculosis, and edema. Dandelion roots, stems, and leaves all have high medicinal value, and the chemical compos...
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