Natural antioxidant peptide (Rotodorin-D1), and preparation method and application thereof

A rotodorin-d1, natural anti-oxidation technology, applied in the preparation method of peptides, chemical instruments and methods, cosmetic preparations, etc., can solve the problems of limited oxidation activity and high price

Inactive Publication Date: 2012-07-18
梁醒财
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Common antioxidants in cosmetics, such as vitamin C, vitamin E and SOD, etc., have limited oxidative activity
However, natural astaxanthin and other natural antioxidant products are generally too expensive due to their “natural” selling point, which is difficult to be accepted by the public.

Method used

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  • Natural antioxidant peptide (Rotodorin-D1), and preparation method and application thereof

Examples

Experimental program
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Embodiment Construction

[0020] ① Acquisition of skin secretions: Stimulate the skin of the Yunnan stinky frog with a voltage of 6V for 6s, then wash the body of the stinky frog with 25mM Tris-HCl buffer (pH=7.8), collect the skin secretions, and put the collected secretions in Centrifuge at 4000rpm for 15min, vacuum freeze-dry the supernatant, and store at -80°C for later use;

[0021] ②Purification of polypeptide: redissolve the lyophilized supernatant sample, put it on a Sephadex G-75 gel filtration column equilibrated with 25mM Tris-HCl buffer (pH=7.8, containing 0.1M NaCl) in advance, and use 0.3ml / min flow rate for elution, collected every 10min. The eluted product was monitored at 280nm, the separation and purification map was drawn, and the antioxidant active ingredients were collected, and used C 4 The column was freeze-dried after removing the salt inside. Afterwards, the sample was dissolved with 20 mM NaAc-HAc buffer (pH=4.0), purified with a Resource S separation column equilibrated wi...

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Abstract

The invention discloses a natural antioxidant peptide (Rotodorin-D1) and a preparation method and application thereof. The natural antioxidant peptide (Rotodorin-D1) is a gene coded product, a sequence from an N end to a C end is KSSDVKYYLAKCTTDVQ. The natural antioxidant peptide (Rotodorin-D1) is made of skin of Odorrana graminea Boulenger which only exists in China; in Batrachia skin, Odorrana graminea Boulenger has a main role in protecting skin exposed to the outside from being damaged by ultraviolet rays, and the polypeptide (Rotodorin-D1) with antioxidative activity is used in preparation of cosmetics. Origin returning and all natural effects meet consuming requirements of current publics. Compared with the conventional antioxidant (BHT), the Rotodorin-D1 has activity which is improved by 1000 times; besides the antioxidant activity, the Rotodorin-D1 has certain antibacterial activity, immunoregulation activity, and melanin forming restraining activity.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a polypeptide with antioxidant activity-natural antioxidant peptide Rotodorin-D1, its preparation method and its application in cosmetics. Background technique [0002] Free radicals are harmful compounds produced in the oxidation reaction of the body. They have strong oxidative properties and can damage the tissues and cells of the body, causing chronic diseases and aging effects. Under normal physiological conditions, the generation and removal of free radicals in the body are in a state of dynamic balance, but when the body is in an unfavorable environment and with age, the body will produce too many free radicals or remove them too slowly. Free radical scavengers, also known as antioxidants, can remove excess free radicals in the body and reduce their damage to the body. [0003] At present, it has been recognized that there are two sets of antioxidant systems in natural organis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08C07K1/16A61K8/64A61Q19/08
Inventor 梁醒财
Owner 梁醒财
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