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Ganoderma lucidum spore powder wall polysaccharide and extraction method thereof

A technology of Ganoderma lucidum spore powder and cell wall polysaccharide, which is applied in the field of biopharmaceuticals, and can solve the problems of Ganoderma lucidum spores that are not easily digested by the human body, composition changes, chemical composition damage, etc.

Inactive Publication Date: 2018-08-31
ZHEJIANG SHOUXIANGU PHARMA CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Ganoderma lucidum spore powder is the essence of Ganoderma lucidum, which is the main medicinal effect and health-care nutritional ingredient in Ganoderma lucidum, but the spores of Ganoderma lucidum are not easily digested by the human body after taking it
At present, the method of breaking the wall of Ganoderma lucidum spore powder is mainly to use chemical method to break the wall or to heat the wall. The components of the solvent are difficult to separate and clean. When people take it with other medicines, the residual chemical substances may react with other medicines, or the chemical components may directly cause damage to the human body; the method of heating, due to repeated heating, the temperature is too high, spores The active substance contained in it is killed or its composition is changed

Method used

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  • Ganoderma lucidum spore powder wall polysaccharide and extraction method thereof
  • Ganoderma lucidum spore powder wall polysaccharide and extraction method thereof
  • Ganoderma lucidum spore powder wall polysaccharide and extraction method thereof

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Effect test

Embodiment 1

[0027] Such as figure 1 As shown, weigh Ganoderma lucidum spore powder, extract twice with boiling water, dry the residue after centrifugation, break the wall, degrease, and then extract with boiling water. After centrifugation, the supernatant is classified into ethanol precipitation, and GLW and Cell wall polysaccharides of two Ganoderma lucidum spore powders of GLM.

[0028] Among them, the proportion of GLW polysaccharide is high, and the polysaccharide content is more than 70%. It is the main component of ganoderma lucidum cell wall polysaccharide, and this part of polysaccharide is further separated and purified. GLW was precipitated with 50% ethanol, and the precipitated part was named GLSW50. GLSW50 was separated and purified by ion-exchange column chromatography, gradient eluted with water and sodium chloride, and each collected tube was detected by the phenol-sulfuric acid method. The polysaccharide curve obtained was as follows: figure 2 , found a total of 2 poly...

Embodiment 2

[0038] Example 2 Biological activity of GLSW50A-I and GLSW50A-II

[0039] Since the polysaccharide of Ganoderma lucidum spore powder treated above has no obvious biological activity on the models of lymphocytes and macrophages, the main polysaccharide component GLSW50A obtained from the wall of Ganoderma lucidum spore powder shows better biological activity in animal experiments, significantly Improvement of carbon clearance index in cyclophosphamide-immunosuppressed mice. Therefore, the activity test verification is carried out by animal experiments.

[0040] Using the Ganoderma lucidum spore powder wall polysaccharide GLSW50A purified in Example 1 as a positive control, the dose is 0.2g / Kg; the spore powder wall shell sample (GLP1, polysaccharide content is 2%) of ultrafine grinding and breaking the wall for 0.5h, three groups of doses 0.5g / kg, 1g / kg, and 2g / kg respectively; the corresponding untreated spore powder wall shell GLP2 is selected in addition, and the dose is 2g...

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Abstract

The invention discloses a ganoderma lucidum spore powder wall polysaccharide, the ganoderma lucidum spore powder wall polysaccharide is glucan whose main chain is formed by four glycoside residues andbranch chains are formed by beta-D-Glcp-(1-> and beta-D-Glcp-(1->4)-beta-D-Glcp-(1->. The invention further discloses a preparation method of the ganoderma lucidum spore powder wall polysaccharide. In the ganoderma spores processed with the method, two kinds of beta-glucans with multiple branches can be obtained, the cellular level doesn't show immunization, unexpectedly, in various animal immunization model experiments, the polysaccharide has various immunoregulatory activities and is the main active ingredient in the ganoderma lucidum spore powder. Therefore, the ganoderma lucidum spore powder wall recycle application range is expanded greatly.

Description

technical field [0001] The invention belongs to the field of biopharmaceuticals, and in particular relates to a ganoderma lucidum spore wall polysaccharide and an extraction method thereof. Background technique [0002] Ganoderma lucidum (Ganoderma lucidum) is a fungus of the genus Ganoderma lucidum of Polyporaceae, and it is a traditional precious medicinal material with the function of strengthening the body and strengthening the body. Ganoderma lucidum spore powder is extremely tiny oval germ cells ejected from the gills of Ganoderma lucidum at the mature stage of growth. With the development of Ganoderma lucidum cultivation technology, the output of Ganoderma lucidum spore powder is getting higher and higher, even exceeding the output of fruiting bodies. Polysaccharide is one of the main active ingredients in spore powder. In the past ten years, a lot of research has been done on the efficacy of Ganoderma lucidum spore powder, and it has been found that it has a variety...

Claims

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Application Information

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IPC IPC(8): C08B37/02
CPCC08B37/0024C08B37/0003
Inventor 徐靖李振皓李振宇王晓彤李明焱
Owner ZHEJIANG SHOUXIANGU PHARMA CO LTD
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