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Application of antibacterial peptide C-BF in preparation of medicines for preventing and treating weanling stress of piglets

A C-BF, piglet weaning technology, applied in the direction of drug combination, antidote, pharmaceutical formula, etc., can solve problems such as no one thought of research, and achieve the effect of regulating intestinal immune balance, reducing dosage, and alleviating drug resistance

Inactive Publication Date: 2015-05-27
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, no one has thought of studying antimicrobial peptides as a drug against weaning stress diarrhea in piglets

Method used

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  • Application of antibacterial peptide C-BF in preparation of medicines for preventing and treating weanling stress of piglets
  • Application of antibacterial peptide C-BF in preparation of medicines for preventing and treating weanling stress of piglets
  • Application of antibacterial peptide C-BF in preparation of medicines for preventing and treating weanling stress of piglets

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1, the preparation of antimicrobial peptide C-BF

[0020] Weigh 9 grams of sodium chloride, dissolve it in a small amount of distilled water, make up to 1000 ml with distilled water, and sterilize in an autoclave at 121° C. for 15 minutes to obtain sterilized physiological saline.

[0021] The synthesized antimicrobial peptide C-BF was prepared with the above-mentioned sterilized physiological saline, the concentration of the antimicrobial peptide C-BF was 0.2-1.0 mg / ml, filtered and sterilized with a 022 μm filter, and stored at 4°C until use.

Embodiment 2

[0022] Example 2: Effect of Antimicrobial Peptide C-BF on Growth Performance of Weaned Piglets

[0023] A total of 18 weaned piglets at the age of 21 days with the same genetic background and similar weight were randomly divided into two groups according to body weight and sex, with 3 pens in each group and 3 piglets in each pen. All piglets were weaned the morning before the experiment and fed a basal diet (without antibiotics). In the intraperitoneal injection group, one group received the same amount of normal saline, and the other group received the antimicrobial peptide C-BF, and the experimental period was 7 days. Follow the farm's routine piglet management procedures for immunization and deworming.

[0024] Table 1. Test groups

[0025]

[0026] The preparation of the basal ration in this experiment refers to the nutritional requirements of pigs in NRC (1998). The ration is full-price corn and soybean meal, and the basal ration does not contain zinc oxide and antib...

Embodiment 3

[0032] Example 3, the effect of antimicrobial peptide C-BF on the morphology of intestinal jejunum of weaned piglets

[0033] After the experiment, the animals were anesthetized by intramuscular injection of 4% pentobarbital sodium solution (40 mg / Kg), slaughtered and sampled. Carefully cut about 1.5 cm from the near-middle section of the jejunum, cut it lengthwise and spread it flat on filter paper, put it in a 50ml centrifuge tube of 4% paraformaldehyde, and store it at 4°C. H&E staining of intestinal tissue sections. The height of the jejunal villi and the depth of the crypt were measured, and the ratio of the height of the villi to the depth of the crypt was calculated.

[0034] The results are shown in Table 3.

[0035] Table 3. The effect of antimicrobial peptide C-BF on the jejunum morphology of weaned piglets

[0036]

[0037] As shown in Table 3, antimicrobial peptide C-BF increased the height of jejunal villi and decreased the depth of jejunal crypts, thereby i...

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Abstract

The invention discloses an application of an antibacterial peptide C-BF in preparation of medicines for preventing and treating weanling stress of piglets. The intestinal morphology can be improved by the antibacterial peptide C-BF; the villus height can be increased; the intestinal inflammation is relieved; the growth performance of weaned pigs is improved; the diarrhea rate is lowered; and the antibacterial peptide C-BF is dosed through intraperitoneal injection.

Description

technical field [0001] The invention provides an application and method of antimicrobial peptide C-BF in preventing and treating piglet weaning stress, which belongs to the technical field of animal husbandry and veterinary medicine. Background technique [0002] In modern animal husbandry production, piglets are generally weaned suddenly at 3-4 weeks (the sows are transferred away, and the piglets are transferred to the nursery). Weaning of piglets causes a strong intestinal stress response in piglets, damages the morphology and structure of the small intestinal epithelium, and reduces the transmembrane resistance of the epithelium, causing changes in the permeability of the small intestinal epithelium, and then antigens, toxins, and bacteria in the chyme enter the piglets through the intestinal epithelium Intestinal submucosal tissue induces a series of inflammatory reactions, leading to decreased feed intake and nutrient absorption of weaned piglets, resulting in stunted ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17A61P37/04A61P39/00A61P1/12A61P1/00
CPCA61K38/17
Inventor 汪以真易宏波
Owner ZHEJIANG UNIV
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