Bacillus zanthoxyli strain and microbial inoculum and application thereof

A technology of Bacillus prickly ash and Zanthoxylum bungeanum, applied in the direction of application, bacteria, biocide, etc., to achieve low cost, improve control effect, and improve effect

Active Publication Date: 2020-09-25
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is a lack of a Bacillus pepper strain isolated from pepper leaves that can produce volatile and nematode-attracting active ingredients and its bacterial agent and application

Method used

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  • Bacillus zanthoxyli strain and microbial inoculum and application thereof
  • Bacillus zanthoxyli strain and microbial inoculum and application thereof
  • Bacillus zanthoxyli strain and microbial inoculum and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0035] A strain of Bacillus zanthoxyli of the present invention, the strain is Bacillus zanthoxyli strain 1433; the preservation name is Bacillus zanthoxyli; it is preserved in the General Microbiology Center of the China Committee for the Preservation of Microorganisms, and the preservation address is Wuhan University, Wuhan, China; Date: June 14, 2018; Deposit number: CCTCC No.M 2018369.

[0036]The strain of Bacillus prickly ash is a gram-positive bacterium, cultured on a traditional beef extract peptone medium at 32° C. for 2 days, the colony diameter is 4 mm, pale yellow; the cells are round rod-shaped, 0.6 μm wide and 1.5 μm long , the cells are mobile, the strain grows in the temperature range of 4 °C, the optimum growth temperature is 32 °C; it grows in the range of pH 10.0, the optimum growth pH is 7.2; the highest concentration of NaCl tolerance is 7% ( W / V).

[0037] The Bacillus Zanthoxylum bacillus inoculum prepared by the Bacillus Zanthoxylum bacillus of the pre...

Embodiment 2

[0053] The difference between Example 2 and Example 1 is: the strain of Bacillus zanthani described in the present invention is a gram-positive bacteria, and is cultured for 2 days at 32°C on a traditional beef extract peptone medium, with a colony diameter of 2 mm and a pale yellow color. ; The cells are round rod-shaped, 0.8 μm wide and 1.3 μm long, and the cells are mobile. The strain grows in the temperature range of 45 °C, and the optimum growth temperature is 32 °C; it grows in the range of pH 6.0, and the optimum growth pH was 7.2; the highest concentration of NaCl tolerated was 7% (W / V).

[0054] The preparation method of the root-knot nematode attractant of the present invention comprises the following steps:

[0055] (1) (1) Bacillus zanthoxyli 1433 bacterial strain test tube slanted seed culture: the thalline of this bacterial strain is inoculated on the slanted surface of conventional beef extract peptone solid medium, and cultivated at 30°C for 2 days to obtain sl...

Embodiment 3

[0060] The difference between Example 3 and Example 1 is that: the Bacillus zantoniae strain of the present invention is a Gram-positive bacteria, and is cultured for 2 days at 32°C on a traditional beef extract peptone medium, with a colony diameter of 5 mm and a pale yellow color. ; The cells are rod-shaped, with a width of 0.9 μm and a length of 1.2 μm, and the cells are mobile. The strain grows in the temperature range of 40 °C, and the optimum growth temperature is 32 °C; it grows in the range of pH 10.0, and the optimum growth pH was 7.2; the highest concentration of NaCl tolerated was 7% (W / V).

[0061] The preparation method of the root-knot nematode attractant of the present invention comprises the following steps:

[0062] (1) (1) Bacillus zanthoxyli 1433 bacterial strain test tube slanted seed culture: the thalline of this bacterial strain is inoculated on the slanted surface of conventional beef extract peptone solid medium, and cultivated at 37° C. for 3 days to o...

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Abstract

The invention discloses a Bacillus zanthoxyli strain and a microbial inoculum and an application thereof. The Bacillus zanthoxyli strain is a Bacillus zanthoxyli 1433 strain; the preservation name ofthe strain is Bacillus zanthoxyli 1433; the strain is preserved in China Center for Type Culture Collection, and the preservation address is Wuhan University, Wuhan, China. The preservation date is June 14, 2018; the preservation number of the strain is CCTCC No. M 2018369. The root-knot nematode attractant provided by the invention has the characteristics of low cost, no residue and safety to human and livestock, and can obviously enhance the control effect of the conventional nematode-killing pesticide.

Description

technical field [0001] The invention relates to the technical field of plant disease prevention and control, in particular to a Bacillus zanthoxyli 1433 strain and a fungicide thereof, and its application in preventing and treating plant root-knot nematode disease. Background technique [0002] Plant-parasitic nematodes are one of the major pathogens threatening agricultural production, causing economic losses of up to $157 billion annually worldwide (Elling, 2013). my country is located in the subtropical zone, with complex farming systems, high multi-cropping index, various plant species, and serious occurrence and damage of various nematodes, especially Meloidogyne spp. The economic losses suffered by crops due to root-knot nematode damage each year amount to more than hundreds of millions of yuan (Ye et al., 2019). Plant root cells are induced to proliferate into giant cells, which in turn block the ducts for plants to absorb nutrients and water, causing symptoms such as ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P19/00A01P5/00G01N30/02C12R1/07
CPCC12N1/20A01N63/22G01N30/02C12R2001/07C12N1/205
Inventor 马莉陈佳清莫明和
Owner YUNNAN UNIV
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