Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for establishing PDX model of chronic myeloid leukemia

A chronic granulocyte and establishment method technology, applied in the field of PDX model establishment, can solve the problems of lack of effective treatment methods and achieve good application prospects

Active Publication Date: 2020-09-29
SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE +1
View PDF11 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Unfortunately, due to the lack of effective treatment for the blast phase, the median survival time of patients with myeloid blast is only 6 months, and that of lymphoid blast is about 12 months.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for establishing PDX model of chronic myeloid leukemia
  • Method for establishing PDX model of chronic myeloid leukemia
  • Method for establishing PDX model of chronic myeloid leukemia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] 1. Sample processing of CML patients:

[0053] 1. Collect 10-25 mL of bone marrow from the patient, centrifuge with Ficoll separation medium, and separate mononuclear cells from the patient. After washing the mononuclear cells twice with normal saline, resuspend in 0.5 mL of normal saline, and count the cells.

[0054] 2. For patients with blast phase, CD34 + Sorting: (2) Add rabbit anti-human CD34 antibody to the cells in step (1), incubate on ice for 20 minutes, wash excess antibody with PBS, resuspend in 0.5mL PBS, and sort out CD34 by flow cytometry + Hematopoietic stem / progenitor cells; the sorted cells were counted, resuspended in 0.2-1mL PBS, and injected into mice through the tail vein, and the number of inoculated cells was 1×10 6 / Only.

[0055] 2. Feeding and inoculation of NSG mice:

[0056] 1. Raising conditions for mice: 8-week-old female NSG mice were raised under SPF grade conditions. The room temperature is 18-25°C, the relative humidity is 40%-60%,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for establishing a PDX model of chronic myeloid leukemia, and relates to the technical field of animal models. The method specifically comprises the steps of: screening CML clinic patients in a chronic phase and an acute transformation phase, separating mononuclear cells in bone marrow, transplanting the mononuclear cells into an NSG mouse radiated by a half lethaldose, and carrying out CD34 marking before transplantation aiming at the acute transformation phase so as to screen stem / progenitor cells. A user can judge whether the model is successfully established according to the implantation ratio of human-derived cells in peripheral blood after 4-16 weeks of the transplantation. Tumor growth characteristics and cytogenetic abnormality of a series of established CML PDX mouse models are same as those of sourcing patients, so that an important preclinical study model is provided for researching pathogenesis and convulsion mechanisms of CML and screeningtargets and small molecular drugs aiming at different stages of the CML, and the method has relatively good popularization and application values.

Description

technical field [0001] The invention belongs to the technical field of animal models, and in particular relates to a method for establishing a PDX model of chronic myelogenous leukemia. Background technique [0002] Chronic myeloid leukemia (CML) is a malignant tumor of the hematopoietic system that originates from hematopoietic stem cells and is characterized by the extreme proliferation of myeloblasts, accounting for about 15% of the total number of leukemia patients. The main clinical manifestations are anemia, extreme myeloblastosis and hepatosplenomegaly. CML has distinct cytogenetic characteristics, and 90% of the bone marrow cells contain Philadelphia (Ph) chromosome, that is, t: (9,22) (q34.1; q11) chromosome translocation. This translocation fuses the ABL gene located on chromosome 9 with the BCR gene located on chromosome 22 to express the BCR-ABL fusion protein. The fusion protein BCR-ABL has continuously activated tyrosine kinase activity, and promotes cell pro...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02
CPCA01K67/02A01K2227/105A01K2267/0381A01K2207/12
Inventor 卢莹闫金松江月
Owner SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com