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Kit for simultaneously detecting four tumor markers of lung cancer and application

A tumor marker and kit technology, applied in the field of immunodetection, can solve the problems of low specificity, sensitivity and accuracy, increasing the difficulty of research and development, and high cost

Pending Publication Date: 2020-10-16
万东山
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional detection of four tumor markers is a separate determination of each tumor marker, such as the published Chinese patent application CN106483298B "Chemiluminescence immunoassay kit for detecting cytokeratin 19 fragments" and CN102914650B "neuron Specific Enolase Quantitative Detection Kit and Its Preparation Method and Application”, etc., the above method is complicated to operate, high in cost, specificity, sensitivity and accuracy are not too high, and has limited value for clinical diagnosis
[0008] Neuron-specific enolase (NSE), cytokeratin fragment 19 (CYFRA21-1), gastrin-releasing peptide precursor (ProGRP), squamous cell carcinoma antigen (SCC) combined detection of four tumor markers, Although it is theoretically possible to distinguish between small cell carcinoma (SCLC) and non-small cell carcinoma (NSCLC), in practice, the increase in the number of markers undoubtedly increases the difficulty of research and development

Method used

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  • Kit for simultaneously detecting four tumor markers of lung cancer and application
  • Kit for simultaneously detecting four tumor markers of lung cancer and application
  • Kit for simultaneously detecting four tumor markers of lung cancer and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Example 1 Preparation of magnetic particles labeled with NSE monoclonal antibody and acridinium ester coupled with NSE monoclonal antibody

[0073] In the preliminary research process of the present invention, monoclonal antibodies targeting different sites of the NSE antigen were used for preliminary screening and optimization. After the research of the present invention, if four tumor markers are to be detected simultaneously, monoclonal antibodies targeting different sites of the NSE antigen must be used Combination (available from sigma, product number AMAB90556, HPA007007), after testing, the above NSE monoclonal antibody has no cross-reaction with CYFRA21-1, ProGRP, SCC antibody, although it is not the monoclonal antibody with the highest titer in the test, but it is detected at the same time The accuracy is the best among them, so NSE monoclonal antibodies at different sites were used to prepare labeled magnetic particles and acridinium ester conjugates. The spec...

Embodiment 2

[0094] Example 2 Preparation of magnetic particles labeled with CYFRA21-1 monoclonal antibody and acridinium ester coupled with CYFRA21-1 monoclonal antibody

[0095] In the preliminary research process of the present invention, monoclonal antibodies targeting different sites of the CYFRA21-1 antigen were used for preliminary screening and optimization. After the research of the present invention, if four tumor antigens are to be detected simultaneously, it is necessary to use monoclonal antibodies targeting different sites of the CYFRA21-1 antigen. Monoclonal antibody combinations (purchased from Feipeng Biotechnology Co., Ltd., the product numbers are CY211-McAb1#, CY211-McAb2#, respectively). After testing, the above-mentioned CYFRA21-1 monoclonal antibody has no cross-reaction with NSE, ProGRP, and SCC antibodies, and has the best accuracy in simultaneous detection, so the present invention uses CYFRA21-1 monoclonal antibodies at different sites to prepare labeled magnetic ...

Embodiment 3

[0116] Example 3 Preparation of magnetic particles labeled with ProGRP monoclonal antibody and acridinium ester coupled with ProGRP monoclonal antibody

[0117] In the preliminary research process of the present invention, monoclonal antibodies directed at different sites of ProGRP antigen are used for preliminary screening and optimization. After the research of the present invention, if four tumor markers are to be detected simultaneously, a combination of monoclonal antibodies at different sites of ProGRP is required ( Bought from Faipeng Biological Co., Ltd., the product numbers are GRP-McAb1 and GRP-McAb2). After testing, the above-mentioned GRP-McAb monoclonal antibody has no cross-reaction with CYFRA21-1, NSE, and SCC antibodies, and the accuracy is the best in simultaneous detection, so the present invention uses ProGRP monoclonal antibodies at different sites to prepare labeled magnetic Microparticles and acridinium ester conjugates. The specific method is as follows...

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Abstract

The invention belongs to the technical field of immunodetection, and particularly relates to a kit for simultaneously detecting four tumor markers of lung cancer and application. The invention provides a kit for simultaneously detecting four tumor markers of lung cancer. The kit adopts a magnetic particle chemiluminiscence method to simultaneously detect four tumor markers, namely neuronal specific enolase, a cytokeratin fragment 19, a gastrin releasing peptide precursor and squamous cell carcinoma antigens, of lung cancer. The kit has the advantages of high sensitivity, high precision, good reliability, difficulty in being influenced by human operation factors, capability of simultaneously detecting four tumor markers, convenience and quickness in operation, convenience in standardizationin operation and capability of being used for laboratory detection, field detection and the like.

Description

technical field [0001] The invention belongs to the technical field of immune detection, and in particular relates to a kit and application for simultaneously detecting four tumor markers of lung cancer. Background technique [0002] Lung cancer prevention strategies are very important to reduce lung cancer mortality. It is very important to strengthen the screening of high-risk groups and promote the physical examination of healthy people. Lung cancer is mainly divided into small cell carcinoma (SCLC) and non-small cell carcinoma (NSCLC). Small cell lung cancer is a highly malignant tumor with bad biological behavior and dire prognosis. Compared with other types of lung cancer, small cell lung cancer has a shorter symptom period before diagnosis and a shorter survival period after diagnosis than other types of lung cancer. One third of lung cancer patients belong to this type. Non-small cell lung cancer, including squamous cell carcinoma, adenocarcinoma, and large cell ca...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/543
CPCG01N33/57484G01N33/57423G01N33/54326
Inventor 万东山
Owner 万东山