PCR reagent for detecting gene expression of cell angiogenesis signal path and application thereof

An angiogenesis and gene technology, applied in the field of molecular biology, can solve the problems of excessive growth and change of tumor blood vessels

Pending Publication Date: 2020-10-30
THE AFFILIATED HOSPITAL OF QINGDAO UNIV
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In normal tissues, the switch of angiogenesis is turned off due to the lack of angiogenesis factors or the strict control of high levels of angiogenesis inhibitors, but in tumor tissues, due ...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • PCR reagent for detecting gene expression of cell angiogenesis signal path and application thereof
  • PCR reagent for detecting gene expression of cell angiogenesis signal path and application thereof
  • PCR reagent for detecting gene expression of cell angiogenesis signal path and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0289] The present invention is illustrated by human liver cancer Huh7 and normal liver cell liver cell, but the present invention is not limited to these two types of cells. The PCR detection reagent of the present invention can be applied to common cancers including breast cancer, lung cancer, gastric cancer and the like.

[0290] The invention cultivates two cell lines: human liver cancer Huh7 and normal liver cell liver cell, and then detects the changes of core angiogenesis molecules in the two cell lines by using an angiogenesis signal pathway detection reagent.

[0291] The human liver cancer Huh7 used in the present invention and normal liver cell liver cells were purchased from ATCC Company of the United States, Shanghai Cell Bank, DMEM medium, and fetal bovine serum were purchased from Gibco Company of the United States, RNAiso for extracting total RNA, reverse transcription kit, Premix Ex Taq kit was purchased from TaKaRa.

[0292] The detection of the intracellula...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a PCR reagent for detecting gene expression of an angiogenesis signal channel and an application thereof. The PCR reagent comprises PCR reaction primers for detecting AKT1 genes, ANG genes, ANGPT1 genes, ANGPT2 genes and other related genes, reference genes and the like. According to the invention, angiogenesis-related signal molecules are concentrated on a flat plate; a real-time fluorescent quantitative PCR reaction is carried out to reflect the survival state of cells, human liver cancer Huh7 is compared with normal liver cells, possible ways of an angiogenesis signalpath in the human liver cancer and the normal liver cells are discussed, and most direct evidences are provided for research on regulation and control of key proteins; according to the invention, molecules related to the angiogenesis signal pathway are rapidly and accurately found from the transcriptional level, and a powerful tool is provided for mechanism discussion of new targeted drugs, development of angiogenesis related inhibitors and the like.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to a PCR reagent for detecting gene expression of angiogenesis signal pathway of cells and application thereof. Background technique [0002] The angiogenesis signaling pathway is an extremely important signal transduction pathway in living organisms. In recent years, one of the major advances in tumor research is the establishment of the important position of tumor angiogenesis in tumor development and the significance of anti-angiogenesis drug treatment. People try to kill cancer cells by cutting off the tumor's blood supply. Since in normal adults, except for wound healing and reproductive cycle, almost all angiogenesis is pathological, therefore, selective inhibition of angiogenesis has its special advantages for tumor therapy. [0003] Tumor angiogenesis is similar to normal angiogenesis, but because it is in the special microenvironment of tumor, its particularity...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/6886C12Q1/6851C12N15/11
CPCC12Q1/6886C12Q1/6851C12Q2600/158C12Q2600/136C12Q2561/113C12Q2563/107C12Q2545/101C12Q2531/113
Inventor 刘世海贺桂芳刘畅畅蔡铎潘华政
Owner THE AFFILIATED HOSPITAL OF QINGDAO UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap