PCR reagent for detecting gene expression of cell angiogenesis signal path and application thereof

Abstract

The invention provides a PCR reagent for detecting gene expression of an angiogenesis signal channel and an application thereof. The PCR reagent comprises PCR reaction primers for detecting AKT1 genes, ANG genes, ANGPT1 genes, ANGPT2 genes and other related genes, reference genes and the like. According to the invention, angiogenesis-related signal molecules are concentrated on a flat plate; a real-time fluorescent quantitative PCR reaction is carried out to reflect the survival state of cells, human liver cancer Huh7 is compared with normal liver cells, possible ways of an angiogenesis signalpath in the human liver cancer and the normal liver cells are discussed, and most direct evidences are provided for research on regulation and control of key proteins; according to the invention, molecules related to the angiogenesis signal pathway are rapidly and accurately found from the transcriptional level, and a powerful tool is provided for mechanism discussion of new targeted drugs, development of angiogenesis related inhibitors and the like.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to a PCR reagent for detecting gene expression of angiogenesis signal pathway of cells and application thereof. Background technique [0002] The angiogenesis signaling pathway is an extremely important signal transduction pathway in living organisms. In recent years, one of the major advances in tumor research is the establishment of the important position of tumor angiogenesis in tumor development and the significance of anti-angiogenesis drug treatment. People try to kill cancer cells by cutting off the tumor's blood supply. Since in normal adults, except for wound healing and reproductive cycle, almost all angiogenesis is pathological, therefore, selective inhibition of angiogenesis has its special advantages for tumor therapy. [0003] Tumor angiogenesis is similar to normal angiogenesis, but because it is in the special microenvironment of tumor, its particularity...

AI Technical Summary

Problems solved by technology

In normal tissues, the switch of angiogenesis is turned off due to the lack of angiogenesis factors or the strict control of high levels of angiogenesis inhibitors, but in tumor tissues, due to the excessive expression of growth factors, the expression of inhibitors is too low, Alters this balance so that tumor angiogenesis is kept switched on, causing overgrowth of tumor blood vessels

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