Molecular marker influencing chicken shin skin color and application of molecular marker
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A technology of molecular marking and skin color, which is applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of inability to identify skin color, reduce breeding time, promote sales, and increase sales Effect
Active Publication Date: 2020-11-03
SOUTH CHINA AGRI UNIV
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[0004] Patent CN201010531819.2 provides a molecular marker related to chicken skin color and its identification method
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Embodiment 1
[0036] Example 1 Primer Design
[0037] 1. Experimental method
[0038] Find the sequence of the 3' non-coding region of the ALDH1A3 gene from https: / / www.ncbi.nlm.nih.gov / , and design a primer pair. The primer pair information is shown in Table 1 (primer sequence 5'→3'). The sequence of the primer pair was sent to Guangzhou Sangon Biotech Co., Ltd. for synthesis, and the primer pair was used to perform PCR on tissue DNA to test the specificity of the primers.
[0039] Table 1 Primer information for SNP screening of ALDH1A3
[0040]
[0041] 2. Experimental results
[0042] The results are attached figure 1 shown. Use this primer pair to carry out PCR on blood DNA, as attached figure 1 As shown, the primers are specific
Embodiment 2
[0043] Example 2 DNA pool sequencing
[0044] 1. Experimental method
[0045] The sample chickens were selected from 600 120-day-old Nanhai Jute Chicken No. 1 pure line of Jiangfeng Chicken Farm. All chickens were hatched in the same batch, raised in a flat way, and fed with corn-soybean meal that meets international formula standards feed. Use a 2ml syringe to collect blood from the subwing vein, collect the blood and store it in a centrifuge tube containing 20% EDTA for blood DNA extraction.
[0046] Genomic DNA of all individuals based on Plant Mini Kit (Qiagen, Hilden, CA; Cat#69104) was used in the kit instructions to extract blood DNA, measure the quality and concentration, dilute to 50 ng / μL, and store at 4°C for later use.
[0047] 30 DNA samples were randomly selected from the total extracted DNA samples to construct a mixed pool, and every three samples (about 0.33 μL of each sample) were mixed into a mixed sample, a total of 10 samples. The mixed pool samples ...
Embodiment 3
[0050] Example 3 Determination of genotype determination of ALDH1A3 mutation site g.703T>C
[0051] 1. Experimental method
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Abstract
The invention discloses a molecular marker influencing chicken shin skin color and an application of the molecular marker, and an application of a chicken ALDH1A3 gene 3'non-coding region sequence g.170A > G molecular marker as a detection site for identifying chicken shin skin color, the shin epidermis yellowness of an AA genotype individual is greater than that of an AG genotype individual and that of a GG genotype individual. The molecular marker is used for marker-assisted selection, the yellowness of the shin skin after chicken is slaughtered can be greatly deepened, and the uniformity ofthe yellowness of the shin skin of chicken flocks is increased. By means of the molecular marker and a primer pair, an efficient and accurate molecular marker assisted breeding technology can be established, and the molecular marker assisted breeding technology is applied to genetic improvement of the shin skin yellowness character after the chicken is slaughtered, so that the shin skin yellowness after the chicken is slaughtered is deepened, the uniformity of the shin skin yellowness of chicken flocks is improved, and the sales volume of chickens, especially chicken feet, is promoted; The sales amount of enterprises is increased, and the core competitiveness is improved.
Description
technical field [0001] The invention relates to the technical field of molecular breeding, in particular to a molecular marker affecting the skin color of chicken shanks and an application thereof. Background technique [0002] Chicken is a kind of poultry, which is the main edible poultry in our country. Consumers largely determine how chickens are bred and sold. For example, in southern China, whole chickens are mainly sold, while in northern China, they are mainly sold in splits. People in different regions have different preferences for broiler skin color, but the golden color of broiler chicken is considered to be closely related to the quality of meat. Consumers are more inclined to buy broiler chickens with good appearance (yellow skin) and consistent appearance characteristics. Due to several outbreaks of bird flu, the sales of fresh chickens have been restricted, and their sales have gradually declined, while the market for chilled chickens has gradually expanded,...
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