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A kind of hot spring cyanobacteria with high temperature tolerance and high carbohydrate yield and its application

A carbohydrate and high-temperature tolerance technology, applied in the direction of bacteria, microorganism-based methods, microorganisms, etc., can solve problems such as research lag, and achieve the effect of wide tolerance range, strong adaptability, and high-speed growth

Active Publication Date: 2022-04-08
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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Problems solved by technology

However, due to the relatively lagging research on the isolation and purification of hot spring microorganisms, the development of "temperature-type" microalgae resources in terrestrial hot springs still has room for development

Method used

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  • A kind of hot spring cyanobacteria with high temperature tolerance and high carbohydrate yield and its application
  • A kind of hot spring cyanobacteria with high temperature tolerance and high carbohydrate yield and its application
  • A kind of hot spring cyanobacteria with high temperature tolerance and high carbohydrate yield and its application

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Embodiment Construction

[0027] The principles and features of the present invention are described below in conjunction with examples, which are only used to explain the present invention and are not intended to limit the scope of the present invention.

[0028] 1. Acquisition of Thermophylla NK1-22

[0029] The algae strain (Thermoleptolyngbya sp. NK1-22) of the present invention is isolated from a biological mat sample at the mouth of a hot spring in Yunnan. The cell morphology was observed by light microscope and scanning electron microscope. figure 1 As shown, multiple cells strung together into a single row of cell filaments, blue-green in color, without nuclei, the filaments are of equal width up and down, and there are obvious colloidal sheaths outside, which is a typical shape of Thermophyllum spp. Genomic DNA was extracted to amplify the 16S rRNA gene sequence, as shown in SEQ ID NO: 1, compared with the microalgal 16S rRNA gene in the database, and a phylogenetic tree was constructed, showi...

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Abstract

The present invention relates to a high-yield carbohydrate-producing Coelophora NK1-22, which was preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee on December 27, 2019, and the preservation number is CGMCC NO .19155; also relates to the application of the Codella thermocellarum in the production of carbohydrates; also relates to a method of cultivating the above Cod. The Codella thermocellus of the present invention has a soluble sugar content of 57%, and can secrete a higher concentration of soluble sugar into the culture environment. Although the algae strain is isolated from the hot spring environment, it has a very wide temperature tolerance range. It grows well in both the conventional environment of 30°C and the high temperature environment of 50°C. It provides high temperature and high light intensity during cultivation, which can not only maintain high-speed growth, but also reduce the possibility of polluting algae and bacteria.

Description

technical field [0001] The invention relates to the field of applied microalgae, and more particularly relates to a hot spring cyanobacterium with high temperature tolerance and high carbohydrate yield and its application. Background technique [0002] Microalgae biotechnology can get rid of the predicament of "competing with people for food" and "competing with food for land" in traditional green chemical industry, and it is a photosynthetic biosynthesis route that has attracted much attention. However, with the rapid development of microalgae large-scale cultivation technology, the bottleneck problems that have not been discovered or estimated in laboratories or Chinese scales such as microorganisms, viruses, and protozoa contamination have become increasingly prominent. Breeding high-quality algae species, discovering new cultivation techniques, and developing high-quality products that meet the needs of the national economy and the people's livelihood are urgent problems...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P19/02C12P19/12C12R1/01
CPCC12N1/20C12P19/02C12P19/12C12R2001/01C12N1/205
Inventor 吕雪峰朱涛尼丁·克什李李辉谢玉曼齐凤霞
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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