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Sensor for detecting acetamiprid based on electrochemical luminescence method as well as preparation method and application

An acetamiprid and electrochemical technology, which is applied in the field of sensors for detecting acetamiprid based on electrochemiluminescence and its preparation and application, can solve the problems of low sensitivity, complicated operation, high cost, etc., and achieve wide detection range and high sensitivity , the effect of low detection limit

Active Publication Date: 2020-12-18
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods are complicated to operate, time-consuming, high cost, and the sensitivity of simultaneous detection is not high

Method used

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  • Sensor for detecting acetamiprid based on electrochemical luminescence method as well as preparation method and application
  • Sensor for detecting acetamiprid based on electrochemical luminescence method as well as preparation method and application
  • Sensor for detecting acetamiprid based on electrochemical luminescence method as well as preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0034] (1)MoS 2 Preparation of QDs-PATP@PTCA composites:

[0035] 0.25g of Na 2 MoO 4 2H 2 O was dissolved in 20 mL of water and adjusted to pH 6.5 with 0.1 M HCl. After sonication for 10 min, 0.5 g of cysteine ​​and 40 mL of deionized water were added. The above mixture was transferred to a 100 mL polytetrafluoroethylene autoclave, reacted at 200 °C for 36 h, cooled naturally to room temperature, centrifuged at 10000 rpm for 20 min, and the supernatant was taken and stored at 4 °C. Dissolve 125 mg of 4-aminothiophenol (PATP) in 10 mL of ethanol solution, and inject it into 10 mL of fluorescent molybdenum disulfide quantum dots (MoS 2 QDs) solution, stirred for 6 h in the dark. Washed with ethanol, centrifuged, separated, and the final MoS 2 QDs-PATP was dispersed in ethanol (1 mg / mL).

[0036] Dissolve 0.1g of perylenetetracarboxylic dianhydride (PTCDA) in 10mL of 0.1M NaOH, stir at 80°C for 2h, after obtaining a yellow-green solution, add 1.0M HCl dropwise until comp...

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Abstract

The invention provides a method for detecting acetamiprid, and particularly belongs to the field of electrochemical luminescence detection. The operation process comprises the following steps: (1) preparing a composite material of MoS2QDs-PATP@PTCA and NH2-UiO-66; (2) preparing an electrochemical luminescence sensor; and (3) detecting acetamiprid by using an electrochemical luminescence method. Aglassy carbon electrode modified by NH2-UiO-66-pDNA/apt/MoS2QDs-PATP @ PTCA/GCE is used as a working electrode, an Ag/AgCl electrode is used as a reference electrode, and a platinum electrode is usedas an auxiliary electrode to form a traditional three-electrode system. The detection range of the method is 1.0 * 10-7mol/L-1. 0 * 10-18mol/L, and the lowest detection limit is 6.4 * 10-19mol/L. Themethod for detecting acetamiprid is low in cost, high in sensitivity, strong in specificity and simple to operate.

Description

technical field [0001] The invention relates to an electrochemiluminescent method for detecting acetamiprid, in particular to a method for immobilizing molybdenum sulfide quantum dots (MoS) with perylenetetracarboxylic acid (PTCA). 2 QDs-PATP), then the MoS 2 QDs-PATP@PTCA as a donor in energy resonance transfer, NH 2 -UiO-66 as an acceptor in energy resonance transfer. The donor and the acceptor are connected by two DNA strands (apt, pDNA), and they are co-modified on the glassy carbon electrode (GCE). The aptamer (apt) with specific recognition function is used as the recognition element, and then NH 2 -UiO-66-pDNA / apt / MoS 2 QDs-PATP@PTCA / GCE is used as the working electrode to quantitatively detect acetamiprid in water by electrochemiluminescence analysis. Background technique [0002] Acetamiprid is a new type of neonicotinoid insecticide, which is widely used in pest control of agricultural products. It is also potentially toxic to the nervous system and reproductiv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N21/76G01N27/48
CPCG01N27/308G01N27/48G01N21/76
Inventor 陈智栋李静娴单学凌蒋鼎王文昌
Owner CHANGZHOU UNIV
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