Solution adding device for cell culture

Abstract

The invention discloses a solution adding device for cell culture. The solution adding device comprises a solution adding cylinder and a shell located outside the solution adding cylinder, wherein a one-way outlet and a one-way solution inlet are formed in the bottom of the solution adding cylinder, a solution adding piston sliding in a sealed mode is arranged in the solution adding cylinder, a push rod cylinder is arranged on the outer side of the solution adding piston, an air adding chamber is formed in the push rod cylinder, the solution adding piston is provided with a one-way channel which communicates with the air adding chamber and the solution adding cylinder in a one-way mode, a one-way air inlet is formed in the bottom of the push rod cylinder, and an air adding piston which slides in a sealed mode is arranged in the push rod cylinder, a movable locking ring is arranged on the push rod cylinder, when the air adding piston moves to the top of the air adding chamber, the relative positions of the push rod cylinder and the air adding piston are locked by the movable locking ring, and when the solution adding piston moves to the bottom of the solution adding cylinder, the push rod cylinder and the air adding piston are unlocked by the movable locking ring. The solution adding device aims to prevent a nutrient solution from remaining at the outlet and shorten the cleaningand maintenance time of operators.

Description

technical field [0001] The invention belongs to the field of cell culture, in particular to a liquid adding device for cell culture. Background technique [0002] During the cell culture process, it is necessary to regularly add nutrient solution to the culture device to maintain the concentration of the culture solution. The filling of nutrient solution usually uses equipment such as a cell culture machine to add liquid through a syringe. However, after each filling of the syringe, there will often be some nutrient solution remaining at the end of the pipeline, and the remaining part of the nutrient solution will continue to fall, thereby contaminating the culture. Inside the machine equipment, operators need to clean and maintain the equipment frequently, resulting in unnecessary waste of working hours. Contents of the invention [0003] In order to overcome the shortage of internal pollution caused by residual nutrient solution after adding liquid to the cell culture m...

AI Technical Summary

Problems solved by technology

[0003] In order to overcome the shortage of internal pollution caused by residual nutrient solution in the cell culture machine after adding liquid in the prior art, the present invention provides a liquid adding device for cell culture, which can avoid residual nutrient solution at the outlet and reduce cleaning and maintenance by operators time

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