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SNPs for identifying oxyeleotris fingerlings, AS-PCR primer group based on SNPs, detection method and application thereof

A detection method, the technology of Channa cuspidatum, applied in the field of AS-PCR primer set and its detection, can solve the problems that there is no SNP-related technology application, and achieve the effect of good application prospects

Active Publication Date: 2021-02-23
PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI +1
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  • Abstract
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Problems solved by technology

However, there is no application of SNP-related technology in the identification of Channa japonicus species

Method used

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  • SNPs for identifying oxyeleotris fingerlings, AS-PCR primer group based on SNPs, detection method and application thereof
  • SNPs for identifying oxyeleotris fingerlings, AS-PCR primer group based on SNPs, detection method and application thereof
  • SNPs for identifying oxyeleotris fingerlings, AS-PCR primer group based on SNPs, detection method and application thereof

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Embodiment Construction

[0091] In order to make the object, technical solution and technical effect of the present invention clearer, the present invention will be further described in detail below in conjunction with specific embodiments. It should be understood that the specific implementations described in this specification are only for explaining the present invention, not for limiting the present invention.

[0092] The experimental materials and reagents used, unless otherwise specified, are conventional consumables and reagents that can be obtained from commercial channels.

[0093] The test equipment used in the following examples includes a UV spectrophotometer (type AG22331 from Eppendorf).

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Abstract

The invention discloses SNPs for identifying oxyeleotris fingerlings, an AS-PCR primer group based on the SNPs, a detection method and application thereof. An SNP marker is located in a sequence shownin SEQ ID NO. 1. According to the invention, the obtained SNPs locus is converted into the AS-PCR marker, and the AS-PCR is utilized to identify three kinds of oxyeleotris. The detection method provided by the invention has the advantages of simple operation, short time consumption and low cost. The designed AS-PCR primer group has high sensitivity, the P2 primer can be diluted to 10-4 times in atemplate, and the P3 primer can be diluted to 10-3 times in the template, so that the primer group has extremely high application value.

Description

technical field [0001] The invention belongs to the field of DNA marker technology and application of animal molecular biology, and in particular relates to SNPs for identifying snakehead species, an AS-PCR primer set based on SNPs, a detection method and application thereof. Background technique [0002] Oxyeleotris marmoratus and Oxyeleotris lineolatus belong to Perciformes, Gobioidei, Eleotridae, and Oxyeleotris lineolatus taxonomically. Channa (Oxyeleotris) are famous and high-quality fish imported from abroad in China. [0003] The cloud-spotted sharp pond snakehead, English name marble goby, commonly known as Thai bamboo shoot shell fish, is native to Thailand, Vietnam and other Southeast Asian countries. Its body surface is yellowish brown, with obvious markings, beautiful appearance, high market price, but the breeding process Minor phenomena are serious and affect the survival rate of cultivation. The lined snakehead, the English name is Sleepy cod, commonly known...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6858C12Q1/6888C12Q2600/156C12Q2600/172C12Q2531/113C12Q2527/143C12Q2565/125C12Q2535/125
Inventor 樊佳佳马冬梅朱华平林明辉池金泉
Owner PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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