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Glutamine, and method for simultaneously detecting glutamine and asparagine in sample

A determination method and technology of glutamine, which are applied in measurement devices, biological tests, material inspection products, etc., can solve the problems of high price, unstable results, poor accuracy and stability of measurement results, etc., to ensure normal operation and save money. time, the effect of improving accuracy and stability

Pending Publication Date: 2021-03-19
FUJIAN AONONG BIOLOGICAL TECH GRP CO LTD +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are several methods for the detection of the two: 1. Gene sequencing, which is responsible and expensive, and can only measure the glutamine content of pure protein, which is not suitable for routine detection of daily samples and enterprises
2. Enzyme hydrolysis, because the related enzyme product technology is relatively immature, the quality and stability of the enzyme will have a huge impact on the results, and the detection results are also unstable
[0005] The use of highly toxic reagents in the measurement process takes a long time, and the accuracy and stability of the measurement results are poor

Method used

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  • Glutamine, and method for simultaneously detecting glutamine and asparagine in sample

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] The instrument and instrument condition that present embodiment adopts are as follows:

[0083] 1. Instruments and equipment

[0084] Amino acid analyzer, electronic balance (accurate to 0.1mg), laboratory glassware, ultrasonic cleaner, 0.22μm microporous filter membrane, solvent filter, high-speed centrifuge.

[0085] The specific operation is as follows:

[0086] 1. Accurately weigh 0.1g of concentrated feed containing both glutamic acid and aspartic acid into a 35mL high-pressure reaction tube, add 1.5mL of ethanol solution containing 10mg / mL BTI and 1mL of pH value of 3.2 Sodium citrate buffer solution, vortexed to mix, and ultrasonically reacted in a 60°C water bath for 1h.

[0087] 2. After the reaction is completed, add 15mL of 6mol / L hydrochloric acid and react at 130°C for 2h, take it out and cool it.

[0088] 3. After cooling, transfer to a 50mL volumetric flask with water to make up to the mark, and after filtration, accurately pipette 0.5mL of the solutio...

Embodiment 2

[0098] The instrument and instrument condition that present embodiment adopts are as follows:

[0099] 1. Instruments and equipment

[0100] Amino acid analyzer, electronic balance (accurate to 0.1mg), laboratory glassware, ultrasonic cleaner, 0.22μm microporous filter membrane, solvent filter, high-speed centrifuge.

[0101] A method for the simultaneous detection of glutamine and asparagine in a sample:

[0102] 1. Accurately weigh 0.1g of potato protein powder into a 35mL high-pressure reaction tube, add 1mL of ethanol solution containing 15mg / mL BTI and 2mL of sodium citrate buffer solution with a pH of 3.0. After vortex mixing, ultrasonically react in a 50°C water bath for 1.5h.

[0103] 2. After the reaction is completed, add 15mL of 6mol / L hydrochloric acid and react at 120°C for 4h. Remove to cool.

[0104] 3. After cooling, transfer to a 50mL volumetric flask with water to make up to the mark, and after filtration, accurately pipette 0.5mL of the solution and plac...

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Abstract

The invention discloses a glutamine, and a method for simultaneously detecting glutamine and asparagine in a sample, and belongs to the technical field of chemical component determination. The methodcomprises the following steps: detecting glutamic acid contents of a to-be-detected sample group and a control group thereof; mixing and reacting a to-be-detected sample with a BTI-dissolved ethanol solution and a citrate buffer solution, reacting with the first acid solution, and determining the glutamic acid content C obtained after the reaction; wherein the ethanol solution in the to-be-detected sample control group does not contain BTI, and the obtained glutamic acid content is recorded as C0; and obtaining the content X of glutamine in the to-be-detected sample according to X = (C0-C) / F,wherein F is a correction coefficient for correcting the determination results of the to-be-detected sample group and the to-be-detected sample control group. According to the method, use of toxic reagents is avoided, nitrogen blowing treatment is not needed, the determination time is saved, the determination result obtained according to the method is high in accuracy and stable, it is guaranteedthat the feed can reach the standard content, it is guaranteed that the feed is balanced in nutrition, and the animal growth requirement is met.

Description

technical field [0001] The invention relates to the technical field of chemical composition determination, in particular to a glutamine and a method for simultaneously determining glutamine and asparagine in a sample. Background technique [0002] Glutamine is an amino acid naturally present in various proteins, although it is not an essential amino acid for humans or animals. [0003] However, research in recent years has found that it has a positive effect on the intestinal health of animals. In the face of the current trend of animal feed without antibiotics, it can be said that glutamine is a product with great potential for replacing antibiotics. Similarly, asparagine also plays an important role in feed. [0004] At present, there are several methods for the detection of the two: 1. Gene sequencing, which is expensive and expensive, and can only measure the glutamine content of pure protein, which is not suitable for routine testing of daily samples and enterprises. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6812
Inventor 陈淋敏吴海英蔡艳玲仲伟迎杨再龙丁能水吴有林
Owner FUJIAN AONONG BIOLOGICAL TECH GRP CO LTD
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