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A group of molecular markers related to the fiber diameter of long-haired rabbit and its application

A technology of molecular markers and long-haired rabbits, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, biochemical equipment and methods, etc., can solve the problems that there is no FZD3 gene alone or in combination with long-haired rabbit breeding, and achieve improvement Fluff fiber diameter-related traits, effect of improving rough hair fiber diameter-related traits

Active Publication Date: 2022-06-17
SHANDONG AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The structural and functional variations of the above genes are expected to be developed as molecular markers for rabbit hair fiber breeding. There is no report on the application of KRT26 gene and FZD3 gene alone or in combination in long-haired rabbit breeding.

Method used

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  • A group of molecular markers related to the fiber diameter of long-haired rabbit and its application
  • A group of molecular markers related to the fiber diameter of long-haired rabbit and its application
  • A group of molecular markers related to the fiber diameter of long-haired rabbit and its application

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Experimental program
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Embodiment

[0031] 1. Sample collection: The present invention uses long-haired rabbits jointly cultivated by Shandong Mengyin Yida Rabbit Co., Ltd. and Shandong Agricultural University as the test material, and the two batches of samples are 1009 in total. Raised under the same conditions, on the 283rd day after birth (the fourth shearing), the hair growth period was 73 days. Use scissors to close the skin and cut off the hair sample at the center of the body. Cut out a piece of ear tissue the size of a soybean grain, put the cut ear tissue into a 1.5mL centrifuge tube containing 75% alcohol, and take it back to the laboratory. , -20 ℃ for the extraction of genomic DNA. The reagents used in the experiments were purchased from TaKaRa and other companies.

[0032] 2. Experimental method: Genomic DNA was extracted from ear tissue samples by high-salt method, and the concentration and quality of DNA were detected by spectrophotometer. The extracted DNA was stored at -20°C.

[0033] 3. Poo...

experiment example

[0086] Experimental example, correlation analysis between hair fiber diameter traits of long-haired rabbits and FZD3 gene and KRT26 gene

[0087] The GLM program of SAS9.2 was used to analyze the variance, and the association analysis of each genotype of the polymorphic locus and the hair-yielding traits was carried out. The BLUP model is:

[0088] Y=Xb+Za+e

[0089] Y: phenotype value of hairy traits; X: matrix of number of individuals related to fixed effect; b: fixed effect (SNP locus, sex effect); Z: matrix of number of individuals with additive genetic effect of individual; a: additive of individual Genetic effects; e: random error.

[0090] According to GBT13835 "Rabbit Hair Fiber Test Method", the trait of hair fiber diameter was determined, and the allele and genotype frequencies were calculated according to the FZD3 gene SNPs and KRT26 gene SNPs, respectively, and the Hardy-Weinberg equilibrium test was performed on them to obtain the genotypes respectively. CC, CT...

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Abstract

The invention relates to the technical field of rabbit molecular marker breeding, in particular to a group of molecular markers related to the fiber diameter of long-haired rabbits and their applications. The molecular markers of the present invention include at least one of the mutants of the FZD3 gene and the mutant of the KRT26 gene One, the FZD3 gene is as shown in SEQ ID NO: 1, the KRT26 gene is as shown in SEQ ID NO: 2, the mutant of the FZD3 gene is formed by the base T mutation at the 41019916th place of the FZD3 gene to C Formed, the mutant of the KRT26 gene is formed by mutating the base A at the 41840359th place of the KRT26 gene to G; this group of molecular markers has a very significant genetic effect on the fiber diameter of the long-haired rabbit, respectively controlling the overall diameter of the rabbit hair fiber 1.47% and 2.40% of the genetic variation, the long-haired rabbit hair fiber diameter can be improved by selecting its favorable genotype.

Description

technical field [0001] The invention relates to the technical field of rabbit molecular marker breeding, in particular to a group of molecular markers related to the diameter of long-haired rabbit hair fibers and applications thereof. Background technique [0002] Rabbit hair fiber is an excellent textile raw material. Rabbit hair products are light in texture, smooth and soft to the touch, good in color, fleece effect and good in warmth retention, which are similar to wool fibers in many aspects. Rabbit hair fiber is rich in resources, the price is cheaper than wool fiber, and rabbit wool is more resistant to acid and alkali, and has advantages over cashmere in cleaning and dyeing. , With the improvement of wool spinning technology and the development of new products, especially the successful development of rabbit cashmere fabrics, the demand for rabbit cashmere in domestic and foreign markets has increased rapidly, and rabbit cashmere has become the second largest "gold f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/124C12Q2600/156C12Q2531/113C12Q2533/101C12Q2565/627
Inventor 樊新忠张露宋靓乔西波王云凤杨爱国杨远峰王召路
Owner SHANDONG AGRICULTURAL UNIVERSITY
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