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Group of molecular markers related to hair fiber diameter of long-hair rabbit and application of molecular markers

A molecular marker, long-haired rabbit technology, applied in recombinant DNA technology, microbial determination/inspection, biochemical equipment and methods, etc., can solve the problem of long-haired rabbit breeding without FZD3 gene alone or in combination

Active Publication Date: 2021-04-09
SHANDONG AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The structural and functional variations of the above genes are expected to be developed as molecular markers for rabbit hair fiber breeding. There is no report on the application of KRT26 gene and FZD3 gene alone or in combination in long-haired rabbit breeding.

Method used

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  • Group of molecular markers related to hair fiber diameter of long-hair rabbit and application of molecular markers
  • Group of molecular markers related to hair fiber diameter of long-hair rabbit and application of molecular markers
  • Group of molecular markers related to hair fiber diameter of long-hair rabbit and application of molecular markers

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Experimental program
Comparison scheme
Effect test

Embodiment

[0031] 1. Sample collection: In the present invention, long-haired rabbits jointly bred by Shandong Mengyin Yida Rabbit Industry Co., Ltd. and Shandong Agricultural University are used as test materials, and two batches of samples total 1009 rabbits. Raised under the same conditions, on the 283rd day after birth (the fourth shearing), the hair raising period was 73 days, and the indicators such as hair production, fiber diameter, and coarse hair rate were measured for each individual. Use scissors close to the skin to cut off the hair sample at the center of the side of the body, use surgical clipping to take a piece of ear tissue the size of a soybean grain, put the cut ear tissue into a 1.5mL centrifuge tube filled with 75% alcohol, and bring it back to the laboratory , stored at -20°C for extraction of genomic DNA. The reagents used in the experiment were purchased from companies such as TaKaRa.

[0032] 2. Experimental method: Genomic DNA was extracted from the collected ...

experiment example

[0086] Experimental example, association analysis of long-haired rabbit hair fiber diameter traits with FZD3 gene and KRT26 gene

[0087] The GLM program of SAS9.2 was used for analysis of variance, and the association analysis between the genotypes of the polymorphic loci and the hair-producing traits was carried out. The BLUP model is:

[0088] Y=Xb+Za+e

[0089] Y: phenotype value of hair-producing traits; X: individual number matrix related to fixed effects; b: fixed effects (SNP sites, sex effects); Z: individual number matrix of individual additive genetic effects; a: individual additive Genetic effect; e: random error.

[0090] According to GBT13835 "Rabbit Hair Fiber Test Method", the trait of hair fiber diameter is measured, and the allele and genotype frequencies are calculated according to the FZD3 gene SNPs and KRT26 gene SNPs respectively, and the Hardy-Weinberg balance test is carried out to obtain the genotype respectively CC, CT and TT; genotypes AA, AG and ...

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Abstract

The invention relates to the technical field of rabbit molecular marker breeding, in particular to a group of molecular markers related to the hair fiber diameter of a long-hair rabbit and application of the molecular markers. The molecular markers comprise at least one of a mutant of an FZD3 gene and a mutant of a KRT26 gene; the FZD3 gene is as shown in SEQ ID NO:1; the KRT26 gene is as shown in SEQ ID NO:2; the mutant of the FZD3 gene is formed by mutating a basic group T at the 41019916th position of the FZD3 gene into C; and the mutant of the KRT26 gene is formed by mutating a basic group A at the 41840359th position of the KRT26 gene into G. The group of molecular markers have an extremely remarkable genetic effect on the hair fiber diameter of the long-hair rabbit, 1.47% and 2.40% of the total genetic variation of the hair fiber diameter of the rabbit are controlled respectively, and the hair fiber diameter of the long-hair rabbit can be improved by selecting favorable genotypes.

Description

technical field [0001] The invention relates to the technical field of rabbit molecular marker breeding, in particular to a group of molecular markers related to long-haired rabbit hair fiber diameter and application thereof. Background technique [0002] Rabbit hair fiber is an excellent textile raw material. Rabbit hair products are light in texture, smooth and soft to the touch, good in color, good in fleece effect and good in warmth retention. It is similar to wool fiber in many aspects. Rabbit hair fiber is rich in resources, and the price is cheaper than wool fiber, and rabbit hair is more resistant to acid and alkali, and has advantages over cashmere in cleaning and dyeing. , with the improvement of wool spinning technology and the development of new products, especially the successful development of rabbit velvet fabrics, the demand for rabbit velvet in domestic and foreign markets has increased rapidly, and rabbit velvet has become the second largest "golden fiber" ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/124C12Q2600/156C12Q2531/113C12Q2533/101C12Q2565/627
Inventor 樊新忠张露宋靓乔西波王云凤杨爱国杨远峰王召路
Owner SHANDONG AGRICULTURAL UNIVERSITY
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