Molecular marker related to wool yield of Angora rabbits and application thereof

A molecular marker, long-haired rabbit technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., which can solve the problems of reduced wool spinning value, low selection accuracy, and reduced textile performance.

Active Publication Date: 2021-03-23
SHANDONG AGRICULTURAL UNIVERSITY +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Conventional breeding methods are affected by the scale of performance measurement, the standardization of performance measurement, genetic evaluation methods, etc., and the selection accuracy is relatively low
Moreover, when choosing to increase the wool production, it is difficult to take into account the relevant selection responses to the quality traits of rabbit hair, which often leads to the increase of wool production, the increase of rabbit hair fiber diameter, the decline of textile performance, and the adverse consequences of wool spinning value reduction.

Method used

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  • Molecular marker related to wool yield of Angora rabbits and application thereof
  • Molecular marker related to wool yield of Angora rabbits and application thereof
  • Molecular marker related to wool yield of Angora rabbits and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1, long-haired rabbit KRT26 gene mutant detection

[0030] 1. Experimental material: The present invention uses long-haired rabbits jointly cultivated by Shandong Mengyin Yida Rabbit Industry Co., Ltd. and Shandong Agricultural University as the experimental material, and the samples are 757 in total. On the 283rd day after birth (the fourth shearing), the wool was raised under uniform conditions for 73 days, and the individual hair production and fiber quality indicators were measured. Use scissors close to the skin to cut off the hair sample at the center of the body, use surgical scissors to cut a piece of ear tissue the size of a soybean grain, put the cut ear tissue into a 1.5mL centrifuge tube filled with 75% alcohol, and bring it back to the test Room, stored at -20°C, for extraction of genomic DNA. The reagents used in the experiment were purchased from companies such as TaKaRa.

[0031] 2. Experimental method: Genomic DNA was extracted from the col...

Embodiment 2

[0041] Embodiment 2, SNP typing

[0042] SNP typing was performed by flight mass spectrometry, which was completed by Beijing Compson Biotechnology Co., Ltd. The specific steps are as follows:

[0043] (1) Primer design: According to the SNP site information, use the MassARRAY company design software AssayDesignSuitev2.0 to design PCR reactions and single-base extension primers, and check the primer specificity online through UCSC.

[0044] (2) Genomic DNA quality inspection: Detect the DNA concentration, purity and degradation degree by agarose gel electrophoresis, and the test result interpretation standard: In the electrophoresis detection gel, the DNA band is single, clear, free of impurities, without dispersion and tailing .

[0045] (3) Electrophoresis conditions:

[0046] ①0.8% agarose gel, 170V, 25min

[0047] ② Loading volume: 500ng sample + 3μl Loading Buffer

[0048] ③Marker: Trans2000 Plus 3μl

[0049] (4) PCR reaction:

[0050] ①The PCR reaction system is sh...

experiment example

[0076] Experimental example, association analysis of hair yield traits and KRT26 gene in long-haired rabbits

[0077] The GLM program of SAS9.2 was used for analysis of variance, and the correlation analysis was carried out between the genotypes of polymorphic sites and the traits of hair yield in long-haired rabbits. The BLUP model is:

[0078] Y=Xb+Za+e

[0079] Y: phenotype value of hair-producing traits; X: individual number matrix related to fixed effects; b: fixed effects (SNP sites, sex effects); Z: individual number matrix of individual additive genetic effects; a: individual additive Genetic effect; e: random error; allele and genotype frequencies were calculated according to the SNPs of the KRT26 gene, and the genotypes AA, AG, and GG were obtained respectively.

[0080] Table 8 Results of association analysis between KRT26 gene and wool yield traits of long-haired rabbits

[0081]

[0082] From the data in Table 8, it can be shown that the 41844263 position of...

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Abstract

The invention relates to the technical field of rabbit molecular marker breeding, and particularly relates to a molecular marker related to the wool yield of Angora rabbits and breeding application thereof, the molecular marker comprises a mutant of a KRT26 gene, the KRT26 gene is shown as SEQ ID NO: 1, and the mutant of the KRT26 gene is formed by basic group G->A mutation at the 41844263rd siteof the KRT26 gene; according to the invention, correlation analysis and population verification are carried out on the wool yield traits of the Angora rabbits and the KRT26 gene, it is found that thewool yield of Angora rabbit individuals with allelic genes G is higher, with the additive gene effect being 15.59 g, and the basepair substitution can control the total genetic variation of the wool yield by 1.51%. In the breeding work, the wool yield traits of the Angora rabbits can be improved by increasing the gene frequency of the allele G in the Angora rabbit population, and the molecular marker can be used as an auxiliary selection marker of the wool yield traits of the Angora rabbits.

Description

technical field [0001] The invention relates to the technical field of rabbit molecular marker breeding, in particular to a molecular marker related to wool production of long-haired rabbits and its breeding application. Background technique [0002] Rabbit hair has the characteristics of slender and soft fibers, fluffy and light texture, and good warmth retention. It is a high-grade textile material with excellent texture and occupies an important position in the textile industry. With the improvement of wool spinning technology and the development of new products, the demand for rabbit fur / velvet in domestic and foreign markets is increasing rapidly. my country is the country with the largest breeding volume of long-haired rabbits in the world, but the wool yield of a single long-haired rabbit is low, so it is of great significance to carry out breeding of long-haired rabbits with high wool yield. [0003] The conventional breeding method to improve the wool yield of long...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124C12Q1/686C12Q1/6876
Inventor 樊新忠宋靓乔西波杨爱国杨远峰张露王召路
Owner SHANDONG AGRICULTURAL UNIVERSITY
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