Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Blood exosome marker for judging buffalo birth time and application of blood exosome marker

A marker, extra-blood technology, used in biology

Active Publication Date: 2021-04-09
GUANGXI ZHUANG AUTONOMOUS REGION BUFFALO INST
View PDF17 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The study of the formation process of the calf's autogenous immune system is still in its infancy, and there are still many urgent questions to be solved

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Blood exosome marker for judging buffalo birth time and application of blood exosome marker
  • Blood exosome marker for judging buffalo birth time and application of blood exosome marker
  • Blood exosome marker for judging buffalo birth time and application of blood exosome marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Reagents and instruments:

[0023] EDTA anticoagulant blood collection tubes were purchased from BD Company of the United States; L-100XP cryogenic ultracentrifuge was purchased from Beckman Company of the United States; Total Exosome Isolation (00520032) was purchased from Invitrogen Company; transmission electron microscope (H7650) was purchased from Hitachi Company of Japan; The potentiometer (Nano ZS90) was purchased from Malvern Company, UK; PBS buffer was purchased from Biological Industries, Israel; Calnexin antibody (AC018-1), TSG101 antibody (AF8259), CD9 antibody (AF1192) and CD81 antibody (AF2428) were purchased from Shanghai Biyuntian Biotechnology Co., Ltd.

[0024] 2. Time-sharing sampling:

[0025] Select 9 birthed Mora buffalo calves, and sample them every 2 hours within 24 hours of birth, and sample once a day on the 2nd day of birth until the 14th day after birth; the collection process is as follows:

[0026] Use EDTA anticoagulant blood collecti...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention relates to the technical field of biology, in particular to a blood exosome marker for judging buffalo birth time and application of the blood exosome marker. According to the application, specific research is carried out on the newborn calves, a whole blood sample is mainly adopted to carry out long-term research on the establishment process of a buffalo immune system by researching exosomes in the sample, and along with the establishment process of the buffalo immune system, the protein contents and types of the exosomes of the newborn calves are greatly different, the birth time of the calves can be divided into within 2 hours of birth, 3 hours of birth and more than 12 days of birth according to the change condition of exosomes, the birth time of the calves can be effectively judged by detecting the yin and yang of Calnexin, TSG101, CD9 and CD81 proteins, and a good indication effect is achieved for later immune research, management, feeding and the like.

Description

【Technical field】 [0001] The invention relates to the field of biotechnology, in particular to blood exosome markers for judging the birth time of buffaloes and applications thereof. 【Background technique】 [0002] Exosomes, also known as multivesicular bodies (MVB), are a type of tiny vesicles secreted by cells with a diameter of 30 to 150 nm and a single-layer membrane structure. In biological plasma, urine, saliva, ascites, amniotic fluid and other body fluids. In recent years, with the deepening of research, the understanding of the structural size and shape of exosomes has gradually become clear, but the molecular mechanisms involved in the physiological processes and the functional research of exosome contents are still in their infancy. The species involved in the study is also still limited. [0003] At present, exosomes contain various active substances such as protein, DNA, mRNA and some non-coding RNA, and the structural size, shape and density of exosomes are m...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/53
CPCG01N33/6854G01N33/53G01N2333/70596G01N2333/4727
Inventor 庞春英梁贤威梁莎莎文崇利陈明棠韦科龙陆呈委潘玉红
Owner GUANGXI ZHUANG AUTONOMOUS REGION BUFFALO INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com